VIABILIDADE DE SEMENTES DE Senegalia polyphylla PELO TESTE DO TETRAZÓLIO

Lima, Tamires Leal de; Cunha, Marina Silva da

doi:10.28998/rca.v17i3.7406

Cited by 1 publication

(2 citation statements)

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“…The 0.5% tetrazolium concentration, regardless of immersion time, created an intense staining (Table 2), making it more difficult to differentiate between viable and non-viable embryos. This was observed by T. L. Lima and Cunha (2019) in S. polyphylla, at the 0.5% concentration, for which all soaking and immersion times resulted in strong staining, which made it difficult to interpret and differentiate normal and firm tissues from those in deterioration processes. Our findings agree with those described by Pereira et al (2020), who investigated P. stipulacea seeds and stated that staining patterns vary with the solution concentration (0.05, 0.075, and 0.1%) and incubation time (2, 4, and 6 h).…”

Section: Resultsmentioning

confidence: 99%

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Suitability of tetrazolium test for Tamarindus indica L. seeds

Cordeiro

Bruno

Lima

et al. 2022

Semina: Ciênc. Agrár.

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Seed quality is routinely assessed by direct tests, e.g, the germination test, or indirect tests like the tetrazolium test, which has shoown to be promising in the determine viability and vigor, allowing the diagnosis of the main problems that may affect seed quality, such as mechanic damages, field deterioration and storage. In this respect, this study was conducted to develop a tetrazolium test protocol to evaluate the viability and vigor of Tamarindus indica L. seeds. Before exposing the seeds to the tetrazolium solution, seed preconditioning studies were carried out in which seven soaking times were tested. The soaking time that did not cause damage to the seed embryo and allowed the removal of the seed coat to expose the seed structures to the tetrazolium salt was selected. Then, an experiment was set up in a completely randomized design with a 2x3x3 factorial arrangement involving two seed lots, three soaking times in tetrazolium salt (6, 12 and 16 h) and three salt concentrations (0.075, 0.1 and 0.5%), totaling 18 treatments with four replicates of 25 seeds, evaluated at 40 ºC. For each treatment, the seeds were divided into three classes, namely, viable and vigorous embryos (class 1); viable embryos (class 2) and non-viable or dead embryos (class 3). For a comparison with the tetrazolium test results, the two seed lots were analyzed for water content, germination, emergence, first count, germination speed index, emergence speed index, growth and seedling dry weight. The viability and vigor of T. indica seeds can be evaluated after a soaking period of 48 h and immersion for 6h in tetrazolium salt at the concentration of 0.1%, at 40°C, with provides results similar to conventional seed viability tests. The tetrazolium test proved to be adequate to differentiate T. indica seed lots in terms of viability.

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Section: Resultsmentioning

confidence: 99%

“…L. P. Queiroz (Sousa, Bruno, Silva, Torres, & Andrade, 2017), Senegalia polyphylla (DC.) Britton and Rose (T. L. Lima & Cunha, 2019), and Piptadenia stipulacea (Benth.) Ducke (Pereira, Paiva, Souza, Benedito, & Torres, 2020).…”

Section: Indicamentioning

confidence: 99%