Vibrational Optical Activity of Amyloid Fibrils

Abstract

Amyloid fibrils are supramolecular systems showing distinct chirality at different levels of their complex multilayered architectures. Due to the regular long‐range chiral organization, amyloid fibrils exhibit the most intense Vibrational Optical Activity (VOA) signal observed up to now, making VOA techniques: Vibrational Circular Dichroism (VCD) and Raman Optical Activity (ROA) very promising tools to explore their structures, handedness and intricate polymorphism. This concept article reviews up‐to‐date experimental studies on VOA application to investigate amyloid fibrils highlighting its future potential in analyzing of these unique supramolecular systems, in particular in the context of biomedicine and nanotechnology.

Microscopic vibrational circular dichroism on the forewings of a European hornet: heterogenous sequences of protein domains with different secondary structures

“…The secondary structure of the protein at each circle was identified by comparing the obtained VCD spectra with those reported for authentic samples of various secondary strucutures. 37–48 From this comparison, we deduced that the domains change from β-sheet (green circle) to α-helix (red circle), and then return to β-sheets (black circle) (Fig. 3(c)).…”

Automated microscopic measurement of fibrinaloid microclots and their degradation by nattokinase, the main natto protease

“…This was then followed by immediately pipetting 15 μL of assay sample on 15-well slide 'angiogenesis' glass bottom plate used without a lid (I https://ibidi.com/chambered-coverslips/245--slide-15-well-3d-glass-bottom.html), reproduced in Figure 3, and wit shaking (cf. [142,[297][298][299][300][301][302][303][304][305][306][307][308][309]). The excitation wavelength band for ThT was set at 450 nm to 499 nm and the emissio 499 nm to 529 nm.…”