Abstract. The aims of our research work were the investigation of postharvest changes of pear samples (Pyrus communis cv. Bosc kobak) during combined cold storage and shelf-life (storage at room temperature), the determination of quality changes by mainly non-destructive methods, the modeling of the changes of the non-destructive parameters (acoustic, impact stiffness coefficient, chlorophyll fluorescence parameters [Fv/Fm, Fm/F0]), and multivariate statistical analysis of the measured and predicted data based on the data of the non-destructive texture analysis (acoustic and impact methods), chlorophyll fluorescence analysis and laser scattering measurement. Storage Time Equivalent Value (STEV) was calculated and introduced based on mass-loss analysis. The changes of the non-destructive parameters were analyzed vs. this virtual storage time (STEV). The changes of acoustic, impact stiffness coefficient and chlorophyll fluorescence parameters can be predicted by exponential function. The predicted time constants of the parameters were 21.0, 45.8, 47.1, 83.4, acoustic, impact stiffness coefficient, Fm/F0, Fv/Fm, respectively. The lower the time constant, the quicker is the change of the given parameter during storage, the higher is its sensitivity. By this point of view, the percentage mass loss related sensitivity to the characterization of textural changes, the predicted acoustic stiffness coefficient was found to be more sensitive than the impact stiffness coefficient. The Fm/F0 value characterized more sensibly the changes of the chlorophyll fluorescence than in the literature commonly used Fv/Fm. The non-contact laser scattering method based significant PLS models were constructed to predict the quality related pear characteristics (mechanical properties, chlorophyll fluorescence parameters).