Vibrio Phage Artemius, a Novel Phage Infecting Vibrio alginolyticus

Abstract

Vibrio alginolyticus is an important pathogen of marine animals and has been the target of phage therapy applications in marine aquaculture for many years. Here, we report the isolation and partial characterization of a novel species of the Siphoviridae family, the Vibrio phage Artemius. The novel phage was species-specific and could only infect strains of V. alginolyticus. It could efficiently reduce the growth of the host bacterium at various multiplicities of infection as assessed by an in vitro lysis assay. It had a genome length of 43,349 base pairs. The complete genome has double-stranded DNA with a G + C content of 43.61%. In total, 57 ORFs were identified, of which 19 were assigned a predicted function. A genomic analysis indicated that Vibrio phage Artemius is lytic and does not harbor genes encoding toxins and antibiotic resistance determinants.

In Vitro Activity, Stability and Molecular Characterization of Eight Potent Bacteriophages Infecting Carbapenem-Resistant Klebsiella pneumoniae

“…Samples were maintained in a water bath ranging between 30–80 °C for 60 min. The method was adapted from [ 20 ]. The sensitivity of each bacteriophage to chloroform was tested using 10% chloroform concentration.…”

Comprehensive Characterization of a Novel Bacteriophage, vB_VhaS_MAG7 against a Fish Pathogenic Strain of Vibrio harveyi and Its In Vivo Efficacy in Phage Therapy Trials

“…Bacterial strains that were used in this study (Table 1) belong to the bacterial collection of Laboratory of Aquaculture Microbiology, Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Center for Marine Research (HCMR) in Heraklion, Crete. They were identified at the species level either through whole-genome sequencing (WGS) and/or through sequencing of 16s rRNA and mreB genes and PCR detection of toxR, as described in Droubogiannis et al [34]. Vibrio harveyi strain MM46 which had been isolated from diseased seabass from the same fish farm was used as a host for phage isolation.…”

“…A one-step growth assay was conducted as described in Droubogiannis et al [34]. In brief, 1 mL of a fresh culture (10 7 CFU mL −1 ) was centrifuged at 13,000 rpm for 3 min.…”

“…The Geneious assembler was used for the assembly of the paired reads using Geneious Prime software. The genome annotation pipeline, previously described by Droubogiannis et al [34], was followed. Both structural and functional annotation were conducted in the Galaxy webserver [40] environment.…”

Characterization of vB_ValM_PVA8, a broad-host-range bacteriophage infecting Vibrio alginolyticus and Vibrio parahaemolyticus

“…Most of them are phages infecting V. parahaemolyticus, V. cholerae, and V. vulnificus. Until recently, the complete genomes of several V. alginolyticus phages had been reported, including those myovirus-like phages [VP01 (Sasikala and Srinivasan, 2016), PVA1 (Cui et al, 2014), ValKK3 (Lal et al, 2016), and PVA23 (Fu et al, 2022)], those podovirus-like phages [IM271 (Li et al, 2019), VEN (Kokkari et al, 2018), ϕA318 (Lin et al, 2012), and vB_ValP_VA-RY-3 (Ren et al, 2022)], and those siphovirus-like phages [ValSw3-3 (Chen et al, 2020) and Artemius (Droubogiannis et al, 2022)]. Adding to this list, some potent V. alginolyticus phages have been documented, however, the complete genomic information of them still remain unavailable (Sasikala and Srinivasan, 2016;Le et al, 2020).…”