Vertical transmission ofBorrelia turicatae(Spirochaetales:Borreliaceae) by autogenously reproducingOrnithodoros turicata(Ixodida: Argasidae) female naturally infected with the spirochetes

Abstract

Ornithodoros turicata is a vector of relapsing fever (RF) spirochetes in North America and transmits Borrelia turicatae to a variety of vertebrate hosts. The remarkably long lifespan of O. turicata and its ability to maintain spirochetes horizontally (between life stages) and vertically to progeny promotes the perpetuation of B. turicatae in nature. Nevertheless, the reproductive biology of O. turicata is poorly understood. In this report, we collected ticks from a park within a neighborhood of Austin, Texas. They were reared to adulthood and male ticks were individually housed with females. We observed autogenous reproduction by the ticks and further investigated vertical transmission of B. turicatae by quantifying filial infection rates in a cohort of progeny ticks. These results indicate that O. turicata transovarially transmits B. turicatae during autogenous reproduction and further signify the tick as a natural reservoir of the spirochetes.