Vertical distribution of bacterial community structure in the sediments of two eutrophic lakes revealed by denaturing gradient gel electrophoresis (DGGE) and multivariate analysis techniques

Abstract

Vertical distribution of bacterial community structure was investigated in the sediments of two eutrophic lakes of China, Lake Taihu and Lake Xuanwu. Profiles of bacterial communities were generated using a molecular fingerprinting technique, denaturing gradient gel electrophoresis (DGGE) followed by DNA sequence analysis, and the results were interpreted with multivariate statistical analysis. To assess changes in the genetic diversity of bacterial communities with changing depth, DGGE banding patterns were analysed by cluster analysis. Distinct clusters were recognized in different sampling stations of Lake Taihu. Canonical correspondence analysis (CCA) was carried out to infer the relationship between environmental variables and bacterial community structure. DGGE samples collected at the same sampling site clustered together in both lakes. Total phosphorus, organic matter and pH were considered to be the key factors driving the changes in bacterial community composition.

Seasonal and Spatial Changes of Planktonic Bacterial Communities Inhabiting the Natural Thermal Lake Hévíz, Hungary

“…This method provides the possibility to identify the community members by reamplifying and sequencing of the excised bands. Previously, DGGE technique was successfully applied to reveal the alterations of bacterial or total microbial communities in freshwater and seawater habitats infl uenced by different environmental factors [10,11,12,13,14]. According to the UPGMA dendrogram based on the DGGE patterns, signifi cant seasonal changes with less spatial differences were found among the total planktonic bacterial communities of Lake Hévíz (Fig.…”

Effects of Heavy Metal and Nutrients on Benthic Microbial Communities in Freshwater Sediment of Poyang Lake (China)

“…Notably, TP, Cu and Cd were important factors affecting bacterial community in surface sediment of Poyang Lake. TP played an important role in driving the changes in water column and sediment bacterial communities of freshwater Lake [4,15]. And it has been reported that phosphorus concentration is associated with the phosphate-dissolving and phosphate-decomposing bacteria in the sediments [19].…”

“…Huang et al [3] found that distribution of bacteria was affected by the combined effects of various dissolved inorganic matter in sediments of Pearl River, China. Total phosphorus and pH were characterized as significant environmental factors correlated with bacterial communities in the sediment of eutrophic lake [4]. Furthermore, it was reported that heavy metals have accumulative toxic effects on cells and they tend to be bound on sediments and removed from the water [5].…”

Sources of organic matter affect depth-related microbial community composition in sediments of Lake Erhai, Southwest China

“…4 B,C). pH may not only reflect changes in other environmental factors (KoskiVähälä et al, 2001), which can have both inhibitory and stimulative effects on bacterial communities (Zeng et al, 2009), but may also influence bacterial communities through direct biological mechanisms (Yannarell and Triplett, 2005).…”