Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology

Wu, Kui; Zheng, Yangyun; Wu, Qingping; Chen, Haiying; Fu, Songzhe; Kan, Biao; Long, Yongyan; Ni, Xiansheng; Tu, Junling

doi:10.1007/s12275-019-9379-x

Cited by 11 publications

(9 citation statements)

References 29 publications

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“…We observed that the promoter activity of P epsA - lux and P mfpABC - lux were all decreased significantly in Δ robA Δ opaR and Δ robA Δ cpsQ compared to that in the WT and Δ robA strains ( Figures 6E,F ). V. parahaemolyticus can produce three types of autoinducer, including harveyi autoinducer 1, autoinducer 2, and cholerae autoinducer 1, which are synthesized by LuxM, LuxS, and CqsA, respectively ( Guo et al, 2018 ; Wu K. et al, 2019 ). To explore which autoinducer participates in the regulation of RobA to OpaR and CpsQ, we then created a series of genes deletion mutants of the autoinducer synthases.…”

Section: Resultsmentioning

confidence: 99%

Identification of LuxR Family Regulators That Integrate Into Quorum Sensing Circuit in Vibrio parahaemolyticus

Zhong

Liu

et al. 2021

Front. Microbiol.

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Vibrio parahaemolyticus is one of the most important food-borne pathogens that cause economic and public health problems worldwide. Quorum sensing (QS) is a way for the cell-cell communication between bacteria that controls a wide spectrum of processes and phenotypic behaviors. In this study, we performed a systematic research of LuxR family regulators in V. parahaemolyticus and found that they influence the bacterial growth and biofilm formation. We then established a QS reporter plasmid based on bioluminescence luxCDABE operon of Vibrio harveyi and demonstrated that several LuxR family regulators integrated into QS circuit in V. parahaemolyticus. Thereinto, a novel LuxR family regulator, named RobA, was identified as a global regulator by RNA-sequencing analyses, which affected the transcription of 515 genes in V. parahaemolyticus. Subsequent studies confirmed that RobA regulated the expression of the exopolysaccharides (EPS) synthesis cluster and thus controlled the biofilm formation. In addition, bioluminescence reporter assays showed that RobA plays a key role in the QS circuit by regulating the expression of opaR, aphA, cpsQ-mfpABC, cpsS, and scrO. We further demonstrated that the regulation of RobA to EPS and MfpABC depended on OpaR and CpsQ, which combined the QS signal with bis-(3′-5′)-cyclic dimeric GMP to construct a complex regulatory network of biofilm formation. Our data provided new insights into the bacterial QS mechanisms and biofilm formation in V. parahaemolyticus.

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Section: Resultsmentioning

confidence: 99%

Identification of LuxR Family Regulators That Integrate Into Quorum Sensing Circuit in Vibrio parahaemolyticus

Zhong

Liu

et al. 2021

Front. Microbiol.

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“…Moreover, genes encoding for cell division, lipoprotein, chemotaxis, biotin synthase, protease, hemolysin secretion, phage shock protein, iron-regulated protein, bacterioferritin and ABC transporter were down-regulated in the presence of V. alginolyticus extract. A previously study reported that motility, biofilm formation, type III and VI secretion system production (T3SS, T6SS), and capsule production of V. parahaemolyticus are directly controlled by the QS regulator OpaR (Gode-Potratz & McCarter, 2011;Kernell Burke et al, 2015;Salomon, Klimko & Orth, 2014;Wang et al, 2013;Wu et al, 2019;Zhang et al, 2012). OpaR has been known as a regulator for the expression of various virulence factors and represses cytotoxicity toward host cells (Gode-Potratz & McCarter, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…The Qrr genes are not transcribed, resulting in an expressed LuxR or OpaR regulator (Gode-Potratz & McCarter, 2011;Zhang et al, 2012). It has been reported that OpaR regulator regulates colony morphology, motility, capsule polysaccharide (CPS) production, type III and VI secretion system production (T3SS, T6SS) (Gode-Potratz & McCarter, 2011;Zhang et al, 2012;Wang et al, 2013;Salomon, Klimko & Orth, 2014;Kernell Burke et al, 2015;Wu et al, 2019). Therefore, QS is one of the alternative strategies for controlling virulence and behavior of bacteria.…”

Section: Introductionmentioning

confidence: 99%

Vibrio alginolyticus influences quorum sensing-controlled phenotypes of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus

Paopradit

Tansila

Surachat

et al. 2021

PeerJ

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Background Acute hepatopancreatic necrosis syndrome (AHPND) caused by Vibrio parahaemolyticus strain (VPAHPND) impacts the shrimp industry worldwide. With the increasing problem of antibiotic abuse, studies on quorum sensing (QS) system and anti-QS compounds bring potential breakthroughs for disease prevention and treatment. Methods In this study, the cell-free culture supernatant (CFCS) and its extract of V. alginolyticus BC25 were investigated for anti-QS activity against a reporter bacteria, Chromobacterium violaceum DMST46846. The effects of CFCS and/ or extract on motility, biofilm formation and extracellular polymeric substances (EPSs) of VPAHPND PSU5591 were evaluated. Moreover, the effects of V. alginolyticus BC25 on virulence of VPAHPND PSU5591 were investigated by shrimp challenge test. The potentially active anti-QS compounds presented in the extract and effect on gene expression of VPAHPND PSU5591 were identified. Results The CFCS of V. alginolyticus BC25 and its extract showed a significant anti-QS activity against the reporter bacteria as well as swimming and swarming motilities, biofilms, and EPSs production by VPAHPND PSU5591. Transcriptome analysis revealed that V. alginolyticus BC25 extract significantly reduced the flagella genes involved in biofilm formation and iron-controlled virulence regulatory gene of VPAHPND PSU5591. Whereas, the LuxR family transcriptional regulator gene, c-factor, a cell-cell signaling gene, and capsular polysaccharide were up-regulated. The potentially active anti-QS compounds identified in extract were Cyclo-(L-Leu-L-Pro), and Cyclo-(L-Phe-L-Pro). Furthermore, V. alginolyticus BC25 enhanced disease resistance against VPAHPND PSU5591 in tested shrimp larvae. Conclusion These findings suggest that V. alginolyticus BC25 could provide natural anti-QS and anti-biofilms compounds and has great ability to be used as biocontrol agent against VPAHPND infection in shrimp aquaculture.

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“…The quorum sensing synthase gene cqsA, which corresponds to VPA0711 in Vp strain RIMD2210633 genome, has been reported to give the signal through 3-hydroxyundecan-4 one molecule that regulates colony morphology and upregulation of another QS-associated gene opaR (Wu et al, 2019). At a low cell density of Vp in the environment and host, nitric oxide (NO) activates the master QS regulatory gene opaR.…”

Section: Role Of Biofilm and Quorum Sensing Regulators For Adaptationsmentioning

confidence: 99%

Adaptations of Vibrio parahaemolyticus to Stress During Environmental Survival, Host Colonization, and Infection

2021

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Vibrio parahaemolyticus (Vp) is an aquatic Gram-negative bacterium that may infect humans and cause gastroenteritis and wound infections. The first pandemic of Vp associated infection was caused by the serovar O3:K6 and epidemics caused by the other serovars are increasingly reported. The two major virulence factors, thermostable direct hemolysin (TDH) and/or TDH-related hemolysin (TRH), are associated with hemolysis and cytotoxicity. Vp strains lacking tdh and/or trh are avirulent and able to colonize in the human gut and cause infection using other unknown factors. This pathogen is well adapted to survive in the environment and human host using several genetic mechanisms. The presence of prophages in Vp contributes to the emergence of pathogenic strains from the marine environment. Vp has two putative type-III and type-VI secretion systems (T3SS and T6SS, respectively) located on both the chromosomes. T3SS play a crucial role during the infection process by causing cytotoxicity and enterotoxicity. T6SS contribute to adhesion, virulence associated with interbacterial competition in the gut milieu. Due to differential expression, type III secretion system 2 (encoded on chromosome-2, T3SS2) and other genes are activated and transcribed by interaction with bile salts within the host. Chromosome-1 encoded T6SS1 has been predominantly identified in clinical isolates. Acquisition of genomic islands by horizontal gene transfer provides enhanced tolerance of Vp toward several antibiotics and heavy metals. Vp consists of evolutionarily conserved targets of GTPases and kinases. Expression of these genes is responsible for the survival of Vp in the host and biochemical changes during its survival. Advanced genomic analysis has revealed that various genes are encoded in Vp pathogenicity island that control and expression of virulence in the host. In the environment, the biofilm gene expression has been positively correlated to tolerance toward aerobic, anaerobic, and micro-aerobic conditions. The genetic similarity analysis of toxin/antitoxin systems of Escherichia coli with VP genome has shown a function that could induce a viable non-culturable state by preventing cell division. A better interpretation of the Vp virulence and other mechanisms that support its environmental fitness are important for diagnosis, treatment, prevention and spread of infections. This review identifies some of the common regulatory pathways of Vp in response to different stresses that influence its survival, gut colonization and virulence.

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Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology

Cited by 11 publications

References 29 publications

Identification of LuxR Family Regulators That Integrate Into Quorum Sensing Circuit in Vibrio parahaemolyticus

Identification of LuxR Family Regulators That Integrate Into Quorum Sensing Circuit in Vibrio parahaemolyticus

Vibrio alginolyticus influences quorum sensing-controlled phenotypes of acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus

Adaptations of Vibrio parahaemolyticus to Stress During Environmental Survival, Host Colonization, and Infection

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