Viability of
            <i>Trichomonas vaginalis</i>
            in Transport Medium

Beverly, A.; Venglarik, M.; Cotton, B.; Schwebke, Jane R.

doi:10.1128/jcm.37.11.3749-3750.1999

Cited by 23 publications

(12 citation statements)

References 11 publications

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“…One strategy for increasing diagnosis and treatment of trichomonosis is the use of a screening test with increased sensitivity compared to the traditional wet prep of vaginal fluid. Culture methods are currently the gold standard and should be considered for widespread clinical use (2,4). PCR techniques have proven superior to culture for other infections such as gonorrhea and chlamydia, and moreover urine has been found to be a suitable testing substrate for these techniques in men and women (7,32).…”

Section: Discussionmentioning

confidence: 99%

Detection of Trichomonosis in Vaginal and Urine Specimens from Women by Culture and PCR

2000

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Vaginal trichomonosis is a highly prevalent infection which has been associated with human immunodeficiency virus acquisition and preterm birth. Culture is the current “gold standard” for diagnosis. As urine-based testing using DNA amplification techniques becomes more widely used for other sexually transmitted diseases (STDs) such as gonorrhea and chlamydia, a similar technique for trichomonosis would be highly desirable. Women attending an STD clinic for a new complaint were screened for Trichomonas vaginalis by wet-preparation (wet-prep) microscopy and culture and for the presence of T. vaginalis DNA by specific PCR of vaginal and urine specimens. The presence of trichomonosis was defined as the detection of T. vaginalis by direct microscopy and/or culture from either vaginal samples or urine. The overall prevalence of trichomonosis in the population was 28% (53 of 190). The sensitivity and specificity of PCR using vaginal samples were 89 and 97%, respectively. Seventy-four percent (38 of 51) of women who had a vaginal wet prep or vaginal culture positive for trichomonads had microscopic and/or culture evidence of the organisms in the urine. Two women were positive for trichomonads by wet prep or culture only in the urine. The sensitivity and specificity of PCR using urine specimens were 64 and 100%, respectively. These results indicate that the exclusive use of urine-based detection of T. vaginalis is not appropriate in women. PCR-based detection of T. vaginalis using vaginal specimens may provide an alternative to culture.

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Section: Discussionmentioning

confidence: 99%

Detection of Trichomonosis in Vaginal and Urine Specimens from Women by Culture and PCR

2000

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“…Prior to NAATs, the gold standard for the laboratory diagnosis of TV was culture, using the InPouch™ TV (Biomed Diagnostics, White City, Oregon, USA). The sensitivity of the culture has been reported to be 81–94% [8, 9]. This method is time-consuming and not widely available for clinical use [6, 10].…”

Section: Introductionmentioning

confidence: 99%

Trichomonas vaginalis detection in female specimens with cobas® TV/MG for use on the cobas® 6800/8800 systems

Marlowe

Gohl²,

Steidle

et al. 2019

EuJMI

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Trichomoniasis, a common curable sexually transmitted infection caused by the protozoan Trichomonas vaginalis (TV), is usually asymptomatic. However, symptomatic women may experience vaginal discharge and/or vulvar irritation. This study evaluated cobas ® TV/ Mycoplasma genitalium (MG) (Conformité Européene marking for in vitro diagnostic medical devices [CE-IVD]) against other nucleic acid amplification tests (NAATs) for detecting TV in female urogenital specimens. Matched de-identified specimens from 412 females were collected. cobas ® TV/MG results were compared against a composite reference (CR) of 3 different NAATs for TV (Aptima TV, modified S-DiaMGTV™, and a laboratory-developed test). The overall TV prevalence rate was 6.2%, based on cobas ® TV/MG results. Relative to the CR, cobas ® TV/MG sensitivity/specificity for the specimen types were endocervical swabs (ES) 100%/99.2%, vaginal swabs (VS) 100%/99.7%, urine (U) 100%/99.7%, and cervical specimens in PreservCyt ® solution (PC) 100%/99.5%. There was no significant statistical difference between clinician-collected and self-collected VS ( p = 0.28). Correlation of cobas ® TV/MG vs. Aptima TV demonstrated the following positive, negative, and overall percent agreements, respectively: ES 69.0%, 98.7%, and 96.6%; VS 88.9%, 99.5%, and 98.8%; U 100%, 100%, and 100%; and PC 95.5%, 99.0%, and 98.8%. Detection of TV with cobas ® TV/MG for use on the cobas ® 6800/8800 systems demonstrated excellent performance in female urogenital specimens (overall sensitivity/specificity of 100%≥99.2%).

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“…Most studies used wet mount microscopy and/or TV culture to diagnose TV, likely due to older study enrollment dates and/or available resources in the clinical setting. Wet-mount microscopy has the poorest sensitivity (51–65%), 53,54 followed by culture (81–94%) for detection of TV 55,56 . Only 2 studies used TV NAATs with high sensitivity (95.3–100%) and specificity (95.2–100%) 57,58 .…”

Section: Discussionmentioning

confidence: 99%

Bacterial Vaginosis and Its Association With Incident Trichomonas vaginalis Infections: A Systematic Review and Meta-Analysis

et al. 2021

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Background: Bacterial vaginosis (BV) has been associated with an increased risk for acquisition of human immunodeficiency virus and sexually transmitted infections. We evaluated the association between BV and incident Trichomonas vaginalis (TV) infection in women.Methods: MEDLINE and ClinicalTrials.gov were searched for articles published between January 1, 1980, and May 7, 2021. Observational studies in women that evaluated the relationship between having/not having BV and the risk for acquiring TV were included.Results: Fourteen studies were included in the systematic review; 12 studies were included in meta-analyses involving 18,424 participants. Most studies used Nugent scoring to diagnose BV. For TV diagnosis, 12 studies used wet mount microscopy or culture, and 2 used nucleic acid amplification tests. There was diversity in the measures of association used, so an overall effect size could not be calculated. The majority of studies reported odds ratios, which showed an increased risk of incident TV among women with BV versus without BV (adjusted odds ratio, 1.87; 95% confidence interval, 1.45-2.40; P = 0.007). However, there were heterogeneity and potential confounding factors (eg, age, sexual partners) reported among studies.Conclusions: This systematic review and meta-analysis provide evidence for a nearly 2-fold higher risk for acquiring TV among women with BV compared with women without BV.

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Viability of Trichomonas vaginalis in Transport Medium

Cited by 23 publications

References 11 publications

Detection of Trichomonosis in Vaginal and Urine Specimens from Women by Culture and PCR

Detection of Trichomonosis in Vaginal and Urine Specimens from Women by Culture and PCR

Trichomonas vaginalis detection in female specimens with cobas® TV/MG for use on the cobas® 6800/8800 systems

Bacterial Vaginosis and Its Association With Incident Trichomonas vaginalis Infections: A Systematic Review and Meta-Analysis

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