Vibrational Spectroscopic Imaging of Soft Tissue

Krafft, Christoph; Popp, Jürgen

doi:10.1002/9783527678136.ch3

Cited by 12 publications

(10 citation statements)

References 85 publications

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“…In the case of QCL-based measurements, this might hint at the distribution of highly glycosylated peptides. 24 Spectral differences in the region of 1000 to 1100 cm −1 have already been used to identify malignant colon tissue epithelium using FT-IR mapping with a large sample size but at low resolution. 4 Future experiments will be required to verify whether adenocarcinomas in colon tissue can be detected using the QCL-based setup.…”

Section: Resultsmentioning

confidence: 99%

Quantum cascade laser–based hyperspectral imaging of biological tissue

et al. 2014

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Abstract. The spectroscopy of analyte-specific molecular vibrations in tissue thin sections has opened up a path toward histopathology without the need for tissue staining. However, biomedical vibrational imaging has not yet advanced from academic research to routine histopathology due to long acquisition times for the microscopic hyperspectral images and/or cost and availability of the necessary equipment. Here we show that the combination of a fast-tuning quantum cascade laser with a microbolometer array detector allows for a rapid image acquisition and bares the potential for substantial cost reduction. A 3.1 × 2.8 mm 2 unstained thin section of mouse jejunum has been imaged in the 9.2 to 9.7 μm wavelength range (spectral resolution ∼1 cm −1 ) within 5 min with diffraction limited spatial resolution. The comparison of this hyperspectral imaging approach with standard Fourier transform infrared imaging or mapping of the identical sample shows a reduction in acquisition time per wavenumber interval and image area by more than one or three orders of magnitude, respectively. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Resultsmentioning

confidence: 99%

Quantum cascade laser–based hyperspectral imaging of biological tissue

et al. 2014

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“…Moreover, a wider range of sample types can be analyzed simply and without any special preparation, including aqueous solutions, fibers, powders, or frozen materials [22]. Recently, Raman spectroscopy has been explored for use in biomedical applications, because it can provide detailed information on the chemical composition of cells and tissues [29]. Fig.…”

Section: Micro-raman Spectroscopy Of Hard Tissuesmentioning

confidence: 99%

Studies on bone metabolism by using isotope microscopy, FTIR imaging, and micro-Raman spectroscopy

Kimura-Suda

Kajiwara

Sakamoto

et al. 2013

Journal of Oral Biosciences

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“…High axial resolutions are obtained by confocal microscopes. Second advantage is achieving maximum sensitivity with a maximum photon flux of laser beam onto the sample and the maximum collection of scattered photons from the sample [20,28]. Briefly, Raman microscopy can either be operated in a point-detection mode, scanning of wide field mode with its high spatial resolution and signal-to-noise ratio enable the single cells to collect their spectra from focal volumes smaller than 1 μm [29].…”

Section: Fourier Transform Infrared (Ftir) Spectroscopymentioning

confidence: 99%

“…Raman imaging of cells complementary with analytical methods in cell biology like electron microscopy, fluorescence microscopy, and autoradiography which require complicated preparations and manipulations before they can be applied. Raman imaging can overcome problems resulting from the limited stability, bleaching, and restricted accessibility of external markers [28]. Band assignments of Raman spectra of human cell lines are presented in Table 2, according to different publications in the literature.…”

Section: Fourier Transform Infrared (Ftir) Spectroscopymentioning

confidence: 99%

Role of Vibrational Spectroscopy in Stem Cell Research

Aksoy¹,

Severcan

2012

Spectroscopy: An International Journal

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Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the stem cells have some unwanted effects on the properties of stem cells, and these methods also do not provide real-time information about cellular conditions. These challenges enforce the usage of nondestructive, rapid, sensitive, high quality, label-free, cheep, and innovative chemical monitoring methods. In this context, vibrational spectroscopy provides promissing alternative to get new information into the field of stem cell biology for chemical analysis, quantification, and imaging of stem cells. Raman and infrared spectroscopy and imaging can be used as a new complimentary spectroscopic approaches to gain new insight into stem cell reseaches for future therapeutic and regenerative medicines. In this paper, recent developments in applications of vibrational spectroscopy techniques for stem cell characterization and identification are presented.

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Vibrational Spectroscopic Imaging of Soft Tissue

Cited by 12 publications

References 85 publications

Quantum cascade laser–based hyperspectral imaging of biological tissue

Quantum cascade laser–based hyperspectral imaging of biological tissue

Studies on bone metabolism by using isotope microscopy, FTIR imaging, and micro-Raman spectroscopy

Role of Vibrational Spectroscopy in Stem Cell Research

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