Verticillium dahliae toxins-induced nitric oxide production in Arabidopsis is major dependent on nitrate reductase

Shi, Fumei; Li, Ying‐Zhang

doi:10.5483/bmbrep.2008.41.1.079

Cited by 66 publications

(41 citation statements)

References 41 publications

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“…tabaci the NR NO-generated mechanism increased plant resistance. A similar trend of response was observed in Verticillium dahliae infection in arabidopsis (Shi and Li, 2008). Yamamoto-Katou et al (2006) showed that silencing NR leads to reduced levels of NO in response to elicitin.…”

Section: Nitric Oxide In Plant Defencesupporting

confidence: 70%

Moving nitrogen to the centre of plant defence against pathogens

Mur

Simpson

Kumari

et al. 2016

Ann Bot

157

193

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Section: Nitric Oxide In Plant Defencesupporting

confidence: 70%

Moving nitrogen to the centre of plant defence against pathogens

Mur

Simpson

Kumari

et al. 2016

Ann Bot

157

193

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“…Moreover, although there is an abundant literature documenting the lower accumulation and/or detection of NO in Atnoa1 (20,22,24,79,80), there are also reports showing that Atnoa1 is not always impaired in NO accumulation. For example, in response to H 2 O 2 , to iron, to indole 3-butyric acid, to Verticillium dahliae toxins, or to zeatin, NO production is as high in Atnoa1 as in wild type plants (23,41,42,81,82). The presence of a nitrite-dependent NO production pathway in Atnoa1 might account for those conflicting observations.…”

Section: Tablementioning

confidence: 99%

“…This led to the renaming of AtNOS1 as NO-associated protein 1 (AtNOA1) (25). Nevertheless, publications still refer to AtNOS/A1 as a potential NOS (41,42). Here, we examined the ability of AtNOS1 protein to bind and oxidize arginine into NO, using several independent assays.…”

mentioning

confidence: 99%

AtNOS/AtNOA1 Is a Functional Arabidopsis thaliana cGTPase and Not a Nitric-oxide Synthase

Moreau

Lee

Wang

et al. 2008

Journal of Biological Chemistry

280

224

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AtNOS1 was previously identified as a potential nitric-oxide synthase (NOS) in Arabidopsis thaliana, despite lack of sequence similarity to animal NOSs. Although the dwarf and yellowish leaf phenotype of Atnos1 knock-out mutant plants can be rescued by treatment with exogenous NO, doubts have recently been raised as to whether AtNOS1 is a true NOS. Moreover, depending on the type of physiological responses studied, Atnos1 is not always deficient in NO induction and/or detection, as previously reported. Here, we present experimental evidence showing that AtNOS1 is unable to bind and oxidize arginine to NO. These results support the argument that AtNOS1 is not a NOS. We also show that the renamed NO-associated protein 1 (AtNOA1) is a member of the circularly permuted GTPase family (cGTPase). AtNOA1 specifically binds GTP and hydrolyzes it. Complementation experiments of Atnoa1 mutant plants with different constructs of AtNOA1 show that GTP hydrolysis is necessary but not sufficient for the physiological function of AtNOA1. Mutant AtNOA1 lacking the C-terminal domain, although retaining GTPase activity, failed to complement Atnoa1, suggesting that this domain plays a crucial role in planta. cGTPases appear to be RNA-binding proteins, and the closest homolog of AtNOA1, the Bacillus subtilis YqeH, has been shown to participate in ribosome assembly and stability. We propose a similar function for AtNOA1 and discuss it in the light of its potential role in NO accumulation and plant development.Numerous studies have demonstrated that plants, like animals, generate nitric oxide (NO) 3 to regulate a wide range of physiological processes. NO is involved in plant development; it represses flowering (1), reduces seed dormancy (2), and regulates germination (3). NO production has also been detected following different environmental stresses. For example, NO regulates stomata closure in response to abiotic stress (4), and in response to biotic stress, NO participates in induction of plant defenses (5-7).Although NO plays a role as significant in plants as it does in animals, NO synthesis in planta is still a matter of debate (8). Two major routes have been proposed for NO formation in plants. The first one relies on the reduction of nitrite to NO. Several studies demonstrate that nitrate reductase, whose primary function is to catalyze the reduction of nitrate to nitrite, can convert nitrite to NO with low efficiency (9, 10). Nitrite can also be reduced to NO by a plasma membrane-bound nitrite:NOreductase(11),byamitochondrialelectrontransportdependent reductase (12), or nonenzymatically in acidic, reducing environments (13). The second probable NO biosynthetic pathway uses arginine as a substrate, following a reaction similar to that observed for the well characterized animal NOSs. Indeed, several lines of evidence suggest the existence of a mammalian NOS-like enzyme in plants. Application of arginine analogs, inhibitors of animal NOSs, results in a reduction of NO detected in plants (5, 6, 14 -17). Arginine-dependent citrul...

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“…20 The wild-type and nia1, nia2 NR-deficient mutants of Arabidopsis were used to visualize microtubules in living leaf cells. The results showed that VD-toxins induced a timedependent microtubule depolymerization, and that microtubule depolymerization was more severe in WT than in nia1, nia2 NRdeficient mutants, especially at the later stages (Fig.…”

Section: No and H 2 O 2 Modulates Vd-toxins-induced Dynamic Microtubumentioning

confidence: 99%

NO serves as a signaling intermediate downstream of H₂O₂to modulate dynamic microtubule cytoskeleton during responses to VD-toxins in Arabidopsis

Yao

Pei

Zhou

et al. 2012

Plant Signaling & Behavior

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Verticillium dahliae toxins-induced nitric oxide production in Arabidopsis is major dependent on nitrate reductase

Cited by 66 publications

References 41 publications

Moving nitrogen to the centre of plant defence against pathogens

Moving nitrogen to the centre of plant defence against pathogens

AtNOS/AtNOA1 Is a Functional Arabidopsis thaliana cGTPase and Not a Nitric-oxide Synthase

NO serves as a signaling intermediate downstream of H₂O₂to modulate dynamic microtubule cytoskeleton during responses to VD-toxins in Arabidopsis

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Verticillium dahliae toxins-induced nitric oxide production in Arabidopsis is major dependent on nitrate reductase

Cited by 66 publications

References 41 publications

Moving nitrogen to the centre of plant defence against pathogens

Moving nitrogen to the centre of plant defence against pathogens

AtNOS/AtNOA1 Is a Functional Arabidopsis thaliana cGTPase and Not a Nitric-oxide Synthase

NO serves as a signaling intermediate downstream of H2O2to modulate dynamic microtubule cytoskeleton during responses to VD-toxins in Arabidopsis

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NO serves as a signaling intermediate downstream of H₂O₂to modulate dynamic microtubule cytoskeleton during responses to VD-toxins in Arabidopsis