Vertical Immobilization of Viable Bacilliform Bacteria into Polypyrrole Films

Tokonami, Shinji; Saimatsu, Kenta; Nakadoi, Yuh; Furuta, Mamoru; Shiigi, Hiroshi; Nagaoka, Tsutomu

doi:10.2116/analsci.28.319

Cited by 29 publications

(12 citation statements)

References 18 publications

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“…[1][2][3][4][5] In this connection, we have developed a quartz crystal-microbalance (QCM) based real-time bacterial sensor using a polymer film imprinted with the targeted bacteria. 6,7 However, as far as field applications are concerned, QCM detectors have some issues to be resolved regarding externally induced mechanical vibrations and a high per-chip cost as well as a large overall instrument footprint in mobile applications. The initial motivation of this work is therefore to develop a cost-effective and robust bacterial detecting device based on an electrochemical technique.…”

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confidence: 99%

Voltammetric Detection and Profiling of Isoprenoid Quinones Hydrophobically Transferred From Bacterial Cells

et al. 2015

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We have developed a novel bacterial detection technique by desiccating a bacterial suspension deposited on an electrode. It was also found that the use of an indium-tin-oxide (ITO) electrode dramatically improved the resolution of the voltammogram, allowing us to observe two pairs of redox peaks, each assigned to the adsorption of isoprenoid ubiquinone (UQn) and menaquinone (MKn), which were present in the bacterial cell envelopes, giving midpeak potentials of -0.015 and -0.25 V versus Ag|AgCl|saturated KCl| at pH 7.0, respectively. Most of the microorganisms classified in both the Gram-negative and -positive bacteria gave well-defined redox peaks, demonstrating that this procedure made the detection of the quinones possible without solvent extraction. It has been demonstrated that the present technique can be used not only for the detection of bacteria, but also for profiling of the isoprenoid quinones, which play important roles in electron and proton transfer in microorganisms. In this respect, the present technique provides a much more straightforward way than the solvent extraction in that one sample can be prepared in 1 min by heat evaporation of a suspension containing the targeted bacteria, which has been applied on the ITO electrode.

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confidence: 99%

Voltammetric Detection and Profiling of Isoprenoid Quinones Hydrophobically Transferred From Bacterial Cells

et al. 2015

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“…A broad anodic peak at 0.95 V (the inset) and at around 190 s (a), appearing only in the first sweep of the cyclic voltammetric experiment, indicates a response typical of PPy overoxidation. 46,[93][94][95][96][97] As shown in Fig. 4b, a slight increase (700 Hz) at 190 s and a subsequent large increase (2700 Hz) at 230 s were observed in the resonance frequency, while the resonance resistance sharply dropped at 230 s, suggesting curing of the film (Fig.…”

Section: Molecularly Imprinted Electrodementioning

confidence: 80%

Placement of Nanospace on an Electrode for Biosensing

et al. 2012

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Electrical and electrochemical methods are well established as very useful techniques in the field of biosensing because they can easily handle signals and devices. This paper provides an overview of biosensing using a nanometer-sized space functionally. Placed effectively on the electrode, the nanospace offers several advantages, such as increased sensitivity, improved selectivity, decreased response time, and the potential for instrument miniaturization. Given the impressive technological progress of nanospace biosensors and its growing impact on analytical science, this review offers an easyto-understand presentation describing the history, recent advances, new methods, and future prospects of nanospace biosensors.

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“…A wide variety of functional monomers such as those forming pyrrole conducting polymers [30,31,32], polyurethane layers [33,34], self-assembled monolayers [35,36] and sol-gels [37,38] have been used in the design of imprints against microorganisms [12]. The imprinting of whole microorganism were successfully achieved by creating selective recognition cavities [12,39] both on polymeric beads [40,41,42] and in polymeric films via stamping method [34,43].…”

Section: Imprinting Of Microorganismsmentioning

confidence: 99%

“…Apart from functional monomers such as acrylic acid and sol-gel based ones, monomers used to form conducting polymers have found wide application in order to imprint biomolecules including microorganisms [31,32,64]. The properties of easy preparation and high conductivity makes these polymers appropriate for designing biosensors.…”

Section: Applicationsmentioning

confidence: 99%

Imprinting of Microorganisms for Biosensor Applications

İdil

Mattìasson

2017

Sensors

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There is a growing need for selective recognition of microorganisms in complex samples due to the rapidly emerging importance of detecting them in various matrices. Most of the conventional methods used to identify microorganisms are time-consuming, laborious and expensive. In recent years, many efforts have been put forth to develop alternative methods for the detection of microorganisms. These methods include use of various components such as silica nanoparticles, microfluidics, liquid crystals, carbon nanotubes which could be integrated with sensor technology in order to detect microorganisms. In many of these publications antibodies were used as recognition elements by means of specific interactions between the target cell and the binding site of the antibody for the purpose of cell recognition and detection. Even though natural antibodies have high selectivity and sensitivity, they have limited stability and tend to denature in conditions outside the physiological range. Among different approaches, biomimetic materials having superior properties have been used in creating artificial systems. Molecular imprinting is a well suited technique serving the purpose to develop highly selective sensing devices. Molecularly imprinted polymers defined as artificial recognition elements are of growing interest for applications in several sectors of life science involving the investigations on detecting molecules of specific interest. These polymers have attractive properties such as high bio-recognition capability, mechanical and chemical stability, easy preparation and low cost which make them superior over natural recognition reagents. This review summarizes the recent advances in the detection and quantification of microorganisms by emphasizing the molecular imprinting technology and its applications in the development of sensor strategies.

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Vertical Immobilization of Viable Bacilliform Bacteria into Polypyrrole Films

Cited by 29 publications

References 18 publications

Voltammetric Detection and Profiling of Isoprenoid Quinones Hydrophobically Transferred From Bacterial Cells

Voltammetric Detection and Profiling of Isoprenoid Quinones Hydrophobically Transferred From Bacterial Cells

Placement of Nanospace on an Electrode for Biosensing

Imprinting of Microorganisms for Biosensor Applications

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