Vesicles from Pluronic/poly(lactic acid) block copolymers as new carriers for oral insulin delivery

Xiong, Xiang‐Yuan; Li, Yu Ping; Li, Zi Ling; Zhou, Chenglong; Tam, Kam Chiu; Liu, Zhi Yong; Xie, Guo Xiu

doi:10.1016/j.jconrel.2007.04.004

Cited by 119 publications

(63 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It could be suggested that TMC/HPMCP nanoparticles had protective ability for entrapped HBsAg against degradation by gastric simulated condition. These results were consistent with that reported for papain, 21 insulin, 8,23 cyclosporine A, calcitonin, and somatostatin. 24 In this study, a novel TMC/HPMCP nanoparticle system was successfully developed for oral administration of HBsAg.…”

Section: Discussionsupporting

confidence: 92%

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Farhadian

Dounighi

Avadi³

2015

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

Oral vaccination is the preferred route of immunization. However, the degradative condition of the gastrointestinal tract and the higher molecular size of peptides pose major challenges in developing an effective oral vaccination system. One of the most excellent methods used in the development of oral vaccine delivery system relies on the entrapment of the antigen in polymeric nanoparticles. In this work, trimethyl chitosan (TMC) nanoparticles were fabricated using ionic gelation teqnique by interaction hydroxypropyl methylcellulose phthalate (HPMCP), a pHsensitive polymer, with TMC and the utility of the particles in the oral delivery of hepatitis B surface antigen (HBsAg) was evaluated employing solutions that simulated gastric and intestinal conditions. The particle size, morphology, zeta potential, loading capacity, loading efficiency, in vitro release behavior, structure, and morphology of nanoparticles were evaluated, and the activity of the loaded antigen was assessed. Size of the optimized TMC/HPMCP nanoparticles and that of the antigen-loaded nanoparticles were 85 nm and 158 nm, respectively. Optimum loading capacity (76.75%) and loading efficiency (86.29%) were achieved at 300 mg/mL concentration of the antigen. SEM images revealed a spherical shape as well as a smooth and near-homogenous surface of nanoparticles. Results of the in vitro release studies showed that formulation with HPMCP improved the acid stability of the TMC nanoparticles as well as their capability to preserve the loaded HBsAg from gastric destruction. The antigen showed good activity both before and after loading. The results suggest that TMC/HPMCP nanoparticles could be used in the oral delivery of HBsAg vaccine.

show abstract

Section: Discussionsupporting

confidence: 92%

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Farhadian

Dounighi

Avadi³

2015

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

show abstract

“…This amphiphilic block copolymer aggregates in an aqueous solution to form vesicular nanoparticles. The oral administration of insulin-loaded vesicles to diabetic mice resulted in the reduction of blood glucose levels -25% of the initial glucose level -which was maintained at this level for an additional 18.5 h [21].…”

Section: Polymeric Nanovesiclesmentioning

confidence: 94%

“…Conventionally, liposomal vesicles were developed by the self-assembly of phospholipid molecules in an aqueous environment. Recently, polymeric vesicles were prepared from amphiphilic polymers which form aggregates in aqueous solutions [21]. Solid lipid nanoparticles (SLN) are submicron colloidal carriers prepared from solid lipids (lipids being solid at room and body temperatures), such as triacylglycerols, complex acylglycerol mixtures or waxes, and dispersed either in water or in an aqueous surfactant solution [22].…”

Section: Typesmentioning

confidence: 99%

See 1 more Smart Citation

Oral Delivery of Insulin: Novel Approaches

Elsayed¹

2012

Recent Advances in Novel Drug Carrier Systems

View full text Add to dashboard Cite

“…The NPs delivery system is composed of poly (lactic acid) (PLA) and Pluronic 85 block copolymers (PEO-PPO-PEO) prepared by a simple and mild ionic-gelation method. More specifically, we have observed that PLA-P85-PLA-NPs are able to enhance and prolong the systemic absorption of the model drugs, administered orally [4]. However, the drug absorption in humans and absorption characteristics did not cleared.…”

Section: Introductionmentioning

confidence: 95%

Study on uptake of PLA-Pluronic P85-PLA nanoparticles with Caco-2 cells

Shu¹,

Huang²,

Yao³

et al. 2015

Proceedings of the 2015 International Conference on Advanced Engineering Materials and Technology

Self Cite

View full text Add to dashboard Cite

Abstract. We have shown that insulin-loaded Poly(lactic acid)-b-Pluronic-b-poly(lactic acid) nanoparticles (PLA-P85-PLA-NPs) had the hypoglycemic effect after oral administration on diabetic mice. In the present work, free insulin (INS) as controls, the influence of some factors such as time, temperature, pH level, and INS concentration on the uptake efficacy of PLA-P85-PLA-NPs as drug carriers were investigated through a Caco-2 cells. The micrographs of Caco-2 cell model were observed by microscopy. The cytotoxicity of PLA-P85-PLA-NPs in vitro was evaluated by MTT assay. The insulin concentrations in Caco-2 cells were determined by high-performance liquid chromatography (HPLC). Compared with INS groups, PLA-P85-PLA-NPs uptake was not controlled by pH levels. PLA-P85-PLA-NPs can be uptake by Caco-2 cells, and the absorption of PLA-P85-PLA-NPs is dependent on the concentration and incubation time. INS-loaded PLA-P85-PLA-NPs significantly enhanced insulin absorption. These results suggest that PLA-P85-PLA-NPs were more efficient than individual drugs into Caco-2 cells in a short period of time, and PLA-P85-PLA-NPs might be a useful drug carrier for proteins and peptides.

show abstract

Vesicles from Pluronic/poly(lactic acid) block copolymers as new carriers for oral insulin delivery

Cited by 119 publications

References 41 publications

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Enteric trimethyl chitosan nanoparticles containing hepatitis B surface antigen for oral delivery

Oral Delivery of Insulin: Novel Approaches

Study on uptake of PLA-Pluronic P85-PLA nanoparticles with Caco-2 cells

Contact Info

Product

Resources

About