Viability of microencapsulated Akkermansia muciniphila and Lactobacillus plantarum during freeze-drying, storage and in vitro simulated upper gastrointestinal tract passage

Marcial-Coba, Martín Sebastián; Cieplak, Tomasz; Cahú, Thiago Barbosa; Blennow, Andreas; Knøchel, Susanne; Nielsen, Dennis Sandris

doi:10.1039/c8fo01331d

Cited by 70 publications

(30 citation statements)

References 79 publications

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“…A number of studies have shown that the viability of probiotics can be improved by embedding them within microgels or other types of microcapsules (Gonzalez‐Ferrero et al., ; Lee, Ji, Lee, Choi, & Cho, ; Marcial‐Coba et al., ; Mawad, Helmy, Shalkami, Kathayat, & Rajashekara, ; Mirtic et al., ; Tiani, Yeung, McClements, & Sela, ; Yao et al., ; Yeung et al., ; Yeung, Ucok, Tiani, McClements, & Sela, ). Typically, these particles have dimensions that fall within the 1 to 1,000 μm range.…”

Section: Microencapsulation Of Probioticsmentioning

confidence: 99%

Progress in microencapsulation of probiotics: A review

Yao

Xie

et al. 2020

Comp Rev Food Sci Food Safe

328

160

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The potential health benefits of probiotics may not be realized because of the substantial reduction in their viability during food storage and gastrointestinal transit. Microencapsulation can be used to enhance the resistance of probiotics to unfavorable conditions. A range of oral delivery systems has been developed to increase the level of probiotics reaching the colon including embedding and coating systems. This review introduces emerging strategies for the microencapsulation of probiotics and highlights the key mechanisms of their stress–tolerance properties. Recent in vitro and in vivo models for evaluation of the efficiency of probiotic delivery systems are also reviewed. Encapsulation technologies are required to maintain the viability of probiotics during storage and within the human gut so as to increase their ability to colonize the colon. These technologies work by protecting the probiotics from harsh environmental conditions, as well as increasing their mucoadhesive properties. Typically, the probiotics are either embedded inside or coated with food‐grade materials such as biopolymers or lipids. In some cases, additional components may be coencapsulated to enhance their viability such as nutrients or protective agents. The importance of having suitable in vitro and in vivo models to evaluate the efficiency of probiotic delivery systems is also emphasized.

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Section: Microencapsulation Of Probioticsmentioning

confidence: 99%

Progress in microencapsulation of probiotics: A review

Yao

Xie

et al. 2020

Comp Rev Food Sci Food Safe

328

160

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“…Interestingly, the potential temperature effect associated with an oxygen exposure can be further assessed in data pertaining to the strategies explored to successfully protect and deliver viable functional A. muciniphila cells, which have been focused mainly on encapsulation systems. According to Marcial-Coba et al (2018, 2019b, the stability displayed when assessing different microencapsulation formulations was higher at refrigeration temperatures (4 • C). The researchers described two microencapsulation protocols based on conventional extrusion mechanism [ionotropic crosslinking biopolymer in hardening solution containing cations like Ca 2+ (de Prisco et al, 2015)] using A. muciniphila strain DSM22959.…”

Section: Akkermansiamentioning

confidence: 99%

“…The researchers described two microencapsulation protocols based on conventional extrusion mechanism [ionotropic crosslinking biopolymer in hardening solution containing cations like Ca 2+ (de Prisco et al, 2015)] using A. muciniphila strain DSM22959. In the first study the authors reported the application of xanthan/gellan gum polymeric matrix for cell immobilization with a subsequent freeze-drying step, in which they used various combinations of cryoprotective compounds (Marcial-Coba et al, 2018). The use of higher sugar cryoprotectants [sweetener agave syrup 10 % (w/v)] combined with xanthan/gellan gum matrix, in the form of freeze-dried microcapsules, provided a higher encapsulation efficiency (EE) (76.2%) when compared with the other cryoprotectants.…”

Section: Akkermansiamentioning

confidence: 99%

Commensal Obligate Anaerobic Bacteria and Health: Production, Storage, and Delivery Strategies

Andrade¹,

Almeida²,

Domingos³

et al. 2020

Front. Bioeng. Biotechnol.

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In the last years several human commensals have emerged from the gut microbiota studies as potential probiotics or therapeutic agents. Strains of human gut inhabitants such as Akkermansia, Bacteroides, or Faecalibacterium have shown several interesting bioactivities and are thus currently being considered as food supplements or as live biotherapeutics, as is already the case with other human commensals such as bifidobacteria. The large-scale use of these bacteria will pose many challenges and drawbacks mainly because they are quite sensitive to oxygen and/or very difficult to cultivate. This review highlights the properties of some of the most promising human commensals bacteria and summarizes the most up-to-date knowledge on their potential health effects. A comprehensive outlook on the potential strategies currently employed and/or available to produce, stabilize, and deliver these microorganisms is also presented.

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“…While most of the canonical probiotic bacteria can tolerate and even use some oxygen, most of the next-generation therapeutic bacteria are strict anaerobes. Hence, the encapsulation and viable delivery of these may be pivotal (Marcial-Coba et al, 2018;van der Ark et al, 2017a).…”

Section: Market Development and Production Of Health-promoting Bacteriamentioning

confidence: 99%

Biotechnology of health-promoting bacteria

Douillard

Vos

2019

Biotechnology Advances

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Over the last decade, there has been an increasing scientific and public interest in bacteria that may positively contribute to human gut health and well-being. This interest is reflected by the ever-increasing number of developed functional food products containing health-promoting bacteria and reaching the market place as well as by the growing revenue and profits of notably bacterial supplements worldwide. Traditionally, the origin of probiotic-marketed bacteria was limited to a rather small number of bacterial species that mostly belong to lactic acid bacteria and bifidobacteria. Intensifying research efforts on the human gut microbiome offered novel insights into the role of human gut microbiota in health and disease, while also providing a deep and increasingly comprehensive understanding of the bacterial communities present in this complex ecosystem and their interactions with the gut-liver-brain axis. This resulted in rational and systematic approaches to select novel health-promoting bacteria or to engineer existing bacteria with enhanced probiotic properties. In parallel, the field of gut microbiomics developed into a fertile framework for the identification, isolation and characterization of a phylogenetically diverse array of health-promoting bacterial species, also called next-generation therapeutic bacteria. The present review will address these developments with specific attention for the selection and improvement of a selected number of health-promoting bacterial species and strains that are extensively studied or hold promise for future food or pharma product development.

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Viability of microencapsulated Akkermansia muciniphila and Lactobacillus plantarum during freeze-drying, storage and in vitro simulated upper gastrointestinal tract passage

Abstract: Microencapsulated and subsequently freeze-dried cells showed acceptable storage stability and enhanced survival during in vitro upper gastrointestinal tract passage.

Cited by 70 publications

References 79 publications

Progress in microencapsulation of probiotics: A review

Progress in microencapsulation of probiotics: A review

Commensal Obligate Anaerobic Bacteria and Health: Production, Storage, and Delivery Strategies

Biotechnology of health-promoting bacteria

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