Viability of freeze dried microencapsulated human retinal pigment epithelial cells

Wikström, Jonna; Elomaa, Matti; Nevala, Laura; Räikkönen, Johanna; Heljo, Petteri; Urtti, Arto

doi:10.1016/j.ejps.2012.06.014

Cited by 21 publications

(8 citation statements)

References 35 publications

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“…36% using the optimal conditions is not within the acceptable range for reproducibility and scaleup from laboratory R&D to commercial scale processes. Moreover, the reported yields in this study are significantly lower than those reported across various mammalian cell lines in the literature (Eroglu et al 2000, He 2011, Lee et al 2013, Limaye and Kale 2001, Mart ın-Ib añez et al 2012, Serra et al 2011, Wikstr€ om et al 2012, Zhang et al 2010) specifically, 60% post-thawing efficiency has been reported for HEK cells using 5% DMSO dissolved in histidine-tryptophan-ketoglutarate medium (Chaytor et al 2012), which is at least 70% higher than the values obtained for the optimal conditions in 2D using DMSO (Run 8). The latter can be explained by higher viability efficiencies immediately after thawing relative to those observed after 1-7 days culture of the thawed cells (Heng et al 2006).…”

Section: Comparison With Other Cryoprotectantscontrasting

confidence: 72%

“…In the quiescent state, cells enter an amorphous/glassy phase characterized by low molecular mobility and activity to arrest biophysical and biochemical cellular activities. Successful preservation is governed by complex interactions between the type and concentration of cryo/lyoprotectant cocktails, kinetics of liquid water removal enhanced by 3D conformation, as well as cell-cell and cell-matrix interactions and specific properties of the individual IC cell lines (He 2011, Mart ın-Ib añez et al 2012, Serra et al 2011, Wikstr€ om et al 2012, Zhang et al 2010. The critical variables leading to cryo-injury, use of cryoprotective agents (CPAs) and biophysics of mammalian cell cryopreservation for optimal recovery rates based on modeling of cell dehydration and intracellular ice formation have been extensively reviewed by He (2011) and Mart ın-Ib añez et al (2012).…”

Section: Introductionmentioning

confidence: 99%

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Trehalose effectiveness as a cryoprotectant in 2D and 3D cell cultures of human embryonic kidney cells

Hara

Tottori

Anders

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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Post cryopreservation viability of human embryonic kidney (HEK) cells under two-dimensional (2D) and three-dimensional (3D) culture conditions was studied using trehalose as the sole cryoprotective agent. An L (3) Taguchi design was used to optimize the cryoprotection cocktail seeding process prior to slow-freezing with the specific aim of maximizing cell viability measured 7 days post thaw, using the combinatorial cell viability and in-vitro cytotoxicity WST assay. At low (200 mM) and medium (800 mM) levels of trehalose concentration, encapsulation in alginate offered a greater protection to cryopreservation. However, at the highest trehalose concentration (1200 mM) and in the absence of the pre-incubation step, there was no statistical difference at the 95% CI (p = 0.0212) between the viability of the HEK cells under 2D and 3D culture conditions estimated to be 17.9 ± 4.6% and 14.0 ± 3.6%, respectively. A parallel comparison between cryoprotective agents conducted at the optimal levels of the L study, using trehalose, dimethylsulfoxide and glycerol in alginate microcapsules yielded a viability of 36.0 ± 7.4% for trehalose, in average 75% higher than the results associated with the other two cell membrane-permeating compounds. In summary, the effectiveness of trehalose has been demonstrated by the fact that 3D cell cultures can readily be equilibrated with trehalose before cryopreservation, thus mitigating the cytotoxic effects of glycerol and dimethylsulfoxide.

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Section: Comparison With Other Cryoprotectantscontrasting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Trehalose effectiveness as a cryoprotectant in 2D and 3D cell cultures of human embryonic kidney cells

Hara

Tottori

Anders

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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“…To achieve higher concentration of intracellular trehalose, other loading methods such as freezing induced loading [59] or acetylation of trehalose [1] could be implemented. In addition, by extending the loading time for trehalose, the amount of intracellular trehalose increases [56]. However, HepG2 cell spheroids share less total surface area with surrounding media which might reduce the efficacy of given methods.…”

Section: Discussionmentioning

confidence: 99%

“…In anhydrobiotic organisms the amount of trehalose is typically high [9]. In addition, intracellular trehalose appears to protect DNA integrity, mononuclear cells and retinal pigment epithelial cells [40,56,60]. It has been also reported that in addition to trehalose other excipients are required to dry mammalian cells [38,49].…”

Section: Introductionmentioning

confidence: 99%

Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying

et al. 2019

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…At present, cell microencapsulation technology is considered to be a viable therapy. Microencapsulated somatic cells can be used to treat neurological and endocrine diseases, such as diabetes, syndromes after the removal of ovaries, and Parkinson's disease . Microencapsulated stem cells can aid bone regeneration and growth plate injury, repair the myocardium and improve angiogenesis .…”

Section: Introductionmentioning

confidence: 99%

The relationship between the inflammatory response and cell adhesion on alginate-chitosan-alginate microcapsules after transplantation

Shen

Zhang

Chen

et al. 2014

J. Biomed. Mater. Res.

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Cell microencapsulation technology is a potential alternative therapy, but cell overgrowth and adhesion on the microcapsules after transplantation shortens their time of therapeutic efficacy. Inflammatory cells were the main cells that adhered to the microcapsules, so understanding the body's inflammatory processes would help to better identify the mechanisms of cell adhesion to the outer surface of the microcapsules. Our study measured the inflammatory cells and the cytokines and characterized the associated changes in the alginate-chitosan-alginate (ACA) microcapsules 1, 7, 14, and 28 days after implantation in the peritoneal cavity. Then the relationship between the inflammatory response and cell adhesion on the microcapsules was evaluated by multiple regression analysis. The results showed that the microcapsules did not evoke a systemic inflammatory response, but initiated a local inflammatory response in the peritoneal cavity. Furthermore, the correlation analysis showed that the level of cell adhesion on the microcapsules was related to the number of lymphocytes and macrophages, and the amount of IL-6, IL-10, and MCP-1 in the peritoneal cavity. Our results may provide a foundation for reducing the immune response to these microcapsules, prolonging graft survival and improving the efficacy of these treatments.

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Viability of freeze dried microencapsulated human retinal pigment epithelial cells

Cited by 21 publications

References 35 publications

Trehalose effectiveness as a cryoprotectant in 2D and 3D cell cultures of human embryonic kidney cells

Trehalose effectiveness as a cryoprotectant in 2D and 3D cell cultures of human embryonic kidney cells

Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying

The relationship between the inflammatory response and cell adhesion on alginate-chitosan-alginate microcapsules after transplantation

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