Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem

Luciano, Barbosa Munita; Kanazawa, Mábilis Yumi; Peres, Anelise Ribeiro; Souza, Fabiana Ferreira de

doi:10.4322/rbcv.2014.097

Cited by 2 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The glycerol concentrations for semen cryopreservation have been studied at several years. However, there are few studies on cryopreservation of semen from bulls epididymal source, which report successful cryopreservation using a wide range of glycerol concentrations, from 4% to 8% (Barbosa, Kanazawa, Peres, & Souza, ; Martins et al., , ).…”

Section: Discussionmentioning

confidence: 99%

Effects of glycerol, equilibration time and antioxidants on post-thaw functional integrity of bovine spermatozoa directly obtained from epididymis

et al. 2016

View full text Add to dashboard Cite

This work aimed to evaluate the effect of stabilisation times, glycerol concentration, and the catalase and superoxide dismutase supplementation of diluent on parameters of frozen-thawed spermatozoa from epididymis of Nelore bulls: Experiment 1: spermatozoa diluted in Tris-egg yolk with glycerol (3%, 5% or 7%) and stabilisation times (0, 2 or 4 hr at 5°C); Experiment 2: Tris-egg yolk only, Tris-egg yolk with catalase (CAT, 50 or 100 U ml ) or superoxide dismutase (SOD, 50 or 100 U ml ). Frozen-thawed spermatozoa were evaluated for kinetic parameters, plasma membrane and acrosome integrity, mitochondrial activity and IVF capacity. ALH and BCF were affected (p < .05) by glycerol at 3% after 4-hr equilibration time and 7% after 2-hr equilibration time. Glycerol 3% had lower (p < .05) iPM and iAc after 4 hr. Glycerol 5% had greater (p < .05) hPMM after 4 hr and iAc after 2 hr than at 0 hr. SOD 100 U ml had lower (p < .05) linearity and wobble compared to control group. No was observed differences to fertilisation rate (p < .05) among groups. In conclusion, glycerol 5% in Tris-egg yolk extender for 4 hr is suitable for the preservation of sperm kinetics and membrane integrity. CAT (50 and 100 U ml ) or SOD (50-100 U ml ) had no beneficial effects on sperm kinetics, plasma and acrosomal membrane integrity, mitochondrial activity or the capacity for IVF of frozen-thawed spermatozoa from epididymis of Nelore bulls.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of glycerol, equilibration time and antioxidants on post-thaw functional integrity of bovine spermatozoa directly obtained from epididymis

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Barbosa et al (2012) compararam a qualidade das células espermáticas do epidídimo, pré e pós-congelação e verificaram motilidade em torno de 30% nas células descongeladas. Tal achado indica que estas amostras podem ser utilizadas na inseminação artificial, já que na maioria a qualidade obtida se iguala aos parâmetros obtidos em centrais de comercialização do sêmen (Crespilho et al, 2009).…”

Section: Introductionunclassified

Viabilidade dos espermatozoides criopreservados de touros colhidos do epidídimo após 12 horas a 4ºC

Scott

Lourenço

Moura

et al. 2016

RBCV

View full text Add to dashboard Cite

ResumoO objetivo deste estudo foi avaliar o efeito da refrigeração do epidídimo, sobre a viabilidade dos espermatozoides congelados. Foram colhidos dez pares de testículos/epidídimos de um abatedouro comercial. Um dos epidídimos foi refrigerado a 4°C durante 12 horas e o contralateral foi imediatamente processado. O epidídimo foi isolado, as células espermáticas extraídas e analisadas quanto à motilidade, vigor, concentração, morfologia e integridade da membrana. Após a análise, os espermatozoides foram congelados em diluente Botubov ® (Botupharma Biotecnologia Animal, Botucatu, SP, Brasil) e descongelados para análise. O mesmo procedimento foi realizado com o testículo/epidídimo refrigerado. Os resultados evidenciaram maior viabilidade (p≤0,05) das células pré-congelação para os parâmetros, número total de espermatozides (1,9 ± 1,2 versus 0,9 ± 0,9 x 10 9 espermatozoides), motilidade (74,0 ± 15,1 versus 20,5 ± 13,8%) e vigor (3,7 ± 0,5 versus 1,7 ± 0,8), e pós-congelação, motilidade (23,5 ± 16,7 versus 8,0 ± 7,9%) e vigor (2,0 ± 0,8 versus 0,8 ± 0,8) quando os espermatozoides foram colhidos a partir de epidídimos processados imediatamente após o abate quando comparados aos mantidos 12 horas sob refrigeração, respectivamente. Conclui-se que 12 horas de refrigeração do epidídimo após o abate, prejudica a qualidade das células espermáticas, impossibilitando a congelação do sêmen.Palavras-chave: bovino, cauda-epidídimo, célula-espermática, congelação. AbstractThe purpose of this study was to evaluate effect of epididymis cooling on bovine frozen sperm viability. Ten pairs of testes/epididymes were collected of a commercial slaughterhouse; one epididymis from each pair was refrigerated at 4ºC for 12 hours and the other immediately proceeded. Epididymis was isolated, sperm cells collected after epididymal slicing and then analyzed regarding to motility, vigor, total number of sperm, morphology and membrane integrity. Sperm cells were frozen in Botubov® extender (Botupharma Biotecnologia Animal, Botucatu, SP, Brazil) and thawed for semen analysis. The same procedure was performed with cooled testis/epididymis. Results demonstrated higher viability (p≤0,05) of fresh cells to the total number of spermatozoa (1,9 ± 1,2 versus 0,9 ± 0,9 x 10 9 spermatozoa), sperm motility (74,0 ± 15,1 versus 20,5 ± 13,8%) and vigor (3,7 ± 0,5 versus 1,7 ± 0,8), and for pos-thawing motility (23,5 ± 16,7 versus 8,0 ± 7,9%) and vigor (2,0 ± 0,8 versus 0,8 ± 0,8) when spermatozoa were collected immediately post-slaughter than maintained cooling 12 hours, respectively. We conclude that 12 hours of epididymis cooling after slaughter decreases sperm cells quality.

show abstract

Viabilidade do sêmen congelado obtido do epidídimo de touros post-mortem

Cited by 2 publications

References 4 publications

Effects of glycerol, equilibration time and antioxidants on post-thaw functional integrity of bovine spermatozoa directly obtained from epididymis

Effects of glycerol, equilibration time and antioxidants on post-thaw functional integrity of bovine spermatozoa directly obtained from epididymis

Viabilidade dos espermatozoides criopreservados de touros colhidos do epidídimo após 12 horas a 4ºC

Contact Info

Product

Resources

About