Rotated blades are key mechanical components in turbine and high cycle fatigues often induce blade cracks. Meanwhile, mistuning is inevitable in rotated blades, which often makes it much difficult to detect cracks. In order to solve this problem, it is important and necessary to study effects of crack on vibration characteristics of mistuned rotated blades (MRBs). Firstly, a lumped-parameter model is established based on coupled multiple blades, where mistuned stiffness with normal distribution is introduced. Next, a breathing crack model is adopted and eigenvalue analysis is used in coupled lumped-parameter model. Then, numerical analysis is done and effects of depths and positions of a crack on natural frequency, vibration amplitude, and vibration localization parameters are studied. The results show that a crack causes natural frequency decease and vibration amplitude increase of cracked blade. Bifurcations will occur due to a breathing crack. Furthermore, based on natural frequencies and vibration amplitudes, variational factors are defined to detect a crack in MRBs, which are validated by numerical simulations. Thus, the proposed method provides theoretical guidance for crack detection in MRBs.