Vicenin 3 ameliorates ECM degradation by regulating the MAPK pathway in SW1353 chondrocytes

Chen, Yue-Yuan; Yan, Xiaojie; Jiang, Xiaohua; Lu, Fushen; Yang, Xi; Li, Dianpeng

doi:10.3892/etm.2021.10896

Cited by 4 publications

(3 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chen et al [61] reported that vicenin-3 considerably reduced the expression levels of nitric oxide, prostaglandin E2, MMP1, MMP3, MMP13, and A disintegrinlike and metalloproteinase with thrombospondin motifs (ADAMTS). Moreover, vicenin-3 inhibited the p38 MAPK signaling pathway; the results were similar to that of SB203580 (a well-known p38 MAPK inhibitor).…”

Section: Discussionmentioning

confidence: 99%

Flavonoids as Strong Inhibitors of MAPK3: A Computational Drug Discovery Approach

Taherkhani

Khodadadi

Samie

et al. 2023

International Journal of Analytical Chemistry

View full text Add to dashboard Cite

Background. Mitogen-activated protein kinase 3 (MAPK3) mediates the onset, progression, metastasis, drug resistance, and poor prognosis in various malignancies, including glioma, liver, ovarian, thyroid, lung, breast, gastric, and oral cancers. Negative regulation of MAPK3 expression using miRNAs has led to therapeutic effects in cancer. Objectives. The present study performed molecular docking and dynamics simulation to identify potential MAPK3 inhibitors from natural flavonoids, possibly leading to drug development in cancer therapy. Methods. A computational drug discovery approach was performed using the AutoDock tool to identify potential MAPK3 inhibitors from 46 plant-based flavonoids. A cross-validation study was executed using the Schrödinger Maestro docking tool. Molecular dynamics (MD) was executed to evaluate the stability of docked poses between the top-ranked compounds and the MAPK3 catalytic domain. Interactions among the most potent MAPK3 inhibitors and residues within the receptor’s active site were studied using the BIOVIA Discovery Studio Visualizer before and after 100 ns MD simulations. Results. Kaempferol 3-rutinoside-4′-glucoside, kaempferol 3-rutinoside-7-sophoroside, rutin, and vicenin-2 exhibited a magnificent binding affinity to the receptor’s active site. In addition, the stability of the docked poses of these compounds seemed to be stable after ∼45 ns computer simulations. Conclusion. The present study suggests that kaempferol 3-rutinoside-4′-glucoside, kaempferol 3-rutinoside-7-sophoroside, rutin, and vicenin-2 could strongly bind to the MAPK3 catalytic site and could be assigned as a potent inhibitor for MAPK3. These findings may be helpful in the treatment of various cancers. However, further validation experiments are needed.

show abstract

Section: Discussionmentioning

confidence: 99%

Flavonoids as Strong Inhibitors of MAPK3: A Computational Drug Discovery Approach

Taherkhani

Khodadadi

Samie

et al. 2023

International Journal of Analytical Chemistry

View full text Add to dashboard Cite

show abstract

“…Some interesting effects have been found for these types of compounds. For example, schaftoside can inhibit the SARS-CoV-2 virus [ 23 ], vicenin-II was active against the Helicobacterium pylori infection [ 24 ] and may prevent osteoarthritis due to inhibition of extracellular matrix degradation [ 25 ], and orientin showed neuroprotective, vasodilatation, and cardioprotective activities [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Polyphenolic Composition of Carlina acaulis L. Extract and Cytotoxic Potential against Colorectal Adenocarcinoma and Cervical Cancer Cells

Sowa,

Mołdoch,

Paduch

et al. 2023

Molecules

View full text Add to dashboard Cite

Carlina acaulis is highly valued in the traditional medicine of many European countries for its diuretic, cholagogue, anthelmintic, laxative, and emetic properties. Moreover, practitioners of natural medicine indicate that it has anti-cancer potential. However, its phytochemistry is still little known. In the present study, the polyphenolic composition of the plant was investigated using ultra-high-performance liquid chromatography coupled with a high-resolution/quadrupole time-of-flight mass spectrometer (UHPLC-HR/QTOF/MS-PDA). The fractionation of the extract was carried out using liquid-liquid extraction and preparative chromatography techniques. Cytotoxicity was assessed based on neutral red and MTT assays. The obtained data showed that the species is rich in chlorogenic acids and C-glycosides of luteolin and apigenin. The total amount of chlorogenic acids was 12.6 mg/g. Among flavonoids, kaempferol dihexosidipentose and schaftoside were the most abundant, reaching approximately 3 mg/g, followed by isoorientin, vitexin-2-O-rhamnoside, and vicenin II, each with a content of approximately 2 mg/g. Furthermore, the cytotoxic potential of the plant against human colorectal adenocarcinoma (HT29) and human cervical cancer (HeLa) cells was investigated using the normal epithelial colon cell line (CCD 841CoTr) as a reference. It has been demonstrated that the ethyl acetate fraction was the most abundant in polyphenolic compounds and had the most promising anticancer activity. Further fractionation allowed for the obtaining of some subfractions that differed in phytochemical composition. The subfractions containing polyphenolic acids and flavonoids were characterized by low cytotoxicity against cancer and normal cell lines. Meanwhile, the subfraction with fatty acids was active and decreased the viability of HeLa and HT29 with minimal negative effects on CCD 841CoTr. The effect was probably linked to traumatic acid, which was present in the fraction at a concentration of 147 mg/g of dried weight. The research demonstrated the significant potential of C. acaulis as a plant with promising attributes, thus justifying further exploration of its biological activity.

show abstract

“…MMP-13 and ADAMTS-5 are important enzymes for the degradation of ECMs in OA development [19]. The MMP-13 level in AGEs-stimulated chondrocytes was markedly increased from 115.2 to 236.8 pg/mL, which was decreased to 209.5 and 158.3 pg/mL by 10 and 20 μM Cabozantinib, respectively (Figure 5A).…”

Section: Cabozantinib Repressed the Levels Of Mmp-13 And Adamts-5 In ...mentioning

confidence: 98%

Cabozantinib prevents AGEs-induced degradation of type 2 collagen and aggrecan in human chondrocytes

Dong,

Lin,

et al. 2023

Aging

View full text Add to dashboard Cite

Osteoarthritis (OA) is a joint degenerative disease commonly observed in the old population, lacks effective therapeutic methods, and markedly impacts the normal lives of patients. Degradation of extracellular matrix (ECM) is reported to participate in OA development, which is a potential target for treating OA. Cabozantinib is an inhibitor of tyrosine kinases and is recently claimed with suppressive properties against inflammation. Herein, the protective function of Cabozantinib on advanced glycation end products (AGEs)-induced damages to chondrocytes was tested. SW1353 chondrocytes were stimulated with 100 μg/ml AGEs with or without 10 and 20 μM Cabozantinib for 24 h. Signally increased reactive oxygen species (ROS) levels, declined reduced glutathione (GSH) levels, and elevated release of inflammatory cytokines were observed in AGEs-stimulated SW1353 chondrocytes, which were markedly reversed by Cabozantinib. Moreover, the notably reduced type II collagen and aggrecan levels, and increased matrix metalloproteinase-13 (MMP-13) and A Disintegrin and Metalloproteinase with Thrombospondin Motifs-5 (ADAMTS-5) levels in AGEs-stimulated SW1353 chondrocytes were largely rescued by Cabozantinib. The downregulated Sry-type high-mobility-group box 9 (SOX-9) observed in AGEs-stimulated SW1353 chondrocytes was abolished by Cabozantinib. Furthermore, the impact of Cabozantinib on type II collagen and aggrecan levels in AGEs-treated SW1353 chondrocytes was abrogated by silencing SOX-9. Collectively, Cabozantinib prevented AGEs-induced degradation of type 2 collagen and aggrecan in human chondrocytes by mediating SOX-9.

show abstract

Vicenin 3 ameliorates ECM degradation by regulating the MAPK pathway in SW1353 chondrocytes

Cited by 4 publications

References 45 publications

Flavonoids as Strong Inhibitors of MAPK3: A Computational Drug Discovery Approach

Flavonoids as Strong Inhibitors of MAPK3: A Computational Drug Discovery Approach

Polyphenolic Composition of Carlina acaulis L. Extract and Cytotoxic Potential against Colorectal Adenocarcinoma and Cervical Cancer Cells

Cabozantinib prevents AGEs-induced degradation of type 2 collagen and aggrecan in human chondrocytes

Contact Info

Product

Resources

About