Versatile roles of plant NADPH oxidases and emerging concepts

Kaur, Gurpreet; Sharma, Ashutosh; Guruprasad, Kunchur; Pati, Pratap Kumar

doi:10.1016/j.biotechadv.2014.02.002

Cited by 119 publications

(107 citation statements)

References 98 publications

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“…Rboh is involved in many plant processes including cell growth (Foreman et al 2003), plant development, stomatal closure (Shi et al 2012), pollen tube growth (Kaya et al 2014), symbiotic interactions (Marino et al 2012;Kaur et al 2014), abiotic stress, and pathogen response (Wojtaszek 1997;Torres et al 2002;Daudi et al 2012;Siddique et al 2014). However, the number of Rboh isozymes which are differentially expressed suggests a certain grade of specialization for each one.…”

Section: Plasma Membranementioning

confidence: 99%

“…Besides the mechanism of the local production of O 2 •À by Rboh, it has been proposed that after some stimuli (i.e., pathogens) and the generation of a local burst of ROS mediated by Rboh in an specific cells, there is a cascade of cell-to-cell communication events that carries a systemic signal over long distances throughout different tissues of the plants (see chapter "ROS as Key Players of Abiotic Stress Responses in Plants" of this book by Suzuki for deeper discussion) which opens a new perspective of the Rboh functions (Marino et al 2012;Kaur et al 2014). …”

Section: Plasma Membranementioning

confidence: 99%

See 1 more Smart Citation

Production Sites of Reactive Oxygen Species (ROS) in Organelles from Plant Cells

Corpas

Gupta

Palma

2015

Reactive Oxygen Species and Oxidative Damage in Plants Under Stress

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Section: Plasma Membranementioning

confidence: 99%

Section: Plasma Membranementioning

confidence: 99%

Production Sites of Reactive Oxygen Species (ROS) in Organelles from Plant Cells

Corpas

Gupta

Palma

2015

Reactive Oxygen Species and Oxidative Damage in Plants Under Stress

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“…Reports on their role in the defense response and vital morphogenetic processes such as cell expansion, pollen tube growth, seed germination and after-ripening, root hair elongation, Ca 2+ -dependent stomatal closure have been documented (Kaur et al 2014). The first plant NADPH oxidase was identified in Oryza sativa, called as OsRbohA (Groom et al 1996).…”

Section: Introductionmentioning

confidence: 99%

“…The domain architecture comprises an extended N-terminal region containing two Ca 2+ -binding EF-hand motifs, six a-helical transmembrane domains (TMD-I to TMD-VI) connected by five loops (loops A-E) and a C-terminal FNR (ferredoxin-NADP + reductase) domain containing FAD (flavin adenine dinucleotide) and NADPH-binding moieties (Kaur et al 2014). Experimental evidence suggest that the N-terminal region plays a key role in the regulation of Rbohs via Ca 2+ , Rac GTPase, protein kinases (CDPK; calcium-dependent protein kinase, CcaMK; Ca 2+ /CaM-dependent protein kinase, MAPK; mitogen activated protein kinase, OST1; open stomata 1, receptor-like cytoplasmic kinase BIK1, histidine kinase), GIRAFFE heme oxygenase, extracellular ATP, phospholipase Dα1, and phosphatidic acid (Kaur et al 2014; Baxter et al 2014). The N-terminal region of RbohB from O. sativa (OsRbohB 138–313 ) comprises a homodimer, where each monomer contains two EF-hands and two EF-hand-like motifs with Ca 2+ -binding sites (Oda et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Structural complexity and functional diversity of plant NADPH oxidases

et al. 2017

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Plant NADPH oxidases also known as respiratory burst oxidase homologs (Rbohs) are a family of membrane-bound enzymes that play diverse roles in the defense response and morphogenetic processes via regulated generation of reactive oxygen species. Rbohs are associated with a variety of functions, although the reason for this is not clear. To evaluate using bioinformatics, the possible mechanisms for the observed functional diversity within the plant kingdom, 127 Rboh protein sequences representing 26 plant species were analyzed. Multiple clusters were identified with gene duplications that were both dicot as well as monocot-specific. The N-terminal sequences were observed to be highly variable. The conserved cysteine (equivalent of Cys890) in C-terminal of AtRbohD suggested that the redox-based modification like S-nitrosylation may regulate the activity of other Rbohs. Three-dimensional models corresponding to the N-terminal domain for Rbohs from Arabidopsis thaliana and Oryza sativa were constructed and molecular dynamics studies were carried out to study the role of Ca in the folding of Rboh proteins. Certain mutations indicated possibly affect the structure and function of the plant NADPH oxidases, thereby providing the rationale for further experimental validation.

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“…RBOHs are calcium-dependent NADPH oxidases that generates superoxide ions and implicated in versatile roles in plants [41]. Previously, dual role of the AtRbohD in response to Alternaria has been reported in Arabidopsis , where it triggers cell death in infected cells as well as it simultaneously suppresses the cell-death in neighboring healthy cells [42].…”

Section: Discussionmentioning

confidence: 99%

Changes in the leaf proteome profile of Withania somnifera (L.) Dunal in response to Alternaria alternata infection

Singh

Joshi

et al. 2017

PLoS ONE

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Withania somnifera is a high value medicinal plant which is used against large number of ailments. The medicinal properties of the plant attributes to a wide array of important secondary metabolites. The plant is predominantly infected with leaf spot pathogen Alternaria alternata, which leads to substantial biodeterioration of pharmaceutically important metabolites. To develop an effective strategy to combat this disease, proteomics based approach could be useful. Hence, in the present study, three different protein extraction methods tris-buffer based, phenol based and trichloroacetic acid-acetone (TCA-acetone) based method were comparatively evaluated for two-dimensional electrophoresis (2-DE) analysis of W. somnifera. TCA-acetone method was found to be most effective and was further used to identify differentially expressed proteins in response to fungal infection. Thirty-eight differentially expressed proteins were identified by matrix assisted laser desorption/ionization time of flight-mass spectrometry (MALDI TOF/TOF MS/MS). The known proteins were categorized into eight different groups based on their function and maximum proteins belonged to energy and metabolism, cell structure, stress and defense and RNA/DNA categories. Differential expression of some key proteins were also crosschecked at transcriptomic level by using qRT-PCR and were found to be consistent with the 2-DE data. These outcomes enable us to evaluate modifications that take place at the proteomic level during a compatible host pathogen interaction. The comparative proteome analysis conducted in this paper revealed the involvement of many key proteins in the process of pathogenesis and further investigation of these identified proteins could assist in the discovery of new strategies for the development of pathogen resistance in the plant.

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Versatile roles of plant NADPH oxidases and emerging concepts

Cited by 119 publications

References 98 publications

Production Sites of Reactive Oxygen Species (ROS) in Organelles from Plant Cells

Production Sites of Reactive Oxygen Species (ROS) in Organelles from Plant Cells

Structural complexity and functional diversity of plant NADPH oxidases

Changes in the leaf proteome profile of Withania somnifera (L.) Dunal in response to Alternaria alternata infection

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