Vibrio cholerae O139 synonym bengal is closely related to Vibrio cholerae El Tor but has important differences

Johnson, J. A.; Ca, Salles; Panigrahi, Pinaki; Albert, M. John; Wright, AC; Johnson, Roy A.; Morris, Joel

doi:10.1128/iai.62.5.2108-2110.1994

Cited by 156 publications

(69 citation statements)

References 10 publications

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“…Vibrio cholerae O1 El Tor biotype as well as O139 serogroup possess mannose-sensitive haemagglutinin (MSHA), which is required for colonization to zooplankton (Chiavelli et al, 2001). However, it is well documented that V. cholerae O139 possesses a capsular polysaccharide and a different lipopolysaccharide, whereas O1 strains do not (Johnson et al, 1994;Knirel et al, 1995Knirel et al, , 1997.…”

Section: Introductionmentioning

confidence: 99%

Vibrio cholerae O1 strains are facultative intracellular bacteria, able to survive and multiply symbiotically inside the aquatic free-living amoeba Acanthamoeba castellanii

Abd

Saeed

Weintraub

et al. 2007

FEMS Microbiology Ecology

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Vibrio cholerae species are extracellular, waterborne, gram-negative bacteria that are overwhelmed by predators in aquatic environments. The unencapsulated serogroup V. cholerae O1 and encapsulated V. cholerae O139 cause epidemic and pandemic outbreaks of cholera. It has recently been shown that the aquatic and free-living amoeba Acanthamoeba castellanii is not a predator to V. cholerae O139; rather, V. cholerae O139 has shown an intracellular compatibility with this host. The aim of this study was to examine the ability of V. cholerae O1 classical and El Tor strains to grow and survive in A. castellanii. The interaction between A. castellanii and V. cholerae O1 strains was studied by means of amoeba cell counts and viable counts of the bacteria in the absence or presence of amoebae. The viable count of intracellularly growing bacteria was estimated by utilizing gentamicin assay. Confocal microscopy and electron microscopy were used to determine the intracellular localization of V. cholerae in A. castellanii. The results showed that V. cholerae O1 classical and El Tor strains grew and survived intracellularly in the cytoplasm of trophozoites, and that the bacteria were also found in the cysts of A. castellanii. The interaction showed a facultative intracellular behaviour of V. cholerae O1 classical and El Tor strains and a possible role of A. castellanii as an environmental host of V. cholerae species.

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Section: Introductionmentioning

confidence: 99%

Vibrio cholerae O1 strains are facultative intracellular bacteria, able to survive and multiply symbiotically inside the aquatic free-living amoeba Acanthamoeba castellanii

Abd

Saeed

Weintraub

et al. 2007

FEMS Microbiology Ecology

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“…Outer-membrane lipopolysaccharide (LPS) of both 0 1 serotypes, Inaba and Ogawa, have a long 0-side chain identified as a homopolymer of 4-(3-deoxy-~-glycero-tetronamido)-4,6-dideoxy-~-mannose [4, 51 terminated in the latter serotype with the 2-0-methyl derivative of the same monosaccharide [6]. In contrast, K cholerae 0139 produces a polysaccharide capsule [2,71 and has an LPS with a short 0-side chain [7-91. Serological and genetic studies suggested that in V cholerae 01 We now report structural characterization, including data on both the 0-side chain and the core-lipid A region, of the LPS from V cholerue 0139 CPS-free mutant strains M010-T4 with the complete LPS and Bengal-2R with a truncated LPS. The preliminary results were presented at the XVIIIth International Carbohydrate Symposium (Milan, July 1996) [I 81.…”

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confidence: 99%

Structural Studies on the Short‐Chain Lipopolysaccharide of Vibrio Cholerae O139 Bengal

Knirel

Widmalm

Senchenkova

et al. 1997

European Journal of Biochemistry

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A Vibrio cholerae 0 1 39 strain, M010-T4, lacking capsular polysaccharide, produces a short-chain lipopolysaccharide (LPS), similar to enterobacterial SR strains. It was studied by acidic and alkaline degradation, dephosphorylation, sugar and methylation analysis, high-performance anion-exchange chromatography, one-and two-dimensional 'H-, ' T -, and "P-NMR spectroscopy, and electrospray ionization mass spectrometry. The following structure was proposed for the core region of the LPS:where PEtn stands for 2-aminoethyl phosphate, Fru for fructose, Hep for L,-glycero-D-manno-heptose, and Kdo for 3-deoxy-D-munno-octulosonic acid; unless otherwise stated, the monosaccharide residues are D and present in the pyranose form. An 0-acetyl group is present on a secondary position, tentatively 0 4 of the a-linked glucosyl group. Some LPS species contain an additional putative fructose residue whose location remains unknown. An 0139-negative mutant strain, Bengal-2R, derived from V cholerae 0139, has also been investigated and shown to produce an 0-antigen-lacking LPS similar to those from enterobacterial R strains, some of the LPS species containing the same core region as the strain M010-T4 LPS and the other lacking the lateral heptose residue. The carbohydrate backbone core structure is the same for the L! cholerae 0139 and L! cholerae 0 1 LPS, thus confirming the close relation between these bacteria; however, the 2-aminoethyl phosphate, the 0-acetyl group, and the second fructose residue have not been reported for the 0 1 LPS. In the V cholerue 0139 strain M010-T4 LPS, a short 0-side chain is attached at position 3 of the 7-substituted heptose residue and has the same structure as one repeating unit of the L! cholerue 0139 capsular polysaccharide. Some details of the structure proposed are at variance with those recently published for another I/: cholerae 0139 strain

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“…The disease affected mainly adults suggesting that, despite the epidemic in this area a year or more earlier, the population continued to be immunologically naive. The organism is itself considered to be closely related to Vibrio cholerae El Tor, with additional virulence factors (Calia et al 1994;Johnson et al 1994). The disease produced by this organism is clinically indistinguishable from classic cholera (Bhattacharya et al 1993) and the diagnosis in this study was made by culture and serotyping with 0 7 3 9 antiserum.…”

Section: Discussionmentioning

confidence: 91%

Isolation of Vibrio cholerae O139 from the drinking water supply during an epidemic of cholera

Ramakrishna

Kang

Rajan

et al. 1996

Tropical Med Int Health

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In mid-1994, the public water supply was investigated in a medium-sized town in south India during an epidemic of cholera due to Vibrio cholerae 0 1 3 9 . Vibrio cholerae 0 1 3 9 was isolated from the public water supply including one of the wells supplying the town, the central overhead tank, and domestic taps connected to the public supply. Following chlorination, the organism was no longer isolated from the water supply and the epidemic subsided. This demonstration of V. cholerae 0 1 3 9 in the drinking water supply of a town underlines the need for adequate treatment of the water supply. keywords cholera, Vibrio cholerae 0 1 3 9

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Vibrio cholerae O139 synonym bengal is closely related to Vibrio cholerae El Tor but has important differences

Cited by 156 publications

References 10 publications

Vibrio cholerae O1 strains are facultative intracellular bacteria, able to survive and multiply symbiotically inside the aquatic free-living amoeba Acanthamoeba castellanii

Vibrio cholerae O1 strains are facultative intracellular bacteria, able to survive and multiply symbiotically inside the aquatic free-living amoeba Acanthamoeba castellanii

Structural Studies on the Short‐Chain Lipopolysaccharide of Vibrio Cholerae O139 Bengal

Isolation of Vibrio cholerae O139 from the drinking water supply during an epidemic of cholera

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