Vesicle Viewer: Online visualization and analysis of small-angle scattering from lipid vesicles

Lewis-Laurent, Aislyn; Doktorova, Milka; Heberle, Frederick A.; Marquardt, Drew

doi:10.1016/j.bpj.2021.09.018

Cited by 9 publications

(8 citation statements)

References 45 publications

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“…To obtain real space information from these techniques where usable information like the bilayer thickness and area per lipid are present, a model based scattering density profile (SDP) was used to parse the membrane bilayer into three distinct segments. 35,64,65 The volume probabilities are shown in Figure 3A.…”

Section: ■ Resultsmentioning

confidence: 99%

“…34 SANS data were analyzed using Vesicle Viewer software. 35 Small angle X-ray scattering (SAXS) experiments were carried out using the 12-ID-B beamline at the Advanced Photon Source (Argonne National Laboratory, Lemont, IL). An average photon energy of 13.3 keV was used, and data were collected using a 2 M Pilatus detector (Dectris Ltd., Philadelphia, PA) set at a sample−distance of 2.0106 m. Xrays with a wavelength of 0.9322 Å were used.…”

Section: ■ Methodsmentioning

confidence: 99%

“…The resulting form factors in the scattering vector range of 0.03 Å −1 < Q < 0.9 Å −1 were background corrected using the established onsite reduction workflow and analyzed using Vesicle Viewer software. 35 All measurements were collected at 37 °C. LUVs at a concentration of 15 mg/mL in H 2 O were loaded into temperature controlled capillary-flow cells.…”

Section: ■ Methodsmentioning

confidence: 99%

“…The scattered beam was counted on a 2D- 3 He detector and subsequently corrected for detector pixel sensitivity, dark current, sample transmission, and background scattering from D 2 O, radially averaged, and stitched using Igor Pro and the NCNR developed reduction scripts . SANS data were analyzed using Vesicle Viewer software . Small angle X-ray scattering (SAXS) experiments were carried out using the 12-ID-B beamline at the Advanced Photon Source (Argonne National Laboratory, Lemont, IL).…”

Section: Methodsmentioning

confidence: 99%

“…An average photon energy of 13.3 keV was used, and data were collected using a 2 M Pilatus detector (Dectris Ltd., Philadelphia, PA) set at a sample–distance of 2.0106 m. X-rays with a wavelength of 0.9322 Å were used. The resulting form factors in the scattering vector range of 0.03 Å –1 < Q < 0.9 Å –1 were background corrected using the established on-site reduction workflow and analyzed using Vesicle Viewer software . All measurements were collected at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

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Probing the Link between Pancratistatin and Mitochondrial Apoptosis through Changes in the Membrane Dynamics on the Nanoscale

et al. 2022

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Pancratistatin (PST) is a natural antiviral alkaloid that has demonstrated specificity toward cancerous cells and explicitly targets the mitochondria. PST initiates apoptosis while leaving healthy, noncancerous cells unscathed. However, the manner by which PST induces apoptosis remains elusive and impedes the advancement of PST as a natural anticancer therapeutic agent. Herein, we use neutron spin−echo (NSE) spectroscopy, molecular dynamics (MD) simulations, and supporting small angle scattering techniques to study PST's effect on membrane dynamics using biologically representative model membranes. Our data suggests that PST stiffens the inner mitochondrial membrane (IMM) by being preferentially associated with cardiolipin, which would lead to the relocation and release of cytochrome c. Second, PST has an ordering effect on the lipids and disrupts their distribution within the IMM, which would interfere with the maintenance and functionality of the active forms of proteins in the electron transport chain. These previously unreported findings implicate PST's effect on mitochondrial apoptosis.

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Section: ■ Resultsmentioning

confidence: 99%

Section: ■ Methodsmentioning

confidence: 99%

Section: ■ Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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