Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment

Eljaszewicz, Andrzej; Bołkuń, Łukasz; Grubczak, Kamil; Rusak, Małgorzata; Wasiluk, Tomasz; Dąbrowska, Milena; Radziwon, Piotr; Marlicz, Wojciech; Kamiński, Karol; Kłoczko, Janusz; Moniuszko, Marcin

doi:10.1155/2018/1943980

Cited by 12 publications

(8 citation statements)

References 40 publications

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“…In contrast to healthy blood donors, patients with ALL had remarkably lower levels of CD14+ monocytes following chemotherapy. Moreover, in the patients who were G‐CSF treated, significantly more often intermediate monocytes and less often classical monocytes were observed 102 …”

Section: Quantitative Dependencies Between Monocyte Subsets and Theirmentioning

confidence: 97%

“…Moreover, in the patients who were G-CSF treated, significantly more often intermediate monocytes and less often classical monocytes were observed. 102 An increased percentage of classical monocytes (>94%) has been found in the peripheral blood of chronic myelomonocytic leukaemia (CMML) patients, a fact which significantly distinguishes CMML from other forms of monocytosis. Furthermore, in CMML patients who respond to hypomethylating agents, the levels of classical monocytes were lower.…”

Section: Haematological Malignancies and Bone Marrow Transplantationmentioning

confidence: 99%

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Pattern of human monocyte subpopulations in health and disease

2020

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Monocytes are important cells of the innate system. They are a heterogeneous type of cells consisting of phenotypically and functionally distinct subpopulations, which play a specific role in the control, development and escalation of the immunological processes. Based on the expression of superficial CD14 and CD16 in flow cytometry, they can be divided into three subsets: classical, intermediate and non‐classical. Variation in the levels of human monocyte subsets in the blood can be observed in patients in numerous pathological states, such as infections, cardiovascular and inflammatory diseases, cancer and autoimmune diseases. The aim of this review is to summarize current knowledge of human monocyte subsets and their significance in homeostasis and in pathological conditions.

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Section: Quantitative Dependencies Between Monocyte Subsets and Theirmentioning

confidence: 97%

Section: Haematological Malignancies and Bone Marrow Transplantationmentioning

confidence: 99%

Pattern of human monocyte subpopulations in health and disease

2020

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“…Immunostained blood cells were fixed with CytoFix. Flow cytometry analysis was performed with the use of a FACSCalibur flow cytometer (BD Immunocytometry Systems, San Jose, CA, USA) as previously described [ 14 , 15 ]. Appropriate fluorescence minus-one (FMO) controls were applied for setting correct compensations and assuring proper gating.…”

Section: Methodsmentioning

confidence: 99%

Monocyte Subsets in Patients with Chronic Heart Failure Treated with Cardiac Resynchronization Therapy

Ptaszyńska‐Kopczyńska

Eljaszewicz

Marcinkiewicz-Siemion

et al. 2021

Cells

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Background: The exact role of individual inflammatory factor in heart failure with reduced ejection fraction (HFrEF) remains elusive. The study aimed to evaluate three monocyte subsets (classical-CD14++CD16−, intermediate-CD14++CD16+, and nonclassical-CD14+CD16++) in HFrEF patients and to assess the effect of the cardiac resynchronization therapy (CRT) on the changes in monocyte compartment. Methods: The study included 85 patients with stable HFrEF. Twenty-five of them underwent CRT device implantation with subsequent 6-month assessment. The control group consisted of 23 volunteers without HFrEF. Results: The analysis revealed that frequencies of non-classical-CD14+CD16++ monocytes were lower in HFrEF patients compared to the control group (6.98 IQR: 4.95–8.65 vs. 8.37 IQR: 6.47–9.94; p = 0.021), while CD14++CD16+ and CD14++CD16− did not differ. The analysis effect of CRT on the frequency of analysed monocyte subsets 6 months after CRT device implantation showed a significant increase in CD14+CD16++ (from 7 IQR: 4.5–8.4 to 7.9 IQR: 6.5–9.5; p = 0.042) and CD14++CD16+ (from 5.1 IQR: 3.7–6.5 to 6.8 IQR: 5.4–7.4; p = 0.017) monocytes, while the frequency of steady-state CD14++CD16− monocytes was decreased (from 81.4 IQR: 78–86.2 to 78.2 IQR: 76.1–81.7; p = 0.003). Conclusions: HFrEF patients present altered monocyte composition. CRT-related changes in the monocyte compartment achieve levels observed in controls without HFrEF.

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“…PerCP, CD16 FITC, HLA-DR APC, CD163 PE (BD Bioscience, information on the antibodies used included in Table 2), according to the stain-and-then-lyse-and-wash protocol, as previously described [26]. Fluorescence-minus-one (FMO) controls were used for setting compensation and to assure correct gating.…”

Section: Blood Collection and Flowmentioning

confidence: 99%

High CD163 Expression on Classical Monocytes Is Associated with Immune Control of HBV Infection in Noncirrhotic Patients

Parfieniuk‐Kowerda

Grubczak

Eljaszewicz

et al. 2020

Mediators of Inflammation

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Background and Aims. The functional impairment of monocytes may contribute to the persistence of HBV infection. This study aims to assess monocyte subpopulations, monocyte expression of CD163, plasma sCD163, and sTWEAK in patients with chronic HBeAg-negative HBV infection at different phases of disease. Methods. Fifty-nine patients with CHB, 9 with a history of HBsAg/anti-HBs seroconversion, were enrolled. The control group consisted of 15 healthy volunteers. Subpopulations of peripheral blood monocytes were distinguished by CD14 and CD16. Membrane expression of CD163 was assessed by flow cytometry, plasma sCD163 concentration by ELISA, and sTWEAK by bead-based multiplexed immunoassay system. Results. CD163 expression was increased in classical and intermediate monocytes in CHB patients and those with HBsAg/anti-HBs seroconversion. CD163 expression on classical monocytes was associated with status of immune control and thus significant in HBV infection as compared to active hepatitis. Plasma sCD163 concentration was increased in CHB patients and those with HBsAg/anti-HBs seroconversion vs. the control group. Positive correlations between plasma sCD163 and ALT, as well as APRI, were observed. Plasma sTWEAK concentration was lower in CHB patients in comparison to patients with HBsAg/anti-HBs seroconversion. Conclusions. Exposure to HBV antigens alters monocyte subsets’ frequencies and activation. The expression of CD163 on classical monocytes increased in parallel with improved immune control of the HBV infection. Patients who seroconverted HBsAg had the highest expression of CD163 on monocytes, which suggests involvement of monocytes in immune control of HBV infection. Persistent inflammation is accompanied by higher CD163 expression and sCD163 level and lower sTWEAK level.

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Very Small Embryonic-Like Stem Cells, Endothelial Progenitor Cells, and Different Monocyte Subsets Are Effectively Mobilized in Acute Lymphoblastic Leukemia Patients after G-CSF Treatment

Cited by 12 publications

References 40 publications

Pattern of human monocyte subpopulations in health and disease

Pattern of human monocyte subpopulations in health and disease

Monocyte Subsets in Patients with Chronic Heart Failure Treated with Cardiac Resynchronization Therapy

High CD163 Expression on Classical Monocytes Is Associated with Immune Control of HBV Infection in Noncirrhotic Patients

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