Vesicle Trafficking during Somatic Cytokinesis

Damme, Daniël Van; Inzé, Dirk; Russinova, Eugenia

doi:10.1104/pp.108.120303

Cited by 32 publications

(26 citation statements)

References 99 publications

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“…Our results show that spatiotemporal localization of AtECA1:sGFP most closely matches that of CLC, at the growing cell plate in particular, which suggests that AtECA1 may function together with CLC at the growing cell plate. Previous studies reported that endocytosis occurs at the growing cell plate (Otegui et al, 2001;Jürgens, 2005;Van Damme et al, 2008;Ito et al, 2012). Furthermore, many proteins involved in CCV-mediated endocytosis have been shown to accumulate in the cell plate during cell division (Otegui et al, 2001;Chow et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Unlike animal cells, which divide by fission, plant cells employ a more complicated mechanism for cytokinesis: a phragmoplast, a cytokinetic organelle, is formed from the remains of spindle microtubules, and a new cell wall is generated at the midplane of the phragmoplast, thereby separating the cytoplasm (Jürgens, 2005). Secretory vesicles originating from the trans-Golgi network (TGN) are delivered to the division plane and fuse to each other via a homotypic fusion to form the cell plate (Mayer and Jürgens, 2004;Jürgens, 2005;Dhonukshe et al, 2006;Chow et al, 2008;Van Damme et al, 2008;Thiele et al, 2009;Reyes et al, 2011). The fused vesicles at the growing cell plate are then further processed via intermediate structures, such as tubulovesicular networks, to form planar fenestrated sheets (Otegui et al, 2001;Jürgens, 2005).…”

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confidence: 99%

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An A/ENTH Domain-Containing Protein Functions as an Adaptor for Clathrin-Coated Vesicles on the Growing Cell Plate in Arabidopsis Root Cells

et al. 2012

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Cytokinesis is the process of partitioning the cytoplasm of a dividing cell, thereby completing mitosis. Cytokinesis in the plant cell is achieved by the formation of a new cell wall between daughter nuclei using components carried in Golgi-derived vesicles that accumulate at the midplane of the phragmoplast and fuse to form the cell plate. Proteins that play major roles in the development of the cell plate in plant cells are not well defined. Here, we report that an AP180 amino-terminal homology/epsin amino-terminal homology domain-containing protein from Arabidopsis (Arabidopsis thaliana) is involved in clathrin-coated vesicle formation from the cell plate. Arabidopsis Epsin-like Clathrin Adaptor1 (AtECA1; At2g01600) and its homologous proteins AtECA2 and AtECA4 localize to the growing cell plate in cells undergoing cytokinesis and also to the plasma membrane and endosomes in nondividing cells. AtECA1 (At2g01600) does not localize to nascent cell plates but localizes at higher levels to expanding cell plates even after the cell plate fuses with the parental plasma membrane. The temporal and spatial localization patterns of AtECA1 overlap most closely with those of the clathrin light chain. In vitro protein interaction assays revealed that AtECA1 binds to the clathrin H chain via its carboxyl-terminal domain. These results suggest that these AP180 amino-terminal homology/epsin amino-terminal homology domain-containing proteins, AtECA1, AtECA2, and AtECA4, may function as adaptors of clathrin-coated vesicles budding from the cell plate.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

An A/ENTH Domain-Containing Protein Functions as an Adaptor for Clathrin-Coated Vesicles on the Growing Cell Plate in Arabidopsis Root Cells

et al. 2012

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“…Cell plate formation is a multistep process that starts during late anaphase and is completed by late telophase (Samuels et al, 1995;Van Damme et al, 2008). Reduction of VLCFA did not affect the targeting of vesicles to the equatorial plane or the first fusion events, which typically take place shortly after to generate the tubulo-vesicular network.…”

Section: Discussionmentioning

confidence: 99%

Very-long-chain fatty acids are required for cell plate formation during cytokinesis inArabidopsis thaliana

Bach

Gissot

Marion

et al. 2011

Journal of Cell Science

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SummaryAcyl chain length is thought to be crucial for biophysical properties of the membrane, in particular during cell division, when active vesicular fusion is necessary. In higher plants, the process of cytokinesis is unique, because the separation of the two daughter cells is carried out by de novo vesicular fusion to generate a laterally expanding cell plate. In Arabidopsis thaliana, very-long-chain fatty acid (VLCFA) depletion caused by a mutation in the microsomal elongase gene PASTICCINO2 (PAS2) or by application of the selective elongase inhibitor flufenacet altered cytokinesis. Cell plate expansion was delayed and the formation of the endomembrane tubular network altered. These defects were associated with specific aggregation of the cell plate markers YFP-Rab-A2a and KNOLLE during cytokinesis. Changes in levels of VLCFA also resulted in modification of endocytosis and sensitivity to brefeldin A. Finally, the cytokinesis impairment in pas2 cells was associated with reduced levels of very long fatty acyl chains in phospholipids. Together, our findings demonstrate that VLCFA-containing lipids are essential for endomembrane dynamics during cytokinesis.

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“…In plant cell division, cytokinesis comprises four main steps: (1) the construction of the phragmoplast, (2) directed transport and fusion of vesicles at the phragmoplast, (3) centrifugal outgrowth of the cell plate, and (4) fortification of the cell plate into a cell wall (Samuels et al, 1995;Assaad, 2001;Van Damme et al, 2008). Several reports have demonstrated that the alteration of one of these processes induces the formation of multinucleate cells, which, upon nuclear fusion, often yield endomitotic polyploid cells (Mayer et al, 1999;Müller et al, 2002;Strompen et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

GLUCAN SYNTHASE-LIKE8 and STEROL METHYLTRANSFERASE2 Are Required for Ploidy Consistency of the Sexual Reproduction System inArabidopsis

et al. 2013

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In sexually reproducing plants, the meiocyte-producing archesporal cell lineage is maintained at the diploid state to consolidate the formation of haploid gametes. In search of molecular factors that regulate this ploidy consistency, we isolated an Arabidopsis thaliana mutant, called enlarged tetrad2 (et2), which produces tetraploid meiocytes through the stochastic occurrence of premeiotic endomitosis. Endomitotic polyploidization events were induced by alterations in cell wall formation, and similar cytokinetic defects were sporadically observed in other tissues, including cotyledons and leaves. ET2 encodes GLUCAN SYNTHASE-LIKE8 (GSL8), a callose synthase that mediates the deposition of callose at developing cell plates, root hairs, and plasmodesmata. Unlike other gsl8 mutants, in which defects in cell plate formation are seedling lethal, cytokinetic defects in et2 predominantly occur in flowers and have little effect on vegetative growth and development. Similarly, mutations in STEROL METHYLTRANSFERASE2 (SMT2), a major sterol biosynthesis enzyme, also lead to weak cytokinetic defects, primarily in the flowers. In addition, SMT2 allelic mutants also generate tetraploid meiocytes through the ectopic induction of premeiotic endomitosis. These observations demonstrate that appropriate callose and sterol biosynthesis are required for maintaining the ploidy level of the premeiotic germ lineage and that subtle defects in cytokinesis may lead to diploid gametes and polyploid offspring.

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Vesicle Trafficking during Somatic Cytokinesis

Cited by 32 publications

References 99 publications

An A/ENTH Domain-Containing Protein Functions as an Adaptor for Clathrin-Coated Vesicles on the Growing Cell Plate in Arabidopsis Root Cells

An A/ENTH Domain-Containing Protein Functions as an Adaptor for Clathrin-Coated Vesicles on the Growing Cell Plate in Arabidopsis Root Cells

Very-long-chain fatty acids are required for cell plate formation during cytokinesis inArabidopsis thaliana

GLUCAN SYNTHASE-LIKE8 and STEROL METHYLTRANSFERASE2 Are Required for Ploidy Consistency of the Sexual Reproduction System inArabidopsis

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