In the present study, author analyses the vibration of protein microtubules and its interaction with the surrounding elastic medium and electromagnetic and gravitational forces. Protein microtubules are dynamic Nano fibers found inside the cell’s cytoplasm, strengthening the cytoskeleton and gives shape to the cell. When the cell undergoes deformation microtubules vibrate. Understanding the vibration of these Nano fibers within the elastic medium and along with the body forces as electromagnetic and gravitational forces is crucial. The main purpose of this study is to explore the effects of surrounding elastic medium and electromagnetic and gravitational forces on the vibration of protein microtubules. The findings reveal that the effects of surrounding medium and the external influences on the vibration of these Nano fibers are significant. The study presents the numerical values in the form of table associated with microtubules in various conditions: free, embedded, and in the presence of electromagnetic and gravitational forces.