Viability of Acanthamoeba after exposure to a multipurpose disinfecting contact lens solution and two hydrogen peroxide systems

Hiti, K.; Walochnik, Julia; Haller-Schober, Eva-Maria; Faschinger, Christoph; Aspöck, Horst

doi:10.1136/bjo.86.2.144

Cited by 87 publications

(57 citation statements)

References 23 publications

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“…However, studies comparing the T4 and T5 genotypes for resistance to multipurpose solutions, curiously, showed that the T5 genotype has a higher resistance (Hiti et al 2002;Shoff et al 2007). Only isolates of the T2, T3, T4, T6, T11 (Walo- (Spanakos et al 2006;Iovieno et al 2010) genotypes have been associated with keratitis.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

Aguiar

Silveira

Winck

et al. 2013

Acta Parasitologica

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The present study investigated the susceptibility of Acanthamoeba spp. trophozoites to two multipurpose systems for cleaning and maintenance of contact lenses. Three strains of trophozoites from the ATCC (A. castellani T4, A. castellani Neff, and A. polyphaga) and two Acanthamoeba isolates obtained from swimming pools (PT5 and PO1) were placed in monoxenic culture. To test their survival in cleaning solutions for contact lenses, the trophozoites were exposed for 4 and 24 h to two multipurpose solutions (A and B), and were then inoculated into a new monoxenic culture. Amoebic growth on the plates was observed after 72 h of incubation. Trophozoites from all three ATCC strains and one isolate from a swimming pool (PO1) grew in all plates after 4 h of exposure to solutions A and B. After 24 h, the ATCC strains and the PO1 isolate showed growth in most of the plates treated. Only the PT5 isolate showed susceptibility to both solutions over the time intervals tested. The two solutions were not completely effective against most strains and isolates over the time intervals tested. These results are important, since species of Acanthamoeba are widely distributed in the environment and are potential agents of eye pathologies.

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Section: Discussionmentioning

confidence: 99%

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

Aguiar

Silveira

Winck

et al. 2013

Acta Parasitologica

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show abstract

“…Further complicating the issue are experimental animal and tissue culture models that have shown T5 isolates to be capable of a high degree of pathogenicity (33,34). Additionally, studies comparing T4 and T5 resistance to multipurpose contact lens cleaning solutions, interestingly, show that the T5 genotype possesses a better resistance (16,27). It is possible that the majority of T5 Acanthamoeba isolates may not be pathogenic to humans, but as the number of people that wear contact lenses continues to grow, the risk of encountering pathogenic T5 isolates may increase.…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of T4 and T5 Genotypes in Isolates from Acanthamoeba Keratitis Patients

et al. 2009

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Acanthamoeba keratitis (AK) is a rare but sight-threatening ocular infection. Outbreaks have been associated with contaminated water and contact lens wear. The epidemiology and pathology may be associated with unique genotypes. We determined the Rns genotype for 37 clinical isolates from 23 patients presenting at the University of Miami Bascom Palmer Eye Institute with confirmed AK infections in 2006 to 2008. The genus-specific ASA.S1 amplicon allowed for rapid genotyping of the nonaxenic cultures. Of the 37 isolates, 36 were of the T4 genotype. Within this group, 13 unique diagnostic fragment 3 sequences were identified, 3 of which were not in GenBank. The 37th isolate was a T5, the first in the United States and second worldwide to be found in AK. For five patients with isolates from the cornea and contact lens/case, identical sequences within each patient cluster were observed, confirming the link between contact lens contamination and AK infection. Genotyping is an important tool in the epidemiological study of AK. In this study, it allowed for the detection of new strains and provided an etiological link between source and infection. Additionally, it can allow for accurate categorizing of physiological differences, such as strain virulence, between isolates and clades.

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“…Several studies have shown that the sensitivity of Acanthamoeba cysts against contact lens disinfectants varies between the different strains and morphological groups. 19,23,[27][28] Acanthamoeba cysts are slightly smaller than the trophozoites with a size of 12-28 mm in diameter. They are double-walled consisting of an interior endocyst, which can be stellate, polygonal or even round, and an exterior wrinkled ectocyst.…”

Section: Discussionmentioning

confidence: 99%

One- and two-step hydrogen peroxide contact lens disinfection solutions against Acanthamoeba: How effective are they?

Hiti

Walochnik

Faschinger

et al. 2004

Eye

Self Cite

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Purpose Effective contact lens disinfection solutions are important to keep the storage case free of acanthamoebae and thus prevent an infection of the eye. The aim of our study was to evaluate the effectivity of two new onestep hydrogen peroxide disinfecting solutions against Acanthamoeba spp. and compare it to the effectivity of other commercially available systems. Methods Nine one-step 3% hydrogen peroxide systems including the new systems Silver Sept (platinum and silver disk for intensifying disinfection) and Blue Vision (newly composed catalytic tablet) and 2 two-step systems (0.6 and 3.0% H 2 O 2 ) were tested for their effectivity against cysts of two Acanthamoeba keratitis isolates at different concentrations.Results After a soaking time of 8 h (overnight soaking of contact lenses) the 2 two-step systems completely destroyed the cysts of both Acanthamoeba strains, even at the highest concentration of cysts tested. The nine tested one-step systems showed weaker effects. The new Blue Vision system was able to eradicate the cysts of one strain at the low concentration of cysts. Conclusions One-step hydrogen peroxide systems do not have sufficient effects on Acanthamoeba cysts and therefore may not protect the contact lens user from a possible infection of the eye. Further development of tablets like the ones used in the Blue Vision system may result in better cysticidal effects for one-step systems.

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Viability of Acanthamoeba after exposure to a multipurpose disinfecting contact lens solution and two hydrogen peroxide systems

Cited by 87 publications

References 23 publications

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

Molecular Identification of T4 and T5 Genotypes in Isolates from Acanthamoeba Keratitis Patients

One- and two-step hydrogen peroxide contact lens disinfection solutions against Acanthamoeba: How effective are they?

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