Fatigue in steel–concrete composite beams can result from cyclic loading, causing stress fluctuations that may lead to cumulative damage and eventual failure over an extended period. In this paper, the experimental findings from fatigue loading tests on composite beams with various arrangements are presented. Fatigue tests were performed up to 1,000,000 cycles using four-point loading, encompassing various ranges of shear stress at a consistent amplitude. Additionally, the effects of external post-tensioning and the strength of the shear connection were investigated. Static tests were run until failure to assess the enduring strength of the specimens subjected to fatigue. The cyclic mid-span deflections, slippages, and strains were measured during the testing. Based on the experimental findings, it was found that the damage region that the shear studs caused in the concrete slab, which resulted in a reduction in stiffness within the shear connection, grew as the loading cycles increased, leading to an increase in residual deflections and plastic slippages. Controlling the longitudinal fatigue cracks in the concrete slab was largely dependent on the strength of the shear connection between the steel beams and concrete slabs. Moreover, the applied fatigue loading range affected the propagation and distribution of fatigue cracks in the concrete slab. The strains in different parts of the composite specimens were significantly reduced by applying the external post-tensioning. With no signs of distress at the anchors, the tendons displayed excellent fatigue performance.