A closed system for plant production with artificial light is an innovative method of plant cultivation. The objective of this study was to investigate the effect of light colour on rooting cuttings and subsequent growth of chrysanthemum (Chrysanthemum × grandiflorum Ramat./Kitam.) During the experiments, the following conditions were maintained: photoperiod 16 h or 10 h, temperature 22 °C, relative humidity of 65–70%. LED lamps emitted the following light colours: white, blue, white + blue (50:50), and red + blue (75:25). For all light spectra, the photosynthetic photon flux density (PPFD) was 50 μmol m−2 s−1. The effectiveness of exposure to different light colours was measured with parameters: cutting weight (g), cutting length (cm), length of roots, and index of leaf greenness (SPAD). The measurements referred to plant features determining plant quality, i.e., the number of flower buds and flower head, the diameter of the flower head, height of plants, index of leaf greenness (SPAD), the number of leaves, and the fresh and dry weights of aboveground parts of plants. The rooting of cuttings and subsequent growth are integral processes in the cultivation of potted chrysanthemums. Both were differently affected by the colour of light from LED lamps. The exposure to red + blue light resulted in the highest leaf greenness index (SPAD) value and the shortest cuttings with the longest roots. White + blue light significantly influenced most of the growth parameters, except the height of the plants and the number of leaves.