The complex mechanical system of a rice combine harvester not only has various excitation sources, but also, the vibration transmission path between each working device and the vibration contribution characteristics to the frame are not clear, so it is difficult to perform a reduction vibration design for the sharp vibration of the rice combine harvester frame. Therefore, based on the comparison and improvement of multiple classical transfer path analysis methods, this paper analyzed the vibration transfer characteristics and transfer characteristics of each harvester by the discrete time matrix method and operating path method. In the Experimental section, through the vibration characteristic experiment firstly, this paper obtained the power spectrum variation and the most needed optimized path in the transmission path of each device under each operating condition. Secondly, through frame simulation analysis under the exciting force, we obtained the vibration damping areas that needs to be optimized. Finally, the damping optimization experiment connected with the vibration characteristic experiment, and the excitation force simulation analysis was performed. The results of the damping optimization experiment displayed that the maximum change value of the vibration acceleration of the cutting table decreased from 7.862 m·s−2 to 3.522 m·s−2, decreasing by 55.2%, and the peak amplitude of the multipoint test in the cab was 5.4, 5.3, 1.7 and 2.0 μm, respectively, which was significantly reduced, so the optimization effect was significant. This study provides theoretical support for the vibration reduction optimization of a rice combine harvester frame.