Vesicular roundness and compound release in PC-12 cells

Germain, David; Maysinger, Dušica; Mi, Glavinovíc

doi:10.1016/j.jneumeth.2005.10.003

Cited by 6 publications

(6 citation statements)

References 79 publications

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“…The second expression is given by the ratio of the largest inner to the smallest outer concentric circumferences enclosing the vesicle surface (Figure D). Finally, the third expression is the ratio of the smallest to the largest inertia moment among the calculated principal moments of inertia of the vesicle (Figure E) …”

Section: Calculation Of Curvature-dependent Properties For Closed Che...mentioning

confidence: 99%

“…Finally, the third expression is the ratio of the smallest to the largest inertia moment among the calculated principal moments of inertia of the vesicle (Figure 7E). 17 Crystalline porous materials have a profusion of industrial applications in gas separation/storage, catalysis, and drug delivery. 18−23 More recently, this class of highly versatile materials have shown to conduct charge, opening the way for its use as charge storage devices, electrochemical sensors, and electrocatalysts.…”

Section: Properties For Closed Chemical Surfacesmentioning

confidence: 99%

“…Finally, the third expression is the ratio of the smallest to the largest inertia moment among the calculated principal moments of inertia of the vesicle ( Figure 7 E). 17 …”

Section: Calculation Of Curvature-dependent Properties For Closed Che...mentioning

confidence: 99%

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Surface Assessment via Grid Evaluation (SuAVE) for Every Surface Curvature and Cavity Shape

Santos

Coutinho

Soares

2022

J. Chem. Inf. Model.

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The surface assessment via grid evaluation (SuAVE) software was developed to account for the effect of curvature in the calculations of structural properties of chemical interfaces regardless of the chemical composition, asymmetry, and level of atom coarseness. It employs differential geometry techniques, enabling the representation of chemical surfaces as fully differentiable. In this article, we present novel developments of SuAVE to treat closed surfaces and complex cavity shapes. These developments expand the repertoire of curvature-dependent analyses already available in the previous version of SuAVE (e.g., area per lipid, density profiles, membrane thickness, deuterium-order parameters, volume per lipid, and surface curvature angle) to include new functionalities applicable to soft matter (e.g., sphericity, average radius, principal moment of inertia, and roundness) and crystalline porous materials (e.g., pore diameter, internal void volume, total area, and the total void volume of the unit cell structure). SuAVE can accurately handle chemical systems with high and low atom density as demonstrated for two distinct chemical systems: the lipid A vesicle and a set of selected metal–organic frameworks. The SuAVE software v2.0 is fully parallel and benefits from a compiler that supports OpenMP. SuAVE is freely available from and .

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Section: Calculation Of Curvature-dependent Properties For Closed Che...mentioning

confidence: 99%

Section: Properties For Closed Chemical Surfacesmentioning

confidence: 99%

See 1 more Smart Citation

Surface Assessment via Grid Evaluation (SuAVE) for Every Surface Curvature and Cavity Shape

Santos

Coutinho

Soares

2022

J. Chem. Inf. Model.

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“…However, if the second vesicle fuses just after the primary vesicle fuses, it could appear as a heel or a reverse shoulder depending on the timing of two (or more) vesicles fusing. One study, however, performed with PC12 cells showed that compound release was unlikely to occur as determined by vesicle size measurements and morphology of vesicles [30]. Future experiments can be performed to distinguish between these and additional possibilities that could explain the amperometric shapes of release events.…”

Section: Kineticsmentioning

confidence: 99%

Analysis of Amperometric Spike Shapes to Release Vesicles

et al. 2011

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Carbon-fiber amperometry is a real-time analytical tool to detect vesicle release events from individual secretory cells. This study compared the spike events released in response to local or global depolarization. We describe each spike shape that regularly occurs, as well as those shapes of spikes that occur with less frequency, but reproducibly. We compare how frequently each type of spike occurs, and compare their amplitudes and kinetics to the commonly accepted spike release events. Analysis of the amperometric peaks typically discarded from single cell amperometry recordings may reveal unappreciated phenomena related to the release of secretory vesicles.

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“…The value of Roundness increases with increase of circularity and becomes 1 for a perfect circle [18]. In present work, Roundness is adopted and the value of 0.7 is defined to distinguish the morphology between rod-shaped or flake-shaped and spherical particles.…”

Section: Determination Of Particle Size Distributionmentioning

confidence: 99%