Vibrational circular dichroism of d‐amino acid‐containing peptide NdWFamide in the crystal form

Abstract

Microcrystals of L-Asn-D-Trp-L-Phe-NH 2 (NdWFamide), a tripeptide derived from Aplysia kurodai that exhibits invertebrate cardiac activity, were evaluated by vibrational circular dichroism (VCD). The chirality of the tryptophan residue at the second position in NdWFamide was associated with the conformation and biological characteristics. The VCD spectrum of NdWFamide was a mirror image of its enantiomer; however, it was significantly different from that of its diastereomer, NWFamide, which is its precursor. The obtained VCD signals of NdWFamide were in good agreement with the VCD signals that were calculated based on the optimized aggregates of NdWFamide, which formed a helical-like backbone conformation. The evaluation of the VCD results revealed the conformation of NdWFamide in the crystalline state and succeeded in distinguishing its stereoisomers. Therefore, this study demonstrates VCD as a useful method for the structural analysis of naturally occurring D-amino acid-containing peptides. K E Y W O R D Samides I and II, chirality, D-amino acid-containing peptide, right-handed helix, vibrational circular dichroism spectroscopy