Viability and Stability of Yeast Cells and Filamentous Fungus Spores During Freeze-Drying: Effects of Protectants and Cooling Rates

Berny, J.-F.; Hennebert, G. L.

doi:10.1080/00275514.1991.12026086

Cited by 56 publications

(33 citation statements)

References 32 publications

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“…Rate of cooling, size of propagules, and thickness of cell wall are important parameters not considered in this study (Berny & Hennebert, 1991;Schoenlein-Crusius et al, 1993;Tan et al, 1995), and they may explain the observed intra and interspecific variability of fungal cultures as measured by conidial viability after different storage periods.…”

mentioning

confidence: 99%

“…Although attempts to improve conidial viability of lyophilized fungal cultures were not successful, such as the combinations of protectants [skim milk (10%), glutamic acid (5%) and raffinose (10%)] suggested by Berny & Hennebert (1991) and the use of another lyophilization apparatus (Supermodulyo 12K Freeze Dryer, Edwards, USA), all strains tested showed some degree of viability.…”

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confidence: 99%

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Entomopathogenic fungal (Hyphomycetes) collection: assessment of conidial viability

Faria

Martins

Mello

et al. 1999

Pesq. agropec. bras.

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-Twenty four strains of the entomopathogenic fungi (Hyphomycetes) Beauveria bassiana, Metarrhizium anisopliae, Nomuraea rileyi, Paecilomyces farinosus, P. fumosoroseus and P. lilacinus, maintained in the culture collection of Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia (Cenargen) and preserved by lyophilization and in liquid nitrogen, had their conidial viability assessed. Germination rates of 16-to 84-month-old cultures stored in liquid nitrogen decreased, on average, less than 13.3%. For 29-to 49-month-old cultures preserved by lyophilization, the viability loss ranged, on average, from 28.6 to 94.5%. The results demonstrated the efficiency of the tested methods, especially liquid nitrogen, in preserving the viability of entomopathogenic fungi. COLEÇÃO DE FUNGOS ENTOMOPATOGÊNICOS (HYPHOMYCETES):AVALIAÇÃO DA VIABILIDADE CONIDIAL RESUMO -Vinte e quatro isolados dos fungos entomopatogênicos (Hyphomycetes) Beauveria bassiana, Metarrhizium anisopliae, Nomuraea rileyi, Paecilomyces farinosus, P. fumosoroseus e P. lilacinus, mantidos na coleção de culturas da Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia (Cenargen) e preservados em nitrogênio líquido e liofilização, tiveram a viabilidade conidial avaliada. As taxas de germinação de conídios preservados em nitrogê-nio líquido diminuíram, em média, menos de 13,3% após 16 a 84 meses de armazenamento. A perda de viabilidade de isolados liofilizados, após 29 a 49 meses de armazenamento, variou de 28,6 a 94,5%. Os resultados demonstraram a eficiência dos métodos testados, especialmente nitrogênio líquido, na preservação da viabilidade de fungos entomopatogênicos.In Brazil, the occurrence of over 20 genera of entomopathogenic fungi has been reported (Alves, 1992). Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia (Cenargen), in Brasília, Brazil, is engaged in establishing an entomopathogenic fungal culture collection

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mentioning

confidence: 99%

mentioning

confidence: 99%

Entomopathogenic fungal (Hyphomycetes) collection: assessment of conidial viability

Faria

Martins

Mello

et al. 1999

Pesq. agropec. bras.

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“…After 3-4 yr, cultures of all five species of Dichotomocladium Benny & R.K.Benjamin and Syzygites megalocarpus Ehrenberg: Fries were no longer viable. The method proposed by Berny and Hennebert (1991) might extend the viability of these and other taxa.…”

Section: Maintenancementioning

confidence: 99%

“…6), or stored under nitrogen or even frozen at 2185uC if the facilities are available. Berny and Hennebert (1991) recommended lyophilizing cultures using a 3uC/min cooling rate in 10% skim milk containing 5% sodium glutamate plus one of the following: 5 or 10% honey, 10% raffinose, or 10% trehalose. Other cryoprotectants include dimethylsulfoxide (DMSO) or 10% glycerol (Smith and Onions 1983).…”

Section: Maintenancementioning

confidence: 99%

Methods Used by Dr. R. K. Benjamin, and Other Mycologists, to Isolate Zygomycetes

Benny¹

2008

aliso

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The methods that Dr. Richard K. Benjamin used to isolate Zygomycetes are discussed. These processes involved the following five steps: (1) collection, (2) plating, (3) isolation, (4) culture, and (5) maintenance. Additional methods, materials and modifications used to isolate Zygomycetes are summarized. The author considers the flattening of the aerial hyphae onto the substrate of the faster-and higher-growing Mucorales for several consecutive days to be the critical step in isolating species of Coemansia, Piptocephalis, Syncephalis, and Dimargaritales. The methods used by other scholars to isolate, culture, and study many taxa in Zygomycetes also are discussed.

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“…In which, freeze-drying can not only keep yoghurt fresh but maintains a sufficient quantity of viable probiotics (Capela et al, 2006), is proved to be a common way to preserve microorganisms for a long time (Berny and Hennebert, 1991). Freeze-drying is preferred to be a suitable drying technique for the conservation of micro-organisms (Morgan et al, 2006, Stephan et al, 2016.…”

Section: Introductionmentioning

confidence: 99%

Optimization of cryoprotectants for Streptococcus thermophilus during freeze-drying using Box-Behnken experimental design

Zhang

Hui

Chen

et al. 2017

Emir. J. Food Agric

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Viability and Stability of Yeast Cells and Filamentous Fungus Spores During Freeze-Drying: Effects of Protectants and Cooling Rates

Cited by 56 publications

References 32 publications

Entomopathogenic fungal (Hyphomycetes) collection: assessment of conidial viability

Entomopathogenic fungal (Hyphomycetes) collection: assessment of conidial viability

Methods Used by Dr. R. K. Benjamin, and Other Mycologists, to Isolate Zygomycetes

Optimization of cryoprotectants for Streptococcus thermophilus during freeze-drying using Box-Behnken experimental design

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