Viability and pluripotency studying of human embryo stem cells labeled with quantum dots

Ruan, Jing; Shen, Jie; Shi, Hua; Ji, ajia; Wang, Kan; Cui, Daxiang; Wang, Zheng

doi:10.5101/nbe.v2i4.p245-251

Cited by 8 publications

(7 citation statements)

References 29 publications

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“…Other approach to identification of tumor cells is the use of quantum dots, powerful optically contrast agents enabling the monitoring of the tumor in vivo [ 14 ]. The ability of the quantum dots to non-invasively visualize the human embryonic stem cells in vivo testifies into favor of their possible biomedical application [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Nanotechniques Inactivate Cancer Stem Cells

et al. 2017

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One of the tasks of current oncology is identification of cancer stem cells and search of therapeutic means capable of their specific inhibition. The paper presents the data on phenotype characteristics of Ehrlich carcinoma cells as convenient and easy-to-follow model of tumor growth. The evidence of cancer stem cells as a part of Ehrlich carcinoma and significance of CD44+ and CD44– subpopulations in maintaining the growth of this type of tumor were demonstrated. A high (tenfold) tumorigenic activity of the Ehrlich carcinoma CD44+ cells if compared to CD44– cells was proven. In this pair of comparison, the CD44+ cells had a higher potential of generating in peritoneal cavity of CD44high, CD44+CD24–, CD44+CD24+ cell subpopulations, highlighting the presence of cancer stem cells in a pool of CD44+ cells.In this study, the ability of synthesized hybrid nanocomplexes, comprising the nanoparticles of rare earth orthovanadates GdYVO4:Eu3+ and cholesterol to inhibit the tumor growth and to increase the survival of the animals with tumors was established. A special contribution into tumor-inhibiting effect is made by each of its components. Treatment of Ehrlich carcinoma cells with two-component hybrid complex resulted in maximum reduction in the concentration of the most tumorigenic CD44high cells with simultaneous rise in the number of CD117+ cells that decreased an intensity of tumor growth by 74.70 ± 4.38% if compared with the control.

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Section: Introductionmentioning

confidence: 99%

Nanotechniques Inactivate Cancer Stem Cells

et al. 2017

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“…In addition, the broad emission spectrum of the donor, with its long red tail, can often overlap significantly with the emission spectrum of the acceptor. Several recent reports have confirmed that luminescent semiconductor quantum dots (QDs), such as CdSe and CdTe, are able to participate in resonance energy transfer processes analogous to FRET [7][8][9][10][11][12], which makes these materials good candidates to overcome some of the limitations associated with conventional organic dye molecules in FRET-based studies of biomolecular structure, ligandreceptor binding, etc.…”

Section: Introductionmentioning

confidence: 97%

Fluoroimmunoassay for Antigen Based on Fluorescence Resonance Energy Transfer Between Quantum Dots and Gold Nanoparticles

Wang¹,

You²,

Fu³

et al. 2013

Nano BioMed ENG

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A unique, sensitive, and highly specific fluoroimmunoassay system for antigen detection using quantum dot and gold nanoparticles has been developed. The assay is based on the fluorescence quenching of quantum dots caused by gold nanoparticles coated with antibody. To demonstrate its analytical capabilities, the quantum dots were coated with anti-HBsAg monoclonal antibodies (QDs-MAb1) and gold nanoparticles coated with another anti-HBsAg monoclonal antibodies ( GNPs-MAb2) which specifically bound with HBsAg could sandwich the HBsAg captured by the immunoreactions. The sandwich-type immunocomplex was formed and the energy of quantum dots was transferred to gold nanoparticles as they were within a short distance, so that the fluorescence intensity of quantum dots was quenched. The fluorescence intensity of quantum dots at 570 nm was negative linear proportional to HBsAg concentration logarithm. The result showed that the limit of detection of the HBsAg was 0.928 ng/mL. This new system can be extended to detect target molecules with matched antibodies and has broad potential applications in immunoassay and disease diagnosis.

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“…Quantum dots (QDs) have been subjected to intensive investigations because of their unique photoluminescence properties and potential applications. In the past, QDs have been used successfully in cellular imaging . In particular, Cd‐based quantum, such as CdSe and CdSe/ZnS nanoparticles (NPs), fluorophores are of immense interest for their potential applications in optical devices and as probes for biological imaging and labeling.…”

Section: Introductionmentioning

confidence: 99%

Subchronic intravenous toxicity study of biofunctional ZnO and its application as a fluorescence probe for cell‐specific targeting

Nakamura

Watanabe

2018

J Biochem & Molecular Tox

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Successful development of safe and highly effective nanoprobes for targeted imaging of in vivo early cancer is a great challenge. Herein, we choose the visible‐light emitting zinc oxide non–core/shell type nanoparticle (NP) fluorophores (ZHIE) as prototypical materials. We have reported on these materials previously. The results showed that the ZHIE NPs exhibited good water solubility and good biocompatibility. This study was conducted to investigate the toxicity of ZHIE NPs when intravenously administered to mice repeatedly at the dose required for successful tumor imaging in vivo. Anti‐macrophage‐1 antigen (Mac1), a macrophage differentiation antigen, antibody‐conjugated ZHIE NPs successfully realized targeted imaging of murine macrophage cell line Raw264.7 cells. In conclusion, ZHIE NPs are not toxic in vivo and antibody‐conjugated ZHIE NPs have great potential in applications, such as single cell labeling.

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Viability and pluripotency studying of human embryo stem cells labeled with quantum dots

Abstract: Semiconductor quantum dots (QDs) hold increasing potential for cellular imaging both in vitro and in vivo. In this report, we aimed to study imaging

Cited by 8 publications

References 29 publications

Nanotechniques Inactivate Cancer Stem Cells

Nanotechniques Inactivate Cancer Stem Cells

Fluoroimmunoassay for Antigen Based on Fluorescence Resonance Energy Transfer Between Quantum Dots and Gold Nanoparticles

Subchronic intravenous toxicity study of biofunctional ZnO and its application as a fluorescence probe for cell‐specific targeting

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