In this paper, we mainly aimed to design a reduced-order extended state observer-based active vibration controller for a structural vibration control system with total disturbances, i.e., model uncertainties, higher harmonics, and external excitations. A reduced-order extended state observer (RESO)-based nonsingular terminal sliding mode vibration control (RESO–NTSMVC) method is proposed for the vibration suppression of an all-clamped plate structure with an inertial actuator. First, a second-order state space model of the thin plate, with an inertial actuator, was established by solving the dynamic partial differential equation and analyzing the physical model. Second, the total disturbances, i.e., model uncertainties, higher harmonics, and external excitations, were estimated and compensated for by using a RESO via a feedforward part. Third, a NTSMVC based on an estimated value was designed to obtain a fast-tracking rate and effective vibration suppression performance. In addition, the stability of the closed-loop system was proven by using a Lyapunov stability criterion. Finally, a semi-physical experimental instrument was built based on the MATLAB/Simulink real-time environment and the NI-PCIE6343 acquisition card to verify strong anti-disturbance performance and effective vibration control performance of the designed method. The experimental comparison results showed that the vibration amplitudes of the proposed method could be reduced by 11.7 dB, when the traditional extended state observer-based nonsingular terminal sliding mode vibration control (ESO–NTSMVC) method achieved a control effect of only 6.5 dB. The comparative experimental results showed that the proposed method possessed better vibration suppression performance and anti-disturbance performance.