Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

Usami, Toshiyuki; Kanto, Takeshi; Inderbitzin, Patrik; Itoh, Mizuho; Kisaki, Gan; Ebihara, Yoshiyuki; Suda, Wataru; Amemiya, Yoshimiki; Subbarao, Krishna V.

doi:10.1007/s10327-010-0282-x

Cited by 16 publications

(10 citation statements)

References 17 publications

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“…Furthermore, none of the isolates could be recovered from the petioles of plants inoculated with these isolates. Although V. tricorpus and G. nigrescens have generally been reported as weak pathogens on some plant species ( (34,(36)(37)(38)74,75), pathogenicity of isolates of these species was not observed in this study. Nonpathogenic isolates of V. tricorpus on Chinese cabbage, cotton, eggplant, green pepper, larkspur, parsley, snapdragon, soybean, tobacco, and tomato (31,34,65) have been documented, as well as nonpathogenic isolates of G. nigrescens on cantaloupe, eggplant, peppermint, and tomato (65).…”

Section: Discussioncontrasting

confidence: 55%

Pathogenicity, Virulence, and Vegetative Compatibility Grouping of Verticillium Isolates from Spinach Seed

et al. 2013

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Iglesias-Garcia, A. M., Villarroel-Zeballos, M. I., Feng, C, du Toit, L. J., and Correll, J. C. 2013. Pathogenicity, virulence, and vegetative compatibility grouping of Verticillium isolates from spinach seed. Plant Dis. 97:1457-1469.In 2005, Verticillium dahliae was first reported to be pathogenic to spinach seed crops in the Pacific Northwest, with symptoms only developing after initiation of the reproductive stage of plant growth, and to be prevalent on commercial spinach seed lots produced in Denmark, The Netherlands, and the United States. In this study, the genetic diversity, pathogenicity, and virulence were examined for a collection of isolates of Verticillium spp. from spinach as well as other hosts (alfalfa, cotton, lettuce, mint, peppermint, potato, radish, and tomato) from various countries and from different vegetative compatibility groups (VCGs). Of a total of 210 isolates of V. dahliae obtained from spinach seed produced in Denmark, the Netherlands, New Zealand, or the United States, 128 were assigned to VCG 4B (89% of 91 U.S. isolates, 86% of 42 isolates from the Netherlands, 19% of 43 Denmark isolates, and 8% of 13 New Zealand isolates), 65 to VCG 2B (92% of the New Zealand isolates, 79% ofthe Denmark isolates, 14% ofthe Netherlands isolates, and 9% of the U.S. isolates), and 3 to VCG 2A (2% of each of the Denmark and U.S. isolates, and 0% of the Netherlands and New Zealand isolates); 14 isolates could not be assigned to a VCG. Although little variation in the sequence of the internal transcribed spacer (ITS) region of ribosomal DNA was observed among isolates within each Verticillium sp., the ITS region readily differentiated isolates of the species V. dahliae, V. tricorpus, and Gibellulopsis nigrescens (formerly V. nigrescens) obtained from spinach seed. Greenhouse pathogenicity assays on spinach, cotton, lettuce, and tomato plants using isolates of V. dahliae (n = 29 to 34 isolates), V. tricorpus {n = 3), G. nigrescens (n = 2), and V albo-atrum (n = 1) originally obtained from these hosts as well as from alfalfa, mint, peppermint, potato, and radish, revealed a wide range in virulence among the isolates. Isolates of V. tricorpus and G. nigrescens recovered from spinach seed and an isolate of V. albo-atrum from alfalfa were not pathogenic on spinach. In addition, isolates of V. dahliae from mint and peppermint were not pathogenic or only weakly virulent on the hosts evaluated. Although there was a wide range in virulence among the isolates of V. dahliae tested, all of the V. dahliae isolates caused Verticillium wilt symptoms on spinach, lettuce, tomato, and cotton. None of the isolates of V. dahliae showed host specificity. These results indicate that Verticillium and related species associated with spinach seed display substantial variability in virulence and pathogenicity to spinach and other plants but the V. dahliae isolates were restricted to three VCGs.

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Section: Discussioncontrasting

confidence: 55%

Pathogenicity, Virulence, and Vegetative Compatibility Grouping of Verticillium Isolates from Spinach Seed

et al. 2013

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“…), while others were pathogenic to some hosts (Isaac ; Usami et al . ). The differences in pathogenicity among isolates are difficult to interpret, particularly due to limitations in the taxonomy of Verticillium .…”

Section: Introductionmentioning

confidence: 97%

The endophyteVerticillium Vt305 protects cauliflower against Verticillium wilt

Tyvaert

França

Debode³

et al. 2014

J Appl Microbiol

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Aims: To investigate the interaction between cauliflower and the isolate Verticillium Vt305, obtained from a field suppressive to Verticillium wilt of cauliflower, and to evaluate the ability of Verticillium Vt305 to control Verticillium wilt of cauliflower caused by V. longisporum. Methods and Results: Single and combined inoculations of Verticillium Vt305 and V. longisporum were performed on cauliflower seedlings. Symptom development was evaluated, and fungal colonization was measured in the roots, hypocotyl and stem with real-time PCR. No symptoms were observed after single inoculation of Verticillium Vt305, although it colonized the plant tissues. Pre-inoculation of Verticillium Vt305 reduced symptom development and colonization of plant tissues by V. longisporum. Conclusions: Verticillium Vt305 is an endophyte on cauliflower plants and showed effective biological control of V. longisporum in controlled conditions. Significance and Impact of the Study: This work can contribute to the development of a sustainable control measure of V. longisporum in Brassicaceae hosts, which is currently not available. Additionally, this study provides evidence for the different roles of Verticillium species present in the agro-ecosystem.

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“…Among the ten species currently recognized in Verticillium sensu stricto, [4], [5], V. dahliae is most widespread and most economically important [1], [6], [7], but V. albo-atrum [8], V. alfalfae [9], [10], V. longisporum [11], [12], V. nonalfalfae [13], [14], V. tricorpus [8], [15] and V. zaregamsianum [16] also cause significant losses, V. nubilum causes disease in pathogenicity tests [15], and both V. isaacii and V. klebahnii have been recovered from lettuce and artichoke, respectively [4], [17]. One of the characteristic features of Verticillium species is the formation of resting structures [4].…”

Section: Introductionmentioning

confidence: 99%

Identification and Differentiation of Verticillium Species and V. longisporum Lineages by Simplex and Multiplex PCR Assays

et al. 2013

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Accurate species identification is essential for effective plant disease management, but is challenging in fungi including Verticillium sensu stricto (Ascomycota, Sordariomycetes, Plectosphaerellaceae), a small genus of ten species that includes important plant pathogens. Here we present fifteen PCR assays for the identification of all recognized Verticillium species and the three lineages of the diploid hybrid V. longisporum. The assays were based on DNA sequence data from the ribosomal internal transcribed spacer region, and coding and non-coding regions of actin, elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase and tryptophan synthase genes. The eleven single target (simplex) PCR assays resulted in amplicons of diagnostic size for V. alfalfae, V. albo-atrum, V. dahliae including V. longisporum lineage A1/D3, V. isaacii, V. klebahnii, V. nonalfalfae, V. nubilum, V. tricorpus, V. zaregamsianum, and Species A1 and Species D1, the two undescribed ancestors of V. longisporum. The four multiple target (multiplex) PCR assays simultaneously differentiated the species or lineages within the following four groups: Verticillium albo-atrum, V. alfalfae and V. nonalfalfae; Verticillium dahliae and V. longisporum lineages A1/D1, A1/D2 and A1/D3; Verticillium dahliae including V. longisporum lineage A1/D3, V. isaacii, V. klebahnii and V. tricorpus; Verticillium isaacii, V. klebahnii and V. tricorpus. Since V. dahliae is a parent of two of the three lineages of the diploid hybrid V. longisporum, no simplex PCR assay is able to differentiate V. dahliae from all V. longisporum lineages. PCR assays were tested with fungal DNA extracts from pure cultures, and were not evaluated for detection and quantification of Verticillium species from plant or soil samples. The DNA sequence alignments are provided and can be used for the design of additional primers.

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Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

Cited by 16 publications

References 17 publications

Pathogenicity, Virulence, and Vegetative Compatibility Grouping of Verticillium Isolates from Spinach Seed

Pathogenicity, Virulence, and Vegetative Compatibility Grouping of Verticillium Isolates from Spinach Seed

The endophyteVerticillium Vt305 protects cauliflower against Verticillium wilt

Identification and Differentiation of Verticillium Species and V. longisporum Lineages by Simplex and Multiplex PCR Assays

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