Vertical flow immunoassay (VFA) biosensor for a rapid one-step immunoassay

Oh, Young Kyoung; Joung, Hyou-Arm; Kim, Sang-Hyo; Kim, Min-Gon

doi:10.1039/c2lc41016h

Cited by 96 publications

(75 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With a single test, they can measure only part of the range of relevant CRP concentrations 4,15–17 . For this reason, CRP diagnostics are sometimes called high-senstitivity CRP (or hsCRP) tests, and typically measure in the 1–10µg/ml range.…”

mentioning

confidence: 99%

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics

et al. 2017

View full text Add to dashboard Cite

The quantification of analyte concentrations using lateral flow assays is a low-cost and user-friendly alternative to traditional lab-based assays. However, sandwich-type immunoassays are often limited by the high-dose hook effect, which causes falsely low results when analytes are present in very high concentrations. In this paper, we present a reaction kinetics-based technique that solves this problem, significantly increasing the dynamic range of these devices. With the use of a traditional sandwich lateral flow immunoassay, a portable imaging device, and a mobile interface, we demonstrate the technique by quantifying C-reactive protein concentrations in human serum over a large portion of the physiological range. The technique could be applied to any hook effect-limited sandwich lateral flow assay and has a high level of accuracy even in the hook effect range.

show abstract

mentioning

confidence: 99%

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics

et al. 2017

View full text Add to dashboard Cite

show abstract

“…This is the application of a biosensor point-of-care-testing system with a detection limit comparable to that of ELISA. Oh et al [22] has recently developed a one-step biosensor for hsCRP detection using a vertical flow immunoassay. It is composed of a sample pad, flow through films (FTH), conjugate pad and nitrocellulose membranes (onto which anti-hsCRP and secondary antibodies are immobilized below the holes) which are stacked upon one another.…”

Section: Resultsmentioning

confidence: 99%

“…Cowles �� al [9] , 20 0 RNA aptamer based technology Uses Carbon nanotube's interdigitated electrodes of capacitors; highly selecti�e Qureshi �� al [20] , 202 Biosensor using FET In�ol�es SBP linked in protein A; point of care testing system; on-site analysis Kim �� al [2] , 20 2 Vertical flow Immunoassay One-step assay; time taken 2 min; most rapid; employs gold nanoparticles Oh �� al [22] , 20 Electrochemical impedance spectroscopy Most ad�anced technique; uses gold and diamond spray in fabrication; highly sensiti�e; reusable without sensiti�ity being lost; good detection limit Bryan �� al [2] , 20 …”

Section: Resultsmentioning

confidence: 99%

Prospects and advancements in C-reactive protein detection

Chandra

Suman

Airon

et al. 2014

WJM

View full text Add to dashboard Cite

C-reactive protein (CRP) is one o� the earliest proteins that appear in the blood circulation in most systemic in�lammatory conditions and this is the reason �or its signi�icance, even a�ter identi�ication o� many organ speci�ic in�lammatory markers which appear relatively late during the course o� disease. Earlier methods o� CRP detection were based on the classical methods o� antigen-antibody interaction through precipitation and agglutination reactions. Later on, CRP based enzymatic assays came into the picture which were �urther modi�ied by integration o� an antigen-antibody detection system with sur�ace plasma spectroscopy. Then came the time �or the development o� electrochemical biosensors where nanomaterials were used to make a highly sensitive and portable detection system based on silicon nanowire, metal-oxide-semiconductor �ield-e��ect transistor/bipolar junction transistor, ZnS nanoparticle, aptamer, �ield emission transmitter, vertical �low immunoassay etc . This editorial attempts to summarize developments in the �ield o� CRP detection, with a special emphasis on biosensor technology. This would help in translating the latest development in CRP detection in the clinical diagnosis o� in�lammatory conditions at an early onset o� the diseases. Key words: C-reactive protein; In�lammation; Diagnostic methods; Antibody; Biosensors Core tip: Over time, C-reactive protein (CRP) has emerged as a versatile marker �or the detection o� systemic in�lammatory conditions, providing preliminary in�orma-tion to clinicians �or continuing with a more speci�ic diagnostic methodology. Advancements in electroanalytical chemistry and knowledge o� nanomaterials have helped modern age researchers to miniaturize detection systems with an enhanced level o� speci�icity and sensitivity o� CRP detection. Further research should be directed in this area to devise a better diagnostic plat�orm that can detect the change in CRP level at a very early stage o� the onset o� in�lammatory conditions. Chandra P, Suman P, Airon H, Mukherjee M, Kumar P. Prospects and advancements in C-reactive protein detection. World

show abstract

“…[39,40] The use of this class of NPs in disease diagnostics runs the gamut from assays that rely on the high molar extinction coefficient of AuNPs as colorimetric labels to those which take advantage of the plasmonically amplified electric fields used by SERS for the ultralow quantification of biomarkers. [31,32, [69][70][71][72] As an example, we highlight a relatively new technology, the vertical flow assay (VFA), [73,74] which is proving to be a simple, rapid, and field deployable method to detect disease markers. In short, VFA strongly resembles a chemical analysis technology known as colorimetric-solid phase extraction (C-SPE).…”

Section: Plasmonics-based Detectionmentioning

confidence: 99%

Advantages and limitations of nanoparticle labeling for early diagnosis of infection

Laurentius

Owens

Park

et al. 2016

Expert Review of Molecular Diagnostics

View full text Add to dashboard Cite

This review examines the advantages and limitations of in vitro health care diagnostic tests that utilize nanoparticles (e.g. noble metal, quantum dot, and magnetic). It includes a brief overview of their unique properties, syntheses, and applicable readout strategies. This is followed by a brief synopsis of the obstacles faced when attempting to translate nanoparticle-based diagnostics from the R&D laboratory to the clinic and other arenas (i.e. the difficulties common to in vitro diagnostics), and then by a much more in-depth examination of the need to control and characterize a range of nanoparticle properties (e.g. size, shape, surface composition, and stability) when making this transition. Expert commentary: The review wraps up with a short commentary and perspective for the next five years, focusing on possible guidelines for nanoparticle characterization.

show abstract

Vertical flow immunoassay (VFA) biosensor for a rapid one-step immunoassay

Cited by 96 publications

References 26 publications

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics

Prospects and advancements in C-reactive protein detection

Advantages and limitations of nanoparticle labeling for early diagnosis of infection

Contact Info

Product

Resources

About