Verschiebungen in den IgG-Subklassen G1 und G2 bei malignen Erkrankungen

Schauenstein, E.; Reiter, Monika; Leitsberger, A.; Gombotz, Hans; List, W. F.

doi:10.1007/bf00367201

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…We have previously reported that human IgG contains definite and consistent numbers of free sulfhydryl (SH) groups and labile disulfide bonds (SS*) that are detectable by disulfide exchange reaction with 5,5'-dithio-2,2'-dinitrobenzoic acid (dithionitrobenzoate; DTNB) [1][2][3][4]. In vestigations with purified subclass proteins revealed IgGl to carry 1 SS*/mol, whereas 1 SH could be assigned to the IgG2 molecule [3].…”

Section: Introductionmentioning

confidence: 99%

“…This corre sponded to a decrease in the relative concentrations of IgGl from 60-70% down to 46 ±5% and to an increase of IgG2 from 20-30% to 40 ± 5%, as determined by quantita tive immunoprécipitation techniques [9]. No significant changes in the IgG subclass profile were noted in about 400 cases with benign diseases of the respective organ sys tems [3][4][5][6],…”

Section: Introductionmentioning

confidence: 99%

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A Decrease in Reactive Disulfide Bonds of Serum IgG Signals a Characteristic Change in the IgG Subclass Pattern of Rats Bearing Experimental Tumors

Weblacher¹,

Leitsberger²,

Tillian³

et al. 1993

Int Arch Allergy Immunol

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Previous studies have shown that human IgG1 contains a ‘reactive’ disulfide bridge (SS*), detectable by a 24-hour disulfide exchange reaction, and that the serum level of this IgG subclass is selectively diminished in patients with various malignant diseases. Here we present evidence that in rats IgG2b is the only subclass that carries one SS* per molecule. Furthermore, it is shown that rats inoculated with experimental tumor lines, i.e., the Yoshida hepatoma ascites tumor and the Walker 256 carcinosarcoma growing in ascites or as solid tumor, exhibit significantly decreased SS* per mole IgG which corresponds to a selective diminution of IgG2b. Although at later stages there is a quantitative correlation with the tumor burden, with the Walker tumor this effect becomes significant as early as 24 h after inoculation, i.e., well before exponential tumor growth and an absolute reduction of total IgG. Control animals injected intraperitoneally with either viable spleen cells or irradiated Walker 256 cells did not show comparable alterations in their IgG subclass profile. Thus, the selective defect of IgG2b requires the presence of viable and proliferating tumor cells. Possible mechanism(s) of tumor-associated shifts in IgG subclasses are discussed.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Decrease in Reactive Disulfide Bonds of Serum IgG Signals a Characteristic Change in the IgG Subclass Pattern of Rats Bearing Experimental Tumors

Weblacher¹,

Leitsberger²,

Tillian³

et al. 1993

Int Arch Allergy Immunol

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ΣS, a measure of reactive sulfur groups of immunoglobulin G, is a sensitive tumor marker discriminating different stages of breast cancer

Smola

Estelberger

Reiter

et al. 1991

Cancer

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ΣS is a measure of the disulfide bonds and free thiol groups of serum immunoglobulin (Ig) G, as determined by the reaction with dithionitrobenzoate. Significant decreases of 2S previously were detected in malignant compared with benign diseases of various organs. This study shows the application of ΣS for the diagnosis of breast cancer. The following results were obtained. First, 132 patients with benign breast diseases showed a ΣS of 1.48 ± 0.29 (standard deviation) per mole IgG; this was not different from 1.51 ± 0.36 found in 182 controls. In contrast, IgG from 198 patients with primary breast carcinoma of all four stages (tumor‐node‐metastasis system) gave a ΣS of 1.22 ± 0.29, a significant (P less than 0.0001) decrease of ΣS from benign to malignant breast disease. Second, ΣS values of single Stages I, II, III, and IV, were 1.27 (n = 59), 1.23 (n = 83), 1.19 (n = 35), and 1.10 (n = 21), respectively, each significantly different from ΣS in benign disease and showing a decreasing trend with increasing tumor progress. Differences were significant between Stages I and IV (P less than 0.025) and II and IV (P less than 0.05). Third, 63% of Stage I breast carcinoma patients had ΣS values below a critical threshold of 1.38. This serum positivity rose to 90% in Stage IV. These values exceeded those reported with other tumor markers. The overall power of ΣS to distinguish between benign and malignant breast disease had a specificity of 61% and a sensitivity of 78%. Early stages (I and II) of breast cancer could be distinguished from benign diseases with 64% specificity and 69% sensitivity. Advanced Stage IV could be discriminated from early Stages I and II with 55% specificity and 71% sensitivity. Thus, the analysis of ΣS may significantly contribute to the surveillance of patients with breast cancer.

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Microphotometric Determination of Protein Thiols and Disulphides in Tissue Samples from the Human Uterine Cervix and the Skin Reveal a “Field Effect” in the Surroundings of Benign and Malignant Tumours

Nöhammer

Bajardi²,

Benedetto

et al. 1988

Eicosanoids, Lipid Peroxidation and Cancer

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Verschiebungen in den IgG-Subklassen G1 und G2 bei malignen Erkrankungen

Cited by 3 publications

References 7 publications

A Decrease in Reactive Disulfide Bonds of Serum IgG Signals a Characteristic Change in the IgG Subclass Pattern of Rats Bearing Experimental Tumors

A Decrease in Reactive Disulfide Bonds of Serum IgG Signals a Characteristic Change in the IgG Subclass Pattern of Rats Bearing Experimental Tumors

ΣS, a measure of reactive sulfur groups of immunoglobulin G, is a sensitive tumor marker discriminating different stages of breast cancer

Microphotometric Determination of Protein Thiols and Disulphides in Tissue Samples from the Human Uterine Cervix and the Skin Reveal a “Field Effect” in the Surroundings of Benign and Malignant Tumours

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