Vesicular stomatitis virus infects and matures only through the basolateral surface of the polarized epithelial cell line, MDCK

Do lipid rafts mediate virus assembly and pseudotyping?

“…Rodriguez-Boulan et al (1983) showed that influenza viruses, such as WSN, bud from the apical surface of polarized Madin-Darby canine kidney cells, while VSV buds from the basolateral surface. This has been demonstrated amply in singly infected cells (Fuller et al, 1984;Fuller, 1987;Pfeiffer et al, 1985;Roth et al, 1979;. During early infection in doubly infected cells, both viruses maintain their polar budding (Roth & Compans, 1981).…”

Jaagsiekte sheep retrovirus pseudotyped lentiviral vector-mediated gene transfer to fetal ovine lung

“…In sheep, VSVg-LV injected into the fetal airways between days 75 and 77 achieved limited airway epithelial transduction. 32 The receptor for VSVg is thought to be located on the basolateral surface of the respiratory epithelium, 34,35 which may explain, in part, inefficient gene transfer to the fetal respiratory epithelium in sheep. 32 Designing pseudotyped LV to target airway epithelial surface receptors is an effective strategy to enhance lung gene transfer.…”

Sorting and endocytosis of viral glycoproteins in transfected polarized epithelial cells.

“…Such detergent treatment does not interfere with the visualization of antigen molecules located in the apical surface. In fluorescent micrographs of permeabilized monolayers, antigens present in the basolateral surface give rise to a polygonal pattern that is generated by the outlines of adjacent cells (9,21,23,25,28,29,32,33), Using immunoelectron microscopy, previous studies (28,29) have shown that the appearance of such a contour fluorescence pattern correlates with the arrival of the basolaterally located protein at the cell surface. Optimal visualization of apically and basolaterally located proteins is achieved when the plane of focus is placed at the surface of the monolayer or within the cell, respectively.…”