Vibration Analyses of a Single Osteoblast in Vitro using the Finite Element Method

Wang, Liping; Hsu, Hung-Yao; Chen, Xian

doi:10.2991/bbe-16.2016.58

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…Additionally, this experimental setup can be used for application of vibration in higher frequencies, i.e., above the infrasonic range . There are several previous reports that human osteoblast in vitro can be stimulated by the high-frequency vibration frequencies [4,7,8], probably by mimicking the natural influence of resting muscle on an attached bone, as it is seen on the vibromyogram [9] following muscle's spontaneous periodic contraction in this range of frequencies. These reports are also supported by the in vivo studies that showed an enhancing effect of vibration in this range of frequencies on the bone mass buildup [10].…”

Section: Introductionmentioning

confidence: 99%

Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration

Rosenberg¹,

Rosenberg²,

Politch³

et al. 2021

Iberoam J Med

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Introduction: Biomechanical stimulation of cultured human osteoblast-like cells, which is based on controlled mechanical vibration, has been previously indicated, but the exact mechanical parameters that are effective for cells' proliferation enhancement are still elusive due to the lack of direct data recordings from the stimulated cells in culture. Therefore, we developed a low friction tunable system that enables recording of a narrow range of mechanical parameters, above the infrasonic spectrum, that applied uniformly to human osteoblast-like cells in monolayer culture, aiming to identify a range of mechanical parameters that are effective to enhance osteoblast proliferation in vitro. Methods: Human osteoblast-like cells in explant monolayer culture samples were exposed to mechanical vibration in the 10-70Hz range of frequencies for two minutes, in four 24 hours intervals. Cell numbers in culture, cellular alkaline phosphatase activity (a marker of cell maturation), and lactate dehydrogenase activity in culture media (representing cell death) were measured after the mechanical stimulation protocol application and compared statistically to the control cell cultures kept in static conditions. The cell proliferation was deduced from cell number in culture and cell death measurements. Results: We found that 50-70 Hz of vibration frequency protocol (10-30 μm of maximal displacement amplitude, 0.03g of peak-to-peak acceleration) is optimal for enhancing cells' proliferation(p<0.05), with a parallel decrease of their maturation (p<0.01). Discussion: We detected the optimal mechanical parameters of excitation protocol for induction of osteoblast proliferation in vitro by a mechanical platform, which can be used as a standardized method in the research of mechanotransduction in human osteoblast.

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Section: Introductionmentioning

confidence: 99%

Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration

Rosenberg¹,

Rosenberg²,

Politch³

et al. 2021

Iberoam J Med

View full text Add to dashboard Cite

show abstract

“…There are several previous reports that human osteoblast in vitro can be stimulated in the infrasonic range of vibration frequencies [3,6,7], probably by mimicking the natural influence of resting muscle on an attached bone, as it is seen on the vibromyogram [8] following muscle's spontaneous periodic contraction in this range of frequencies. These reports are also supported by the in vivo studies that showed an enhancing effect of vibration in this range of frequencies on the bone mass buildup [9].…”

mentioning

confidence: 99%

Determination of biophysical parameters for enhancement of human osteoblast proliferation by mechanical vibration in the infrasonic frequency range

Rosenberg

Politch

Rosenberg³

et al. 2018

Preprint

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Experimental methods for studying an enhancement of osteoblast proliferation in vitro provide tools for the research of biochemical processes involved in bone turnover in vivo. Some of the current methods used for this purpose are based on the ability of the osteoblasts to enhance proliferation by mechanical stimulation. We describe an experimental approach of biomechanical stimulation of cultured human osteoblast-like cells by vibration. This method is based on the specially designed controlled vibration setup that consists of an electric actuator, with horizontally mounted well plate containing cell cultures. Previously this method found to be effective to enhance cell proliferation, but the exact mechanical parameters of effective vibration were elusive.The current low friction system for mechanical stimulation of osteoblast-like cells in vitro provides recording of narrow range mechanical parameters in the infrasonic spectrum.We exposed human osteoblast-like cells in explant monolayer culture to mechanical vibration in the 10-70Hz range of frequencies and found that 50-70 Hz of vibration frequency is optimal for inducing osteoblast proliferation that was deduced from interrelation between unchanged cell number in culture samples with significant decrease in cell death rate (decreased LDH activity in culture media, p<.05) and with parallel decrease of their maturation level (p<.01).In this report we determined the optimal mechanical parameters and excitation protocol for induction of osteoblast proliferation in vitro by using a tunable and versatile mechanical platform, which can be used in the research of cell mechanotransduction .

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Vibration Analyses of a Single Osteoblast in Vitro using the Finite Element Method

Cited by 2 publications

References 11 publications

Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration

Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration

Determination of biophysical parameters for enhancement of human osteoblast proliferation by mechanical vibration in the infrasonic frequency range

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