Viability Studies with Mycoplasma Gallisepticum Under Different Environmental Conditions

Chandiramani, N. K.; Roekel, H. Van; Olesiuk, Olga M.

doi:10.3382/ps.0451029

Cited by 26 publications

(19 citation statements)

References 17 publications

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“…Similar report was demonstrated by Talha (2003) who recorded 36% MG infection in a flock containing 300 chickens in comparison to 33% in a flock containing 250 chickens. Highest infection rate in large scale flocks probably due to faulty in management and bio-security (Chandiramani et al, 1966).…”

Section: Resultsmentioning

confidence: 99%

Seroprevalence of <i>Mycoplasma gallisepticum</i> infection in chicken in the greater Rajshahi district of Bangladesh

Hossain

Ali²,

Haque³

1970

Bangl. J. Vet. Med.

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Section: Resultsmentioning

confidence: 99%

Seroprevalence of <i>Mycoplasma gallisepticum</i> infection in chicken in the greater Rajshahi district of Bangladesh

Hossain

Ali²,

Haque³

1970

Bangl. J. Vet. Med.

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“…However, the survival time of the organisms outside the host has not received as much attention. Studies have been carried out by a number of workers on the survival of avian mycoplasmas under conditions which aim to simulate natural field conditions (Chandiramani et al, 1966;Polak-Vogelzang, 1977;Simon et al, 1989;Shimizu et al, 1990). Mycoplasma synoviae (Ms) was included in only the last of these studies and Mycoplasma iowae (Mi) does not appear to have been investigated hitherto.…”

Section: Introductionmentioning

confidence: 99%

Investigations into the survival of Mycoplasma gallisepticum, Mycoplasma synoviae and Mycoplasma iowae on materials found in the poultry house environment

et al. 1994

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SUMMARYFollowing preliminary experiments to determine suitable methods for studying mycoplasma survival, suspensions of Mycoplasma gallisepticum (four strains), Mycoplasma synoviae (two strains) or Mycoplasma iowae (two strains) were seeded onto replicate samples of cotton, rubber, straw, shavings, timber, food, feathers and human hair. The organisms were also seeded onto human skin, ear and nasal mucosa. All samples were cultured for viability after 4, 8, 12 and 24 h, and then daily up to 6 days. The identity of recovered mycoplasmas was confirmed by indirect immunofluorescence. All three Mycoplasma species survived for the longest time on feathers with M. gallisepticum surviving between 2 and 4 days and M. synoviae 2 to 3 days. The type strain of M. iowae remained viable for 5 days on feathers, while the field strain was still viable at the end of the 6-day experiment. This strain also survived for at least 6 days on human hair and several other materials. M. gallisepticum survived on human hair up to 3 days and one recent field isolate also survived in the nose for 24 h. Survival times of the organisms were generally less on other materials although M. gallisepticum could be isolated from straw, cotton and rubber samples after 2 days.

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“…glucose, maltose, sucrose, lactose, and mannitol. The plates were examined under a stereoscope (30 x) after 6-day incubation at 37 C in candle jars (9). The sugar tubes were incubated for 3 weeks and examined weekly.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Mycoplasma and Acholeplasma from Apparently Healthy and Diseased (Infectious Sinusitis) Turkeys

Sayed

Chandiramani

Garg

1981

Microbiology and Immunology

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Investigation of 136 turkeys (24 manifesting infra-orbital sinusitis, 112 apparently healthy) resulted in isolation of 79 strains of Mycoplasma and 4 of Acholeplasma. By the disc growth inhibition test with 16 reference antisera of avian serogroups, 55 strains were identified serologically and 28 remained unidentified.Thirteen strains of Mycoplasma gallisepticum, I of M. meleagridis, and 2 of Acholeplasma laidlawii were isolated from turkey sinusitis whereas serogroups C (2), D (19), F (8), M. meleagridis (4), M. anatis (4), A. laidlawii (2), and 28 unidentified strains were isolated from apparently healthy turkeys. Three patterns were recognized on the basis of glucose, maltose, and sucrose, fermentation. The most frequent, pattern I, included 13 M. gallisepticum strains whereas 5 M. meleagridis strains belonged to fermentation pattern III. Isolates were also studied for reduction of tetrazolium, methylene blue, potassium tellurite, resistance to methylene blue and sodium taurocholate, and production of arginine deiminase and "film and spots." Inoculation of selected isolates into developing chick embryos revealed that 2 A. laidlawii strains were nonpathogenic and 13 M. gallisepticum, I serogroup D and 2 serogroup F strains were pathogenic, causing 50-100% mortality. In vitro antibiotic disc sensitivity tests indicated that rovamycin (solubilized spiramycin) may be recommended for turkey mycoplasmosis. Isolation of 2 A. laidlawii strains from turkey sinusitis and 4 M. anatis strains from apparently healthy turkeys appears interesting.

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Viability Studies with Mycoplasma Gallisepticum Under Different Environmental Conditions

Cited by 26 publications

References 17 publications

Seroprevalence of <i>Mycoplasma gallisepticum</i> infection in chicken in the greater Rajshahi district of Bangladesh

Seroprevalence of <i>Mycoplasma gallisepticum</i> infection in chicken in the greater Rajshahi district of Bangladesh

Investigations into the survival of Mycoplasma gallisepticum, Mycoplasma synoviae and Mycoplasma iowae on materials found in the poultry house environment

Isolation and Characterization of Mycoplasma and Acholeplasma from Apparently Healthy and Diseased (Infectious Sinusitis) Turkeys

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