Viability of Preadipocytes In Vitro: The Influence of Local Anesthetics and pH

Abstract

Our data could partially explain the varying results after autogenous fat transfer.

Effect of N-Acetylcysteine on Adipose-Derived Stem Cell and Autologous Fat Graft Survival in a Mouse Model

“…To date, research has focused on optimizing lidocaine and epinephrine concentrations in existing tumescent solution formulations, [37][38][39] or reducing the mechanical stress associated with cannula size and negative pressure. 3,5,11 To date, few groups have focused on approaches to target oxidative stress specifically.…”

Coenzyme Q10 Does Not Enhance Preadipocyte Viability in an In Vitro Lipotransfer Model

“…No influence of lidocaine and adrenaline could be detected. However, in previous studies we were able to show that local anesthetics have a major impact on preadipocyte viability and differentiation in vitro [1,31]. Therefore, lidocaine caused immediate preadipocyte death, but if seeded at the same viable cell densities, lidocaine-treated cells proliferated normally resulting in dense cultures after 4 days of cultivation [1].…”

Adipocyte and preadipocyte viability in autologous fat grafts: comparing the water jet-assisted liposuction (WAL) and Coleman techniques

“…In the WAL technique, jets of water used to dislodge fat tissue may be accountable for damaging the adipocytes. Likewise, larger volumes of infiltrated solution used during WAL may produce a cytotoxic effect, contributing to poor preadipocyte survival [14].…”