Versatile Patterns in the Actin Cortex of Motile Cells: Self-Organized Pulses Can Coexist with Macropinocytic Ring-Shaped Waves

Yochelis, Arik; Flemming, Sven; Beta, Carsten

doi:10.1103/physrevlett.129.088101

Cited by 8 publications

(8 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An explicit inhibitor can be added to the ADS model as shown in Figure 2A and the resulting molecular mechanism is then described by a three-variable activator-depleted substrateinhibitor (ADSI) model. This circuit consists of the activator (RT)-depleted substrate (RD) and the activator (RT)-inhibitor (F) submodules linked by a common activator, a layout often inferred in systems exhibiting complex oscillatory dynamics [30][31][32] . However, in the framework of the ADSI model 3,4 , depletion of inactive RhoA does not play a key role in the generation of waves, which are instead produced by the action of the inhibitor, F (Figure 2A).…”

Section: Testing Theoretical Models Of Rhoa Wave Generationmentioning

confidence: 99%

Testing models of cell cortex wave generation by Rho GTPases

Chomchai,

Leda,

Golding

et al. 2024

Preprint

View full text Add to dashboard Cite

The Rho GTPases pattern the cell cortex in a variety of fundamental cell-morphogenetic processes including division, wound repair, and locomotion. It has recently become apparent that this patterning arises from the ability of the Rho GTPases to self-organize into static and migrating spots, contractile pulses, and propagating waves in cells from yeasts to mammals1. These self-organizing Rho GTPase patterns have been explained by a variety of theoretical models which require multiple interacting positive and negative feedback loops. However, it is often difficult, if not impossible, to discriminate between different models simply because the available experimental data do not simultaneously capture the dynamics of multiple molecular concentrations and biomechanical variables at fine spatial and temporal resolution. Specifically, most studies typically provide either the total Rho GTPase signal or the Rho GTPase activity as reported by various sensors, but not both. Therefore, it remains largely unknown how membrane accumulation of Rho GTPases (i.e., Rho membrane enrichment) is related to Rho activity. Here we dissect the dynamics of RhoA by simultaneously imaging both total RhoA and active RhoA in the regime of acute cortical excitability2, characterized by pronounced waves of Rho activity and F-actin polymerization3-5. We find that within nascent waves, accumulation of active RhoA precedes that of total RhoA, and we exploit this finding to distinguish between two popular theoretical models previously used to explain propagating cortical Rho waves.

show abstract

Section: Testing Theoretical Models Of Rhoa Wave Generationmentioning

confidence: 99%

Testing models of cell cortex wave generation by Rho GTPases

Chomchai,

Leda,

Golding

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…a co-moving coordinate frame transformation), where

is a propagation speed. Examples in the context of actin waves include Yochelis et al, 2020 ; Champneys et al, 2021 ; Yochelis et al, 2022 .…”

Section: Mathematical Modeling Approachesmentioning

confidence: 99%

From actin waves to mechanism and back: How theory aids biological understanding

Beta

Edelstein‐Keshet

Gov

et al. 2023

eLife

Self Cite

View full text Add to dashboard Cite

Actin dynamics in cell motility, division, and phagocytosis is regulated by complex factors with multiple feedback loops, often leading to emergent dynamic patterns in the form of propagating waves of actin polymerization activity that are poorly understood. Many in the actin wave community have attempted to discern the underlying mechanisms using experiments and/or mathematical models and theory. Here, we survey methods and hypotheses for actin waves based on signaling networks, mechano-chemical effects, and transport characteristics, with examples drawn from Dictyostelium discoideum, human neutrophils, Caenorhabditis elegans, and Xenopus laevis oocytes. While experimentalists focus on the details of molecular components, theorists pose a central question of universality: Are there generic, model-independent, underlying principles, or just boundless cell-specific details? We argue that mathematical methods are equally important for understanding the emergence, evolution, and persistence of actin waves and conclude with a few challenges for future studies.

show abstract

“…The importance of conservation laws for pattern formation has been widely discussed: Matthews & Cox [11] and Winterbottom et al [12] analyse small-scale stationary and oscillatory instabilities in the presence of a conservation law, which are relevant for pattern formation in a wide spectrum of systems, e.g. in magnetoconvection [13], in crystallization of passive and active colloids [14,15] and in the dynamics of the actin cortex of motile cells [16,17]. It has been recently shown that the standard CH model (introduced to describe phase separation in a binary mixture [18,19]) also corresponds to an amplitude equation valid close to a large-scale stationary instability in systems with a single conservation law [20].…”

Section: Introductionmentioning

confidence: 99%

Non-reciprocity induces resonances in a two-field Cahn–Hilliard model

Frohoff-Hülsmann

Thiele

Pismen

2023

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

We consider a non-reciprocally coupled two-field Cahn–Hilliard system that has been shown to allow for oscillatory behaviour and suppression of coarsening. After introducing the model, we first review the linear stability of steady uniform states and show that all instability thresholds are identical to the ones for a corresponding two-species reaction–diffusion system. Next, we consider a specific interaction of linear modes—a ‘Hopf–Turing’ resonance—and derive the corresponding amplitude equations using a weakly nonlinear approach. We discuss the weakly nonlinear results and finally compare them with fully nonlinear simulations for a specific conserved amended FitzHugh–Nagumo system. We conclude with a discussion of the limitations of the employed weakly nonlinear approach. This article is part of the theme issue ‘New trends in pattern formation and nonlinear dynamics of extended systems’.

show abstract

Versatile Patterns in the Actin Cortex of Motile Cells: Self-Organized Pulses Can Coexist with Macropinocytic Ring-Shaped Waves

Cited by 8 publications

References 70 publications

Testing models of cell cortex wave generation by Rho GTPases

Testing models of cell cortex wave generation by Rho GTPases

From actin waves to mechanism and back: How theory aids biological understanding

Non-reciprocity induces resonances in a two-field Cahn–Hilliard model

Contact Info

Product

Resources

About