Viability and removal assessment of Escherichia coli and Salmonella spp. by real-time PCR with propidium monoazide in the hygienization of sewage sludge using three anaerobic processes

Flores-Ramírez, Aarón; Ortega-Cuenca, J.; Cuetero-Martínez, Yovany; Cobos, D. de los; Noyola, A.

doi:10.1016/j.wasman.2023.02.019

Cited by 2 publications

(1 citation statement)

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“…DNA extraction is performed on the pretreated sample, and the dye-bound DNA from non-viable cells is purposefully excluded during this extraction. The DNA extracted from viable cells is then subjected to molecular detection methods, offering a highly accurate means of pathogenic detection [39].…”

Section: Dye Treatment Methodsmentioning

confidence: 99%

Advances in Pretreatment Methods for Free Nucleic Acid Removal in Wastewater Samples: Enhancing Accuracy in Pathogenic Detection and Future Directions

Vu,

Nguyen,

Nguyen

2023

Preprint

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Accurate pathogenic detection in wastewater is critical for safeguarding public health and the environment. However, the presence of free nucleic acids in wastewater samples poses significant challenges to molecular detection accuracy. This comprehensive review explores the current status and future potential of pretreatment methods to remove free nucleic acids from wastewater samples. The study contributes a comprehensive analysis of the mechanisms, strengths, and limitations of various pretreatment approaches, including physical, chemical, and enzymatic processes. The effect of various factors on the removal efficiency of these pretreatment methods is also discussed. This review enhances our comprehension of pretreatment techniques and their vital role in achieving precise pathogenic detection in complex wastewater matrices. Furthermore, it outlines future perspectives and developments for improving the speed and effectiveness of pathogenic detection, contributing significantly to disease surveillance, early warning systems, and environmental protection.

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Section: Dye Treatment Methodsmentioning

confidence: 99%