Vibrio alginolyticus Survives From Ofloxacin Stress by Metabolic Adjustment

Yin, Yue; Yin, Yuanpan; Yang, Hao; Chen, Zhuang‐Gui; Zheng, Jun; Peng, Bo

doi:10.3389/fmicb.2022.818923

Cited by 19 publications

(15 citation statements)

References 52 publications

(68 reference statements)

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“…qRT-PCR was performed as previously described [ 19 , 66 ]. Total RNA was isolated from GBS with TRIzol (Invitrogen Life Technologies, United States) after treatment with lysozyme for 30 min at 37℃.…”

Section: Methodsmentioning

confidence: 99%

Fructose promotes ampicillin killing of antibiotic-resistant Streptococcus agalactiae

Chen

Liu

et al. 2023

Virulence

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Streptococcus agalactiae (GBS) is an important pathogenic bacteria that infected both aquatic animals and human beings, causing huge economic loss. The increasing cases of antibiotic-resistant GBS impose challenges to treat such infection by antibiotics. Thus, it is highly demanded for the approach to tackle antibiotic resistance in GBS. In this study, we adopt a metabolomic approach to identify the metabolic signature of ampicillin-resistant GBS (AR-GBS) that ampicillin is the routine choice to treat infection by GBS. We find glycolysis is significantly repressed in AR-GBS, and fructose is the crucial biomarker. Exogenous fructose not only reverses ampicillin resistance in AR-GBS but also in clinic isolates including methicillin-resistant Staphylococcus aureus (MRSA) and NDM-1 expressing Escherichia coli . The synergistic effect is confirmed in a zebrafish infection model. Furthermore, we demonstrate that the potentiation by fructose is dependent on glycolysis that enhances ampicillin uptake and the expression of penicillin-binding proteins, the ampicillin target. Our study demonstrates a novel approach to combat antibiotic resistance in GBS.

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Section: Methodsmentioning

confidence: 99%

Fructose promotes ampicillin killing of antibiotic-resistant Streptococcus agalactiae

Chen

Liu

et al. 2023

Virulence

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“…The depression of the P cycle led to the resistance of Vibrio alginolyticus to gentamicin (Zhang et al, 2019). The inactivation of the P cycle and the fatty acid biosynthesis promoted the resistance of Vibrio alginolyticus to ofloxacin (Yin et al, 2022). Secondly, two aspects of experiments were conducted to verify that the depressed P cycle was associated with the KA resistance in Xoo.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Alanine-mediated P cycle boosting enhances the killing efficiency of kasugamycin on antibiotic-resistant Xanthomonas oryzae

et al. 2023

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The outbreak of Bacterial blight (BB) caused by Xanthomonas oryzae (Xoo) generates substantial economic losses to agricultural production. Antibiotics application is a valuable measure to control this bacterial disease. However, microbial antibiotic resistance dramatically reduced antibiotic effectiveness. Identifying the resistance mechanism of Xoo to antibiotics and restoring antibiotic susceptibility is one of the crucial ways to solve this problem. This study employed a GC-MS-based metabolomic approach to reveal the differential metabolomics between a kasugamycin-susceptible Xoo strain (Z173-S) and a kasugamycin-resistant strain (Z173-RKA). The metabolic mechanism of kasugamycin (KA) resistance in Xoo by GC–MS showed that the downregulation of the pyruvate cycle (P cycle) is a crucial feature of Z173-RKA resistance to KA. This conclusion was confirmed by the decreased enzyme activities and the related gene transcriptional level in the P cycle. Furfural (an inhibitor of pyruvate dehydrogenase) can effectively inhibit the P cycle and increase the resistance of Z173-RKA to KA. Moreover, exogenous alanine can reduce the resistance of Z173-RKA to KA by promoting the P cycle. Our work seems to be the first exploration of the mechanism of KA resistance in Xoo by GC–MS-based metabonomics approach. These results provide a new idea for developing metabolic regulation to address KA resistance in Xoo.

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“…The activity of PDH was measured as reported (75)(76)(77), and the PK enzymatic activity was assessed using a commercial assay kit (BC0545, Beijing Solarbio Science and Technology Co., Ltd.). The bacteria were treated as stated above.…”

Section: Measurement Of Enzyme Activitymentioning

confidence: 99%

Functional proteomics identify mannitol metabolism in serum resistance and therapeutic implications in Vibrio alginolyticus

Kou

Chen

et al. 2022

Front. Immunol.

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Serum resistance is recognized as one of the most important pathogenic traits of bacterial pathogens, and no control measure is available. Based on our previous discovery that pathogenic Escherichia coli represses glycine, serine, and threonine metabolism to confer serum resistance and that the reactivation of this pathway by exogenous glycine could restore serum sensitivity, we further investigate the mechanism underlying the action of glycine in Vibrio alginolyticus. Thus, V. alginolyticus is treated with glycine, and the proteomic change is profiled with tandem mass tag-based quantitative proteomics. Compared to the control group, glycine treatment influences the expression of a total of 291 proteins. Among them, a trap-type mannitol/chloroaromatic compound transport system with periplasmic component, encoded by N646_0992, is the most significantly increased protein. In combination with the pathway enrichment analysis showing the altered fructose and mannitol metabolism, mannitol has emerged as a possible metabolite in enhancing the serum killing activity. To demonstrate this, exogenous mannitol reduces bacterial viability. This synergistic effect is further confirmed in a V. alginolyticus–Danio rerio infection model. Furthermore, the mechanism underlying mannitol-enabled serum killing is dependent on glycolysis and the pyruvate cycle that increases the deposition of complement components C3b and C5b-9 on the bacterial surface, whereas inhibiting glycolysis or the pyruvate cycle significantly weakened the synergistic effects and complement deposition. These data together suggest that mannitol is a potent metabolite in reversing the serum resistance of V. alginolyticus and has promising use in aquaculture.

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Vibrio alginolyticus Survives From Ofloxacin Stress by Metabolic Adjustment

Cited by 19 publications

References 52 publications

Fructose promotes ampicillin killing of antibiotic-resistant Streptococcus agalactiae

Fructose promotes ampicillin killing of antibiotic-resistant Streptococcus agalactiae

Alanine-mediated P cycle boosting enhances the killing efficiency of kasugamycin on antibiotic-resistant Xanthomonas oryzae

Functional proteomics identify mannitol metabolism in serum resistance and therapeutic implications in Vibrio alginolyticus

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