Vibrios dominate as culturable nitrogen-fixing bacteria of the Brazilian coral Mussismilia hispida

Chimetto, Luciane A.; Brocchi, Marcelo; Thompson, Cristiane C.; Martins, Roberta Cristina Ruedas; Ramos, Heloiza R.; Thompson, Fabiano L.

doi:10.1016/j.syapm.2008.06.001

Cited by 115 publications

(89 citation statements)

References 26 publications

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“…Recent work has shown that vibrios, including V. coralliilyticus, are ubiquitous in coral mucus (Chimetto et al, 2008;Alves et al, 2010) and often dominate the microbial community of diseased corals (Bourne and Munn, 2005;Luna et al, 2007). A similar pattern is observed at the whole reef scale as vibrios have been demonstrated to have much higher loads on unhealthy vs healthy coral reefs (Dinsdale et al, 2008).…”

Section: Introductionmentioning

confidence: 64%

Genomic and proteomic analyses of the coral pathogen Vibrio coralliilyticus reveal a diverse virulence repertoire

et al. 2011

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Vibrio coralliilyticus has been implicated as an important pathogen of coral species worldwide. In this study, the nearly complete genome of Vibrio coralliilyticus strain P1 (LMG23696) was sequenced and proteases implicated in virulence of the strain were specifically investigated. The genome sequence of P1 (5 513 256 bp in size) consisted of 5222 coding sequences and 58 RNA genes (53 tRNAs and at least 5 rRNAs). Seventeen metalloprotease and effector (vgrG, hlyA and hcp) genes were identified in the genome and expressed proteases were also detected in the secretome of P1. As the VcpA zinc-metalloprotease has been considered an important virulence factor of V. coralliilyticus, a vcpA deletion mutant was constructed to evaluate the effect of this gene in animal pathogenesis. Both wild-type and mutant (DvcpA) strains exhibited similar virulence characteristics that resulted in high mortality in Artemia and Drosophila pathogenicity bioassays and strong photosystem II inactivation of the coral dinoflagellate endosymbiont (Symbiodinium). In contrast, the DvcpA mutant demonstrated higher hemolytic activity and secreted 18 proteins not secreted by the wild type. These proteins included four types of metalloproteases, a chitinase, a hemolysin-related protein RbmC, the Hcp protein and 12 hypothetical proteins. Overall, the results of this study indicate that V. coralliilyticus strain P1 has a diverse virulence repertoire that possibly enables this bacterium to be an efficient animal pathogen.

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Section: Introductionmentioning

confidence: 64%

Genomic and proteomic analyses of the coral pathogen Vibrio coralliilyticus reveal a diverse virulence repertoire

et al. 2011

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“…Growth rates in Vibrio are among the fastest known of any bacteria, with doublings every 8-9 min recorded in some species (5). Additionally, several Vibrio species are able to fix N, a process that has a very high Fe demand (38,39). Dust additions also likely added other limiting nutrients in addition to Fe, especially phosphate, which may help to explain the continued response even at very high levels (16).…”

Section: Discussionmentioning

confidence: 99%

Saharan dust nutrients promote Vibrio bloom formation in marine surface waters

Westrich

Ebling

Landing

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Vibrio is a ubiquitous genus of marine bacteria, typically comprising a small fraction of the total microbial community in surface waters, but capable of becoming a dominant taxon in response to poorly characterized factors. Iron (Fe), often restricted by limited bioavailability and low external supply, is an essential micronutrient that can limit Vibrio growth. Vibrio species have robust metabolic capabilities and an array of Fe-acquisition mechanisms, and are able to respond rapidly to nutrient influx, yet Vibrio response to environmental pulses of Fe remains uncharacterized. Here we examined the population growth of Vibrio after natural and simulated pulses of atmospherically transported Saharan dust, an important and episodic source of Fe to tropical marine waters. As a model for opportunistic bacterial heterotrophs, we demonstrated that Vibrio proliferate in response to a broad range of dust-Fe additions at rapid timescales. Within 24 h of exposure, strains of Vibrio cholerae and Vibrio alginolyticus were able to directly use Saharan dust–Fe to support rapid growth. These findings were also confirmed with in situ field studies; arrival of Saharan dust in the Caribbean and subtropical Atlantic coincided with high levels of dissolved Fe, followed by up to a 30-fold increase of culturable Vibrio over background levels within 24 h. The relative abundance of Vibrio increased from ∼1 to ∼20% of the total microbial community. This study, to our knowledge, is the first to describe Vibrio response to Saharan dust nutrients, having implications at the intersection of marine ecology, Fe biogeochemistry, and both human and environmental health.

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“…However, in this dataset and other YBD studies (Cunning et al, 2009), Vibrionaceae were noticeably more abundant (2-10x) in healthy samples ( Supplementary Figure 1). Some Vibrios are commensal in healthy corals (Chimetto et al, 2008;Koenig et al, 2011;Krediet et al, 2013). This does not negate that it may be possible for Vibrios to become pathogenic as environmental conditions change or genomic modifications occur, such as activation, shuffling or transfer of gene cassettes (Koenig et al, 2011).…”

Section: Bacterial Community Shifts Under Different Health Statesmentioning

confidence: 99%

“…In addition, corals harbor a large diversity of bacteria, some of which appear to be host-specific (Rohwer et al, 2001;Sunagawa et al, 2010;Roder et al, 2014). Of the ones that have been studied, many are beneficial to the holobiont by promoting coral health, defense and nitrogen fixation (Lesser et al, 2004;Ritchie, 2006;Chimetto et al, 2008;Alagely et al, 2011;Lema et al, 2012). These recent findings highlight the importance of deepening our understanding of host-microbe interactions in coral reef environments (Ainsworth et al, 2009;Mouchka et al, 2010), yet high throughput genomic analyses have revealed that most of the coral-associated bacteria are unclassified .…”

Section: Introductionmentioning

confidence: 99%

Coral transcriptome and bacterial community profiles reveal distinct Yellow Band Disease states inOrbicella faveolata

et al. 2014

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Coral diseases impact reefs globally. Although we continue to describe diseases, little is known about the etiology or progression of even the most common cases. To examine a spectrum of coral health and determine factors of disease progression we examined Orbicella faveolata exhibiting signs of Yellow Band Disease (YBD), a widespread condition in the Caribbean. We used a novel combined approach to assess three members of the coral holobiont: the coral-host, associated Symbiodinium algae, and bacteria. We profiled three conditions: (1) healthy-appearing colonies (HH), (2) healthy-appearing tissue on diseased colonies (HD), and (3) diseased lesion (DD). Restriction fragment length polymorphism analysis revealed health state-specific diversity in Symbiodinium clade associations. 16S ribosomal RNA gene microarrays (PhyloChips) and O. faveolata complimentary DNA microarrays revealed the bacterial community structure and host transcriptional response, respectively. A distinct bacterial community structure marked each health state. Diseased samples were associated with two to three times more bacterial diversity. HD samples had the highest bacterial richness, which included components associated with HH and DD, as well as additional unique families. The host transcriptome under YBD revealed a reduced cellular expression of defense-and metabolism-related processes, while the neighboring HD condition exhibited an intermediate expression profile. Although HD tissue appeared visibly healthy, the microbial communities and gene expression profiles were distinct. HD should be regarded as an additional (intermediate) state of disease, which is important for understanding the progression of YBD.

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Vibrios dominate as culturable nitrogen-fixing bacteria of the Brazilian coral Mussismilia hispida

Cited by 115 publications

References 26 publications

Genomic and proteomic analyses of the coral pathogen Vibrio coralliilyticus reveal a diverse virulence repertoire

Genomic and proteomic analyses of the coral pathogen Vibrio coralliilyticus reveal a diverse virulence repertoire

Saharan dust nutrients promote Vibrio bloom formation in marine surface waters

Coral transcriptome and bacterial community profiles reveal distinct Yellow Band Disease states inOrbicella faveolata

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