Vesicle trafficking in <i>Arabidopsis</i> pollen tubes

Hao, Guang-Jiu; Zhao, Xin-Ying; Zhang, Miaomiao; Ying, Jun; Yu, F. Richard; Li, Sha; Zhang, Yan

doi:10.1002/1873-3468.14343

Cited by 13 publications

(5 citation statements)

References 138 publications

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“…The defects of both secretion and endocytosis in rha1 ; ara7 pollen tubes would have affected the distribution of membrane proteins whose targeting are dynamically regulated. In addition, wall components, most importantly cellulose and pectin, are delivered to or synthesized at the apex, either directly or indirectly via membrane dynamics at the PM (Cascallares et al ., 2020; Hao et al ., 2022). Both stiffness and flexibility of cell wall are critical for pollen tube growth in vivo (Wang et al ., 2011; Sede et al ., 2018).…”

Section: Resultsmentioning

confidence: 99%

Canonical Rab5 GTPases are essential for pollen tube growth through style in Arabidopsis

Hao

Zhao

et al. 2023

New Phytologist

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Summary Pollen tubes have dynamic tubular vacuoles. Functional loss of AP‐3, a regulator of one vacuolar trafficking route, reduces pollen tube growth. However, the role of canonical Rab5 GTPases that are responsible for two other vacuolar trafficking routes in Arabidopsis pollen tubes is obscure. By using genomic editing, confocal microscopy, pollen tube growth assays, and transmission electron microscopy, we demonstrate that functional loss of canonical Rab5s in Arabidopsis, RHA1 and ARA7, causes the failure of pollen tubes to grow through style and thus impairs male transmission. Functional loss of canonical Rab5s compromises vacuolar trafficking of tonoplast proteins, vacuolar biogenesis, and turgor regulation. However, rha1;ara7 pollen tubes are comparable to those of wild‐type in growing through narrow passages by microfluidic assays. We demonstrate that functional loss of canonical Rab5s compromises endocytic and secretory trafficking at the plasma membrane (PM), whereas the targeting of PM‐associated ATPases is largely unaffected. Despite that, rha1;ara7 pollen tubes contain a reduced cytosolic pH and disrupted actin microfilaments, correlating with the mis‐targeting of vacuolar ATPases (VHA). These results imply a key role of vacuoles in maintaining cytoplasmic proton homeostasis and in pollen tube penetrative growth through style.

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Section: Resultsmentioning

confidence: 99%

Canonical Rab5 GTPases are essential for pollen tube growth through style in Arabidopsis

Hao

Zhao

et al. 2023

New Phytologist

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“…In pollen tubes, the specialized haploid cell responsible for tip growth relies on dynamic vesicular trafficking. However, because of their functional importance, mutations in these regulators can lead to lethality, hampering in-depth mechanistic analysis 56 .…”

Section: Discussionmentioning

confidence: 99%

Vesicle-Mediated Regulation of Pollen tube Growth inTorenia fournieri

Jin,

Mizukami,

Okuda

et al. 2024

Preprint

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In flowering plants, the growth of pollen tubes carrying sperm cells to the female gametophyte is necessary for double fertilization and seed development. The rate of growth of the pollen tube tip determines the fertility of many artificially cultivated plants and depends on the vesicle-mediated transport of cell membrane and cell wall components. However, the investigation of vesicle transport is hampered by the lack of transgenic methods for economically important plants. Here, we developed a method to transiently inhibit vesicle activity using brefeldin A (BFA) and antisense oligodeoxynucleotides (AS-ODNs) targeting key genes in the wishbone flower (Torenia fournieri), which has been used in studies of sexual reproduction in plants. BFA disrupted vesicle gradient homeostasis, thereby altering cell wall deposition and pollen tube morphology. The AS-ODN targeting Torenia fournieri ANXUR (TfANX), which is implicated in maintaining pollen tube growth and is used as a marker to assess inhibition by AS-ODNs, penetrated cell membranes and inhibited the expression of target genes. The treatment of pollen tubes with AS-ODN against TfRABA4D, which is specifically expressed in pollen and involved in regulating vesicle targeting, resulted in pollen tubes with a bulging phenotype and disrupted pectin deposition, effects similar to those of BFA. In summary, vesicle-mediated mechanisms regulate the patterning of the pollen tube cell wall in T. fournieri and our findings could facilitate the genetic manipulation of horticultural crops.

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“…VTA1 is thought to be a cofactor of VPS4A/B, which catalyzes and disassembles membraneassociated ESCRT-III assemblies. VTA1 is also involved in the sorting and down-regulation of EGFR (by similarity) [9]. However, to date, the expression of VTA1 in LUAD and its prognostic value remain unclear.…”

Section: Introductionmentioning

confidence: 99%

High expression of VTA1 is an adverse prognostic factor in lung adenocarcinoma

Xingang Sun,

Ruixin Wu,

Xinjun Guan

et al. 2024

Cell Mol Biol (Noisy-le-grand)

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Lung adenocarcinoma (LUAD) is a common pathological type of non-small cell lung cancer; identifying preferable biomarkers has become one of the current challenges. Given that VTA1 has been reported associated with tumor progression in various human solid cancers but rarely reported in LUAD, herein, RNA sequencing data from TCGA and GTEx were obtained for analysis of VTA1 expression and differentially expressed gene (DEG). Furthermore, functional enrichment analysis of VTA1-related DEGs was performed by GO/ KEGG, GSEA, immune cell infiltration analysis, and protein-protein interaction (PPI) network. In addition, the clinical significance of VTA1 in LUAD was figured out by Kaplan-Meier Cox regression and prognostic nomogram model. R package was used to analyze incorporated studies. As a result, VTA1 was highly expressed in various malignancies, including LUAD, compared with normal samples. Moreover, high expression of VTA1 was associated with poor prognosis in 533 LUAD samples, as well as T stage T2&T3&T4, N stage N1&N2&N3, M stage M1, pathologic stage II&III&IV, and residual tumor R1&R2, et al. (P < 0.05). High VTA1 was an independent prognostic factor in Cox regression analysis; Age and cytogenetics risk were included in the nomogram prognostic model. Furthermore, a total of 4232 DEGs were identified between the high-and the low-expression group, of which 736 genes were up-regulated and 3496 genes were down-regulated. Collectively, high expression of VTA1 is a potential biomarker for adverse outcomes in LUAD. The DEGs and pathways recognized in the study provide a preliminary grasp of the underlying molecular mechanisms of LUAD carcinogenesis and progression.

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Vesicle trafficking in Arabidopsis pollen tubes

Cited by 13 publications

References 138 publications

Canonical Rab5 GTPases are essential for pollen tube growth through style in Arabidopsis

Canonical Rab5 GTPases are essential for pollen tube growth through style in Arabidopsis

Vesicle-Mediated Regulation of Pollen tube Growth inTorenia fournieri

High expression of VTA1 is an adverse prognostic factor in lung adenocarcinoma

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