Vibrio jasicida sp. nov., a member of the Harveyi clade, isolated from marine animals (packhorse lobster, abalone and Atlantic salmon)

Yoshizawa, Susumu; Tsuruya, Yasuhiro; Fukui, Youhei; Sawabe, Tomoo; Yokota, Akira; Kogure, Kazuhiro; Higgins, Melissa; Carson, J; Thompson, Fabiano L.

doi:10.1099/ijs.0.025916-0

Cited by 40 publications

(25 citation statements)

References 27 publications

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“…Vibrionaceae are ubiquitous marine heterotrophic bacteria whose taxonomy has been extensively studied and revised, especially in the last years (29,43,44,45,46,47). In particular, the new microbiological and molecular trends have recently seen the mutual analysis of phenotypic, genotypic, and environmental variables, as these features are clearly interconnected in bacterial evolution (24,48,49).…”

Section: Discussionmentioning

confidence: 99%

Vibrio Trends in the Ecology of the Venice Lagoon

Rahman

Martino

Cardazzo

et al. 2014

Appl Environ Microbiol

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Vibrio is a very diverse genus that is responsible for different human and animal diseases. The accurate identification of Vibrio at the species level is important to assess the risks related to public health and diseases caused by aquatic organisms. The ecology of Vibrio spp., together with their genetic background, represents an important key for species discrimination and evolution. Thus, analyses of population structure and ecology association are necessary for reliable characterization of bacteria and to investigate whether bacterial species are going through adaptation processes. In this study, a population of Vibrionaceae was isolated from shellfish of the Venice lagoon and analyzed in depth to study its structure and distribution in the environment. A multilocus sequence analysis (MLSA) was developed on the basis of four housekeeping genes. Both molecular and biochemical approaches were used for species characterization, and the results were compared to assess the consistency of the two methods. In addition, strain ecology and the association between genetic information and environment were investigated through statistical models. The phylogenetic and population analyses achieved good species clustering, while biochemical identification was demonstrated to be imprecise. In addition, this study provided a fine-scale overview of the distribution of Vibrio spp. in the Venice lagoon, and the results highlighted a preferential association of the species toward specific ecological variables. These findings support the use of MLSA for taxonomic studies and demonstrate the need to consider environmental information to obtain broader and more accurate bacterial characterization. Vibrio spp. are Gram-negative halophilic bacteria belonging to the class Gammaproteobacteria. Vibrio is one of the most studied and diverse genera of microorganisms found in aquatic ecosystems and comprises the major culturable bacteria in marine and estuarine environments (1). According to the Association of Vibrio Biologists (AViB; http://www2.ioc.fiocruz.br/vibrio/AVib /species.html), there are 99 accepted or proposed Vibrio species, although the recent description of new species has led to a constantly changing taxonomy. Vibrio spp. are frequently isolated from fish, fish products, and edible shellfish, and a large number of species are pathogenic to different hosts. Some species, such as V. cholerae, V. parahaemolyticus, and V. vulnificus, cause serious food-borne gastroenteritis in humans. Other species, such as V. anguillarum and V. salmonicida, are pathogenic for fish; V. splendidus-related species are pathogenic for bivalves, and V. harveyi and V. campbellii are pathogenic for shrimps (1, 2, 3). Recently, Austin suggested a classification of zoonotic Vibrio in two classes named "higher-risk" vibrios (V. cholerae, V. parahaemolyticus, and V. vulnificus) and "lower-risk" vibrios (V. alginolyticus, V. fluvialis, V. furnissii, V. harveyi, and V. mimicus) (4). Bivalve mollusks such as clams and mussels represent products of great econ...

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Section: Discussionmentioning

confidence: 99%

Vibrio Trends in the Ecology of the Venice Lagoon

Rahman

Martino

Cardazzo

et al. 2014

Appl Environ Microbiol

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“…Balcazar et al (2012) proposed classification of the strain CECT 7692 T as the type strain of V. inhibens in 2012, but the species name was formally included in a Validation List in 2015 (Orren & Garrity, 2015). The name V. jasicida was validly described in 2012 (Yoshizawa et al, 2012) and has a priority over V. The parsimonious analysis found a single most-parsimonious tree (tree length=61 726). Numbers at the nodes are jackknife resampling values, some were omitted for clarity.…”

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confidence: 99%

“…Overall phenotypic character was found to be very similar between CECT 7692 T and two representative V. jasicida strains. Furthermore, previous studies have shown that V. inhibens CECT 7692 T has a cellular fatty acids composition very similar to that of V. jasicida (Balcazar et al, 2012;Yoshizawa et al, 2012).…”

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confidence: 99%

Genomic evidence that Vibrio inhibens is a heterotypic synonym of Vibrio jasicida

Urbanczyk

Ogura

Hayashi

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Vibrio inhibens is a marine bacterium species of the genus Vibrio (Vibrionaceae, Gammaproteobacteria). The species has been shown to be closely related to members of the genus Vibrio in the so-called Harveyi clade. The clade includes at least 11 closely related species with similar physiological and biochemical properties. Due to these similarities, species of the Harveyi clade are difficult to characterize taxonomically. Previously phenotypic and genotypic properties of the V. inhibens type strain were compared with six species of the Harveyi clade, resulting in the possibility that V. inhibens could be a synonym of a previously described species. In this study, the taxonomic status of V. inhibens was analyzed using genomic approaches. The whole-genome sequence of the type strain of V. inhibens, CECT 7692 T , was obtained and analyzed. Calculations of average nucleotide identity with the BLAST algorithm (ANIb) showed that CECT 7692 T has an ANIb of 97.5 % or higher to five strains of Vibrio. jasicida, including the type strain, but an ANIb lower than 93.5 % to other members of the Harveyi clade Vibrio. Phylogenetic analysis based on nucleotide sequences of 133 protein-coding genes showed a close evolutionary relationship of CECT 7692 T to V. jasicida. Based on these results, Vibrio inhibens is proposed to be a later heterotypic synonym of V. jasicida.Strain CECT 7692 T was isolated from feces of wild longsnouted seahorse (Hippocampus guttulatus) (Balcazar et al., 2012). Based on a phylogenetic analysis of sequences of five genes, as well as a chemophenotypic analysis the strain was taxonomically classified as representing a novel species of the genus Vibrio (family Vibrionaceae, Gammaproteobacteria), Vibrio inhibens (Balcazar et al., 2012). So far, the species type strain (CECT 7692 T =DSM 23440 T =BFLP-10 T ) is the only strain of V. inhibens described in the literature. The type strain of V. inhibens, CECT 7692 T , was obtained from the Spanish Type Culture Collection (CECT). Genomic DNA of CECT 7692 T was isolated using a DNeasy Blood and Tissue kit (Qiagen). The whole-genome sequence was generated using an Illumina MiSeq platform and a Nextera XT DNA Sample Prep kit (Illumina). Pairedend sequencing resulted in 2 367 933 reads (after quality trimming), which were assembled using Platanus assembler version 1.2.1 with the option for haploid genome (Katijani et al., 2014) into 237 scaffolds of over 300 bp in length, with approximately 132-fold coverage. The draft genome was 5 945 135 bp in length, with a DNA G+C content of 43.82 %. The CECT 7692 T genome sequence was submitted Abbreviations: MLSA, multi-locus sequence analysis; ANI, average nucleotide identity; GGDC, genome-to-genome distance comparison; ANIb, ANI calculated with the blast algorithm; ANIm, ANI calculated with the MUMmer algorithm; orthoANI, orthology ANI .V. inhibens CECT 7692 T 16S rRNA sequence obtained in this study has GenBank accession number KU360274. CECT 7692 T genome sequence has DDBJ accession numbers DF977004 to DF977445.Six supple...

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“…However, external colonization was generally lacking, suggesting that the cuticle is not a preferred microenvironment for V. owensii DY05 and that unaided invasion through the cuticle is a less likely portal of entry. Diggles et al (12) demonstrated that experimentally injured S. verreauxi phyllosomas were more susceptible to V. jasicida (formerly V. harveyi [53]) in similar immersion experiments and suggested that inadvertent injuries acquired during experimental processing could have contributed to some inconsistent mortality patterns (12).…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity and Infection Cycle of Vibrio owensii in Larviculture of the Ornate Spiny Lobster (Panulirus ornatus)

Goulden

Hall

Bourne

et al. 2012

Appl Environ Microbiol

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bThe type strain of Vibrio owensii (DY05) was isolated during an epizootic of aquaculture-reared larvae (phyllosomas) of the ornate spiny lobster (Panulirus ornatus). V. owensii DY05 was formally demonstrated to be the etiological agent of a disease causing rapid and reproducible larval mortality with pathologies similar to those seen during disease epizootics. Vectored challenge via the aquaculture live feed organism Artemia (brine shrimp) caused consistent cumulative mortality rates of 84 to 89% after 72 h, in contrast to variable mortality rates seen after immersion challenge. Histopathological examination of vector-challenged phyllosomas revealed bacterial proliferation in the midgut gland (hepatopancreas) concomitant with epithelial cell necrosis. A fluorescent-protein-labeled V. owensii DY05 transconjugant showed dispersal of single cells in the foregut and hepatopancreas 6 h postexposure, leading to colonization of the entire hepatopancreas within 18 h and eventually systemic infection. V. owensii DY05 is a marine enteropathogen highly virulent to P. ornatus phyllosoma that uses vector-mediated transmission and release from host association to a planktonic existence to perpetuate transfer. This understanding of the infection process will improve targeted biocontrol strategies and enhance the prospects of commercially viable larviculture for this valuable spiny lobster species. Major steps toward the commercialization of closed life cycle aquaculture production of the ornate spiny lobster (Panulirus ornatus) have been reported recently (36), yet nutritional deficits (41) and mortality caused by bacterial disease (5) remain major constraints to hatchery productivity. Mass mortalities of larvae (phyllosomas) are often associated with enteric vibriosis, an infection of the midgut gland (hepatopancreas) caused by Vibrio species (6, 52).Members of the genus Vibrio are natural marine inhabitants, playing important roles in nutrient cycling and forming associations with zooplankton (49). Microhabitat preferences and ecological selection may be key factors in the speciation of vibrios (20), and intensive aquaculture systems are thought to select for bacterial virulence, including traits that enhance infectivity and transmission (29,33). Accordingly, many Vibrio species are pathogenic to cultured crustacean zooplanktonic larval forms, including the three closely related species Vibrio harveyi (32, 35), V. campbellii (17, 44), and the recently described V. owensii (8).It is of paramount importance to the development of efficient disease management strategies that pathogens be identified by using experimental infection models that provide information on infection routes and infection dynamics (38). Recently, microorganisms engineered to express fluorescent proteins (FP) have significantly increased the understanding of invasive pathways and infection dynamics of pathogens, including V. anguillarum, Aeromonas hydrophila, and Edwardsiella tarda in fish models (10, 24, 30) and V. harveyi in abalone (50). Panulirus sp. p...

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Vibrio jasicida sp. nov., a member of the Harveyi clade, isolated from marine animals (packhorse lobster, abalone and Atlantic salmon)

Cited by 40 publications

References 27 publications

Vibrio Trends in the Ecology of the Venice Lagoon

Vibrio Trends in the Ecology of the Venice Lagoon

Genomic evidence that Vibrio inhibens is a heterotypic synonym of Vibrio jasicida

Pathogenicity and Infection Cycle of Vibrio owensii in Larviculture of the Ornate Spiny Lobster (Panulirus ornatus)

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