Vertical transmission and prevalence of white spot syndrome virus (WSSV) in the wild spawning population of the Indian white shrimp, Penaeus indicus

Vijayan, K.K.; Shyne Anand, P.S.; Balasubramanian, C.P.; Sahaya Rajan, Joseph; Ezhil Praveena, P.; Aravind, R.; Sudheer, N.S.; Francis, Biju; Panigrahi, A.; Otta, S.K.

doi:10.1016/j.jip.2024.108058

Cited by 3 publications

(2 citation statements)

References 38 publications

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“…Ideally, this type of research should involve a combination of individual and group infection experiments to determine the relation between the disease progress in an individual host and the transmission in a population [221][222][223]. Since WSSV reportedly uses more than one infection route (e.g., water-borne [88,101], peroral [216,224], and transovarial [225,226]), each single aspect of the set-up must be considered very carefully to avoid drawing erroneous conclusions when analyzing the results [221][222][223]. For example, the results of a recent study by Cox et al [102] in L. vannamei showed that individually housed WSSV-infected shrimp started shedding viral DNA in the water within 6 h of clinical disease onset and this shedding reached a peak around the time of death.…”

Section: Studies On Transmission Dynamics and Epidemiologymentioning

confidence: 99%

“…Estimation of genetic parameters for WSD resistance/susceptibility can be conducted in an individual WSSV challenge model, perhaps to subsequently estimate the degree of genomic relationship between test animals and individual candidate breeding animals within families to estimate their individual genomic breeding values [213]. After all, the test animals themselves are rarely incorporated in breeding programs, because they might vertically transmit the virus to their offspring [213,226]. Selection for host infectivity, on the other hand, requires that animals are maintained in groups in an environment that encourages reproduction of WSD, and individual inoculation is of lesser importance [213] (Table 5).…”

Section: Genetic Selectionmentioning

confidence: 99%

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Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp

Cox,

De Swaef,

Corteel

et al. 2024

Viruses

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White spot syndrome virus (WSSV) is marked as one of the most economically devastating pathogens in shrimp aquaculture worldwide. Infection of cultured shrimp can lead to mass mortality (up to 100%). Although progress has been made, our understanding of WSSV’s infection process and the virus–host–environment interaction is far from complete. This in turn hinders the development of effective mitigation strategies against WSSV. Infection models occupy a crucial first step in the research flow that tries to elucidate the infectious disease process to develop new antiviral treatments. Moreover, since the establishment of continuous shrimp cell lines is a work in progress, the development and use of standardized in vivo infection models that reflect the host–pathogen interaction in shrimp is a necessity. This review critically examines key aspects of in vivo WSSV infection model development that are often overlooked, such as standardization, (post)larval quality, inoculum type and choice of inoculation procedure, housing conditions, and shrimp welfare considerations. Furthermore, the usefulness of experimental infection models for different lines of WSSV research will be discussed with the aim to aid researchers when choosing a suitable model for their research needs.

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Section: Studies On Transmission Dynamics and Epidemiologymentioning

confidence: 99%

Section: Genetic Selectionmentioning

confidence: 99%

Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp

Cox,

De Swaef,

Corteel

et al. 2024

Viruses

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Reproductive and nursery performance of Kuruma shrimp Penaeus (Marsupenaeus) japonicus Form II: effect of sandy bottom and light intensity in the rearing system

Anand,

Aravind,

Balasubramanian

et al. 2024

Aquacult Int

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Multiplex CRISPR‐Cas Assay for Rapid, Isothermal and Visual Detection of White Spot Syndrome Virus (WSSV) and Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimp

Kanitchinda,

Sritunyalucksana,

Chaijarasphong

2024

Journal of Fish Diseases

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White spot syndrome virus (WSSV) and Enterocytozoon hepatopenaei (EHP) represent the most economically destructive pathogens in the current shrimp industry. WSSV causes white spot disease (WSD) responsible for rapid shrimp mortality, while EHP stunts growth and therefore reduces overall productivity. Despite the importance of timely disease detection, current diagnostic methods for WSSV and EHP are typically singleplex, and those offering multiplex detection face issues such as complexity, low field compatibility and/or low sensitivity. Here, we introduce an orthogonal, multiplex CRISPR‐Cas assay for concomitant detection of WSSV and EHP. This method combines recombinase polymerase amplification (RPA) for target DNA enrichment with Cas12a and Cas13a enzymes for fluorescent detection. This assay produces distinct fluorescent colours for different diagnostic outcomes, allowing naked eye visualisation without ambiguity. Further validation reveals that the assay detects as few as 20 and 200 copies of target DNA from EHP and WSSV, respectively, while producing no false positives with DNA from other shrimp pathogens. Moreover, the assay excellently agrees with established PCR methods in evaluation of clinical samples. Requiring only 37°C and less than an hour to complete, multiplex CRISPR‐Cas assay presents a promising tool for onsite diagnostics, offering high accuracy while saving time and resources.

show abstract

Vertical transmission and prevalence of white spot syndrome virus (WSSV) in the wild spawning population of the Indian white shrimp, Penaeus indicus

Cited by 3 publications

References 38 publications

Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp

Experimental Infection Models and Their Usefulness for White Spot Syndrome Virus (WSSV) Research in Shrimp

Reproductive and nursery performance of Kuruma shrimp Penaeus (Marsupenaeus) japonicus Form II: effect of sandy bottom and light intensity in the rearing system

Multiplex CRISPR‐Cas Assay for Rapid, Isothermal and Visual Detection of White Spot Syndrome Virus (WSSV) and Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimp

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