Abstract--The ultrasonic velocity at 100MHz and room temperature (23-26°C) was measured in fresh, excised mammalian liver, kidney, spleen, and pancreas and mouse kidney. The results show excellent agreement with measurements at low MHz frequencies. A significant velocity difference is reported between kidney cortex and medulla, but no difference was observed between the exocrine and islets of Langerhans in the first measurements of velocity in the pancreas.