Viability of Human Dental Pulp in Determination of Sex of an Individual by Identifying SRY Gene through DNA Analysis: A Single Blind Pilot Study

Naik, Prachi Ravikant; Acath, Deepa Das; Sharma, Gopal H; Navalkar, Amita; Kailasam, Senthilkumar

doi:10.5005/jp-journals-10011-1278

Cited by 7 publications

(9 citation statements)

References 12 publications

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“…Naik et al . [9] were able to retrieve the mean DNA quantity of 26.41 ng/μl, which on corelation with our study was significantly low. In our study, the freshly extracted teeth showed a wide range of DNA quantity.…”

Section: Discussionsupporting

confidence: 72%

Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction

Pawar

2018

J Forensic Dent Sci

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Introduction:In this fast era of numerous unwanted disasters and because of the severely devastated and degenerated body remains, personal identification of unknown remains has become the most difficult and challenging task. In such instances, dental pulp plays a vital role in identification through deoxyribonucleic acid (DNA).Aim:The aim of the study is to determine sex from tooth pulp tissues by DNA analysis using polymerase chain reaction amplification method under different environmental conditions.Materials and Methods:The human extracted teeth were exposed to different conditions such as heat, soil, and open environment. The DNA was extracted from all these teeth including freshly extracted teeth, then quantified, and further amplified with male and female primers.Results:Quantity of DNA content achieved ranged from 5.21 to 62.87 ng/μl. The accuracy in determining sex from pulp DNA ranged from 92% to 100% in the study groups, except from the teeth exposed to uncontrolled heat, as the pulp tissue was burnt completely. The intergroup analysis was statistically highly significant (P < 0.001). Gender determination using the quantity of DNA was found to be nonsignificant (P > 0.05).Conclusion:The dental pulp is the reliable source for sex determination in the humid or dry environment compared to uncontrolled heat.

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Section: Discussionsupporting

confidence: 72%

Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction

Pawar

2018

J Forensic Dent Sci

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“…Pulp which was retrieved was sufficient to amplify the DNA in the study. Naik et al 7 was able to retrieve the mean DNA quantity of 26.41 ng/μL. In our study, extracted teeth showed a wide range of DNA quantity.…”

Section: Discussionmentioning

confidence: 49%

Individuals Age Determination from Human Dental Pulp Through DNA Analysis by PCR

Tejasvi

Reddy

et al. 2021

Global Medical Genetics

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Objectives Age estimation in forensic odontology is having a great importance now a days because of the request by court or other government authorities so that immigrants whose real age is unknown should not suffer unfair disadvantages because of their supposed age, and so that all legal procedures to which an individual's age is relevant can be properly followed. Purpose The present study is planned to be conducted on pulp tissue and dental hard tissues derived from individuals for DNA isolation and age determination . Materials and Methods The present study was an experimental single-blinded study consisting of 30 extracted teeth categorized into three groups as follows: Group A: 10 to 20 years, Group B: 21 to 30 years, Group C: 31 to 40 years. DNA was isolated from the pulp of each tooth and quantitative polymerase chain reaction (qPCR) for calculating telomere length was performed. Results With increase in age, the length of telomere gets shortened which will be helpful in analyzing the age of the person when morphological and biological remnants are not available except the tooth. Conclusion The present study found that estimation of human age based on the relative TL measured by the real-time quantitative PCR may be a useful method for age prediction, especially when there is no morphologic information in the biological sample. This is the first study to accesses the age of a person by telomere length using dental pulp.

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“…In this regard, many studies have used the dental pulp for sex determination (Veeraraghavan et al, 2010;Zapico & Ubelaker, 2013;Nogami et al, 2008;Nayar et al, 2014;Naik et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Sex determination from dental pulp DNA among Egyptians

Kholief

El-Shanawany

Gomaa

2017

Egypt J Forensic Sci

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Background: A great challenge arises when sex determination of human remains is needed, especially when only, bone fragments or isolated teeth are available; such as in mass disasters. Thus, the aim of the present work is to extract and analyze the DNA from dental pulp for sex determination of a sample of Egyptian population using the polymerase chain reaction amplification of DYS14 and SRY genes. Materials and methods: DNA was extracted from the dental pulp of forty freshly extracted teeth (sound and carious) collected from adult Egyptians of both sexes using QIAamp® DNA Investigator Kit. Androgen Receptor gene was PCR amplified as an internal positive control. DYS14 and SRY genes were PCR amplified for sex determination. Results: DNA extraction was confirmed by detection of the amplified AR gene band (292 bp) in all samples. Sex was successfully determined for all the studied teeth as revealed by amplification of 158 bp band, 778 bp band of the DYS14 and SRY genes respectively. Conclusion: DYS14 and SRY genes were found to be reliable for dental sex determination regardless of the condition of teeth, whether sound or carious.

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Viability of Human Dental Pulp in Determination of Sex of an Individual by Identifying SRY Gene through DNA Analysis: A Single Blind Pilot Study

Cited by 7 publications

References 12 publications

Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction

Sex determination from tooth pulp deoxyribonucleic acid using polymerase chain reaction

Individuals Age Determination from Human Dental Pulp Through DNA Analysis by PCR

Sex determination from dental pulp DNA among Egyptians

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