Very high Gravity (VHG) Bioethanol Production Using Modified Simultaneous Saccharification and Fermentation of Raw Cassava Chips with Molasses by Kluyveromyces marxianus DMKU-KS07

Kitpreechavanich, Vichien; Netprasom, Pornnapa; Kancharu, Netnapha; Jitmala, Kanokwan; Areesirisuk, Atsadawut; Trakarnpaiboon, Srisakul

doi:10.1007/s12649-020-01257-1

Cited by 14 publications

(12 citation statements)

References 34 publications

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“…All statistical analyses were analyzed using SPSS for Windows version 20 (SPSS Inc., USA). Differences among the mean values were tested using the least significant difference multiple range test, and significance was taken at the p < 0.05 level [5,16].…”

Section: Discussionmentioning

confidence: 99%

“…Glucose, maltose, and maltotriose were used as standards. The morphological changes in native and digested broken Riceberry rice powder before and after ultrasound-assisted enzymatic hydrolysis were investigated using scanning electron microscopy (SEM) (Model SU8020; Hitachi, Tokyo, Japan) as described by Lomthong et al [16].…”

Section: Ultrasound-assisted Enzymatic Hydrolysis Of Broken Riceberry...mentioning

confidence: 99%

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Ultrasound-assisted enzymatic hydrolysis of broken Riceberry rice for sugar syrup production as a substrate for bacterial cellulose facial mask development

Kitpreechavanich¹,

Siripornvisal²,

Khunnamwong³

2022

J Appl Biol Biotech

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Broken Riceberry rice powder was saccharified by ultrasound-assisted enzymatic hydrolysis for the production of sugar syrup containing phenolic compounds. The results showed that total soluble solid (TSS) content at 17.0 ± 0.5°Brix was obtained when sonicating broken Riceberry rice powder (250 g/l) with a low-temperature amylase at 60% amplitude for 20 minutes and subsequent hydrolysis at 50 o C for 12 hours. Upscaled hydrolysis in a 5.0 l reactor gave the highest TSS content and total phenolic content (TPC) at 16.33 ± 0.29°Brix and 5.63 ± 0.12 mg GAE/g sample, respectively. The obtained Riceberry syrup was used for bacterial cellulose (BC) fermentation using Komagataeibacter xylinus AGR 60, which was incubated at room temperature for 4 days and yielded 4.80 ± 0.30 mm of thickness with 2.51 ± 0.24 g of dry weight equivalent to 3.14 ± 0.30 g/l/day. The physical structure of the obtained BC showed a crowded cellulose network, which showed the potential for water holding capacity of a facial mask sheet product. The five facial mask formulations were tested and showed significant results for the storage ability. This study provided an innovative product from the low-cost agricultural crop through the biotechnological process and showed the potential for further application at the commercial level. How to cite this article:Lomthong T, Siripornvisal S, Khunnamwong P. Ultrasoundassisted enzymatic hydrolysis of broken Riceberry rice for sugar syrup production as a substrate for bacterial cellulose facial mask development.

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Section: Discussionmentioning

confidence: 99%

Section: Ultrasound-assisted Enzymatic Hydrolysis Of Broken Riceberry...mentioning

confidence: 99%

Ultrasound-assisted enzymatic hydrolysis of broken Riceberry rice for sugar syrup production as a substrate for bacterial cellulose facial mask development

Kitpreechavanich¹,

Siripornvisal²,

Khunnamwong³

2022

J Appl Biol Biotech

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“…A yeast strain of Kluyveromyces marxianus DMKU-KS07 was kindly provided from the yeast culture collection of Prof. Dr. Savitree Limtong, Department of Microbiology, Faculty of Science, Kasetsart University. The yeast inoculum was prepared according to the method described by Lomthong et al [12] For expression of the recombinant LsA175, the culture process was conducted following the method of Lomthong et al [3] A single colony of E. coli BL21 (DE3) harboring recombinant plasmid was cultured in 5.0 mL of LB media supplemented with 100 μg mL −1 ampicillin and incubated at 37 °C in a shaking incubator for 12 h. The obtained culture was transferred into 50 mL of basal medium, which consisted of 5.0 g L −1 glucose, 11.9 g L −1 K 2 HPO 4 , 2.4 g L −1 KH 2 PO 4 , 1.8 g L −1 NaCl, 3.0 g L −1 (NH 4 ) 2 SO 4 , 0.11 g L −1 , and MgSO 4 7H 2 O supplemented with 100 μg mL −1 ampicillin. [13] The recombinant cells were grown until the optical density at 600 nm up to 2.0, reaching the midlogarithmic phase.…”

Section: Microorganisms Inoculum Preparation and Cultivationmentioning

confidence: 99%

“…The sterile nutrient solution was added to obtain a final concentration of 6.0 g L −1 yeast extract, 4.0 g L −1 (NH 4 ) 2 SO 4 , 2.0 g L −1 MgSO 4 , and 8.0 g L −1 of KH 2 PO 4 , with an initial pH of 5.0. [6,12] Then, the K. marxianus DMKU-KS07 at 10% (v/v) was inoculated into the medium. The fermentation was incubated at 42 °C in a rotary shaking incubator at 300 rpm for 36 h. [6] A sample from the process was taken every 6 h to determine total sugars, reducing sugars, and ethanol concentrations.…”

Section: Bioethanol Fermentationmentioning

confidence: 99%

“…[30] This study used the thermotolerant yeast strain, K. marxianus DMKU-KS07, in a modified SSF process as described in the method. [6,12] The broken rice powder was hydrolyzed at optimum conditions with released reducing sugar content at 89.0 ± 3.22 g L −1 after incubation at 50 °C for 12 h. The enzymatic hydrolysate of broken rice, which was further subjected to ethanol fermentation by K. marxianus DMKU-KS07 at 42 °C, yielded 71.4±4.35 g L −1 of ethanol as shown in Figure 5. However, the total sugar of 42.8±5.5 g L −1 remained in the fermentation as non-digested residues of broken rice powder and non-fermentable maltooligosaccharide.…”

Section: Bioethanol Fermentationmentioning

confidence: 99%

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Potential of Recombinant Raw Starch‐Degrading Enzyme from Escherichia coli for Sugar Syrup and Bioethanol Productions Using Broken Rice Powder as Substrate

et al. 2021

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The recombinant raw starch-degrading enzyme (LsA175) which harbors the lsa175 gene from Laceyella sacchari LP175 is optimized for expression in Escherichia coli BL21 (DE3). The recombinant strain provides an alternative avenue for enzyme production in a stirrer fermenter with tolerance to shear force and avoids losing stability by protease existing in the native strain. The maximum enzyme production at 4472±112 U g −1 cell dry weight is obtained using the optimum conditions at 22 °C, 1.0 mM IPTG, and 24 h of induction temperature, Isopropyl 𝜷d-1-thiogalactopyranoside (IPTG) concentration, and induction time, respectively. The enzyme production of 4545 ± 89.8 U g −1 cell dry weight is obtained by batch fermentation in a 2.0 L stirrer fermenter and 5329 ± 86.1 U g −1 cell dry weight by continued glucose feeding with high bacterial cell concentration. The uncooked broken rice powder (200 g L −1 ) is hydrolyzed by crude recombinant LsA175 (300 U mL −1 ) at 50 °C for 12 h without the addition of exogenous glucoamylase (GA), yielding 86 ± 1.79 g L −1 reducing sugar. The bioethanol production resulting from modified simultaneous saccharification and fermentation with Kluyveromyces marxianus DMKU-KS07 at 42 °C gave 71.4±4.35 g L −1 ethanol with 88.24% of theoretical ethanol yield. LsA175 presents engaging performances regarding sugar syrup and bioethanol production, which can diminish the cost of production and energy consumption.

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Raw starch degrading alkaline α‐amylase from Geobacillus kaustophilus TSCCA02: Production, characterization, and its potential for application as a detergent additive

Phonlamai,

Kingkaew,

Prajanket

et al. 2024

J Basic Microbiol

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Geobacillus kaustophilus TSCCA02, a newly isolated strain from cassava (Manihot esculenta L.) rhizosphere soil in Thailand, showed maximum raw starch degrading enzyme (RSDE) activity at 252.3 ± 9.32 U/mL with cassava starch and peptone at 5.0 and 3.0 g/L, respectively. 16 S ribosomal RNA (rRNA) sequencing and phylogenetic tree analyses indicated that the TSCCA02 strain was closely related to G. kaustophilus. The crude RSDE had optimal activity at 60°C and pH 9.0. This enzyme degraded various kinds of starch including potato starch, cassava starch, rice flour, corn starch, glutinous rice flour, and wheat flour to produce sugar syrup at 60°C, as confirmed by scanning electron microscopy (SEM), thin‐layer chromatography (TLC), and Fourier‐transform infrared spectroscopy (FTIR). The major end products of starch hydrolysis were maltose and maltotriose with a small amount of glucose, confirming this enzyme as an α‐amylase. The enzyme improved the washing efficiency of cotton fabric with commercial detergent. Results indicated the potential of alkaline α‐amylase produced from a new isolate of G. kaustophilus TSCCA02 for application as a detergent additive on an industrial scale.

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Very high Gravity (VHG) Bioethanol Production Using Modified Simultaneous Saccharification and Fermentation of Raw Cassava Chips with Molasses by Kluyveromyces marxianus DMKU-KS07

Cited by 14 publications

References 34 publications

Ultrasound-assisted enzymatic hydrolysis of broken Riceberry rice for sugar syrup production as a substrate for bacterial cellulose facial mask development

Ultrasound-assisted enzymatic hydrolysis of broken Riceberry rice for sugar syrup production as a substrate for bacterial cellulose facial mask development

Potential of Recombinant Raw Starch‐Degrading Enzyme from Escherichia coli for Sugar Syrup and Bioethanol Productions Using Broken Rice Powder as Substrate

Raw starch degrading alkaline α‐amylase from Geobacillus kaustophilus TSCCA02: Production, characterization, and its potential for application as a detergent additive

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