Vibrio paucivorans sp. nov. and Vibrio qingdaonensis sp. nov., two marine bacteria

Abstract

Two Gram-stain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacteria, designated strains DBSS07T and ZSDZ65T, were isolated from the surface sediments of the Bohai sea and Qingdao coastal seawater, respectively. Phylogenetic analysis based on 16S rRNA genes, multilocus sequence analysis (MLSA), phylogenomic analysis of single-copy gene families and whole-genome data placed DBSS07T and ZSDZ65T within the genus Vibrio . DBSS07T was most closely related to Vibrio aestivus M22T, with 97.51 % sequence similarity, whereas ZSDZ65T was most closely related to Vibrio variabilis R-40492T with 97.58 % sequence similarity. DBSS07T grew with 1–7 % (w/v) NaCl (optimum 3 %), at 16–37 °C (optimum 28 °C) and at pH 6.0–9.0 (optimum pH 7.0); whereas ZSDZ65T grew with 1–5 % (w/v) NaCl (optimum 2 %), at 16–32 °C (optimum 28 °C) and at pH 6.0–9.0 (optimum pH 8.0). Both strains shared the same major fatty acid components (more than 10 % of total fatty acids) of summed feature 3 (C16 : 1ω7c or/and C16 : 1ω6c), with different proportions. The DNA G+C contents of DBSS07T and ZSDZ65T were 44.7 and 44.3 %, respectively. On the basis of the results of polyphasic analysis, DBSS07T and ZSDZ65T are considered to represent novel species within the genus Vibrio , for which the names V. paucivorans sp. nov. (type strain, DBSS07T = KCTC 82896T= MCCC 1K06284T) and V. qingdaonensis sp. nov. (type strain, ZSDZ65T = KCTC 82893T = MCCC 1K06289T) are proposed, respectively.

Vibrio methylphosphonaticus sp. nov., a methylphosphonate-decomposing bacterium isolated from surface seawater in the Xisha Islands, PR China

“…Gas (2 ml) was extracted from the headspace using a gas-tight syringe (vici), and then measured by a gas chromatograph (Shimadzu GC-14B) equipped with a flame ionization detector [5]. The temperature range for growth was determined on MA plates by incubating at 10-45 °C (10,15,20,25,28,30,35,40,43 and 45 °C) for 5 days and at 4 °C on MA for at least 30 days. In the salinity experiment, distilled water was used to prepare synthetic marine ZoBell broth (5 g l −1 peptone, 1 g l −1 yeast extract and 0.1 g l −1 FePO 4 ).…”

“…V. aestuarianus subsp. Cardii DSM 109723 T[33]; 5, V. hippocampi BFLP-4 T[34]; 6, V. barjaei 3062 T[35]; 7, V. hangzhouensis CGMCC 1.7062 T[36]; 8, V. maritimus CAIM 1455 T[37]; 9, V. mediterranei NBRC 15635 T[38]; 10, V. thalassae MD16 T[39]; 11, V. variabilis CAIM 1454 T[37]; 12, V. qingdaonensis ZSDZ65 T[40]. All the strains are positive for oxidase and catalase, and can degrade glucose, sucrose, rhamnose and trehalose.…”

Shewanella phaeophyticola sp. nov. and Vibrio algarum sp. nov., isolated from marine brown algae

“…The genus Vibrio, with Vibrio cholerae as the type species, a member of the family Vibrionaceae, order Vibrionales, phylum Pseudomonadota, is one of the most diverse groups. As of March 2024, the genus encompasses a total of 179 species, both validly and invalidly published (https://lpsn.dsmz.de/genus/vibrio), originating from various environments, including marine and estuarine waters, as well as various organisms [10][11][12][13][14][15][16][17]. Members of the genus Vibrio are generally Gram-stain-negative, oxidase-and catalase-positive, facultative aerobic, and have straight or slightly curved cells, which may be motile or non-motile rods, with the capability to reduce nitrate to nitrite [10][11][12][13][14][15][16][17].…”